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In vivo and in vitro anti-inflammatory effects of ethanol fraction from Periploca forrestii Schltr.
Chin J Integr Med. 2017 Jul; 23(7):528-534.CJ

Abstract

OBJECTIVE

To determine the anti-inflflammatory effects of an ethanol fraction of Periploca forrestii Schltr. (EFPF) and to investigate the potential mechanisms underlying in vivo and in vitro models.

METHODS

The antiinflflammatory effects of EFPF were evaluated using the xylene-induced mouse ear edema and carrageenan-induced rat paw edema models in vivo. In vitro, RAW264.7 cells were exposed to 0-800 μg/mL EFPF and the cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Then cells were treated with different concentrations of EFPF (100-400 μg/mL) and stimulated with lipopolysaccharide (LPS, 1 μg/mL) for 24 h. The supernatant was analyzed for nitric oxide (NO) using the Griess reagent, and the levels of inflflammatory mediators and cytokines were determined using enzyme-linked immunosorbent assays for prostaglandin E2 (PGE2), tumor necrosis factor α (TNF-α), interleukin (IL) 6, and IL-10. The protein expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor κB (NF-κB), and mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAPK were examined by Western blot.

RESULTS

Compared with the control group, EFPF signifificantly reduced mouse ear edema and rat paw edema rate (P<0.05 or P<0.01). Compared with the LPS group, EFPF signifificantly inhibited the LPS-stimulated production of NO, PGE2, TNF-α and IL-6 (P<0.05 or P<0.01), and increased the IL-10 production (P<0.05). EFPF also signifificantly inhibited LPS-induced protein expressions of iNOS and COX-2, suppressed the phosphorylation and degradation of inhibitor of NF-κB-α, decreased p65 level, and inhibited the phosphorylation of p38, ERK1/2 and JNK P<0.05 or P<0.01).

CONCLUSION

EFPF exerted anti-inflflammatory effect by reducing protein expressions of iNOS and COX-2 and the production of the inflflammation factors, including TNF-α, IL-6, NO and PGE2, mainly through inhibition of LPS-mediated stimulation of NF-κB and MAPK signaling pathways.

Authors+Show Affiliations

Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China.Engineering Research Center for the Development and Application of Ethnic Medicine and Chinese Medicine (Ministry of Education), Guizhou Medical University, Guiyang, 550004, China.Engineering Research Center for the Development and Application of Ethnic Medicine and Chinese Medicine (Ministry of Education), Guizhou Medical University, Guiyang, 550004, China.Engineering Research Center for the Development and Application of Ethnic Medicine and Chinese Medicine (Ministry of Education), Guizhou Medical University, Guiyang, 550004, China.Engineering Research Center for the Development and Application of Ethnic Medicine and Chinese Medicine (Ministry of Education), Guizhou Medical University, Guiyang, 550004, China.Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou, 730000, China. nijm@lzu.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28283936

Citation

Dong, Li, et al. "In Vivo and in Vitro Anti-inflammatory Effects of Ethanol Fraction From Periploca Forrestii Schltr." Chinese Journal of Integrative Medicine, vol. 23, no. 7, 2017, pp. 528-534.
Dong L, Zhang Y, Wang X, et al. In vivo and in vitro anti-inflammatory effects of ethanol fraction from Periploca forrestii Schltr. Chin J Integr Med. 2017;23(7):528-534.
Dong, L., Zhang, Y., Wang, X., Dong, Y. X., Zheng, L., Li, Y. J., & Ni, J. M. (2017). In vivo and in vitro anti-inflammatory effects of ethanol fraction from Periploca forrestii Schltr. Chinese Journal of Integrative Medicine, 23(7), 528-534. https://doi.org/10.1007/s11655-017-2803-3
Dong L, et al. In Vivo and in Vitro Anti-inflammatory Effects of Ethanol Fraction From Periploca Forrestii Schltr. Chin J Integr Med. 2017;23(7):528-534. PubMed PMID: 28283936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vivo and in vitro anti-inflammatory effects of ethanol fraction from Periploca forrestii Schltr. AU - Dong,Li, AU - Zhang,Yun, AU - Wang,Xia, AU - Dong,Yong-Xi, AU - Zheng,Lin, AU - Li,Yong-Jun, AU - Ni,Jing-Man, Y1 - 2017/03/10/ PY - 2016/10/11/received PY - 2017/3/12/pubmed PY - 2018/9/1/medline PY - 2017/3/12/entrez KW - Periploca forrestii Schltr. KW - anti-inflflammatory effect KW - mitogen-activated protein kinase KW - nuclear factor κB SP - 528 EP - 534 JF - Chinese journal of integrative medicine JO - Chin J Integr Med VL - 23 IS - 7 N2 - OBJECTIVE: To determine the anti-inflflammatory effects of an ethanol fraction of Periploca forrestii Schltr. (EFPF) and to investigate the potential mechanisms underlying in vivo and in vitro models. METHODS: The antiinflflammatory effects of EFPF were evaluated using the xylene-induced mouse ear edema and carrageenan-induced rat paw edema models in vivo. In vitro, RAW264.7 cells were exposed to 0-800 μg/mL EFPF and the cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Then cells were treated with different concentrations of EFPF (100-400 μg/mL) and stimulated with lipopolysaccharide (LPS, 1 μg/mL) for 24 h. The supernatant was analyzed for nitric oxide (NO) using the Griess reagent, and the levels of inflflammatory mediators and cytokines were determined using enzyme-linked immunosorbent assays for prostaglandin E2 (PGE2), tumor necrosis factor α (TNF-α), interleukin (IL) 6, and IL-10. The protein expressions of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), nuclear factor κB (NF-κB), and mitogen-activated protein kinases (MAPKs) including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 MAPK were examined by Western blot. RESULTS: Compared with the control group, EFPF signifificantly reduced mouse ear edema and rat paw edema rate (P<0.05 or P<0.01). Compared with the LPS group, EFPF signifificantly inhibited the LPS-stimulated production of NO, PGE2, TNF-α and IL-6 (P<0.05 or P<0.01), and increased the IL-10 production (P<0.05). EFPF also signifificantly inhibited LPS-induced protein expressions of iNOS and COX-2, suppressed the phosphorylation and degradation of inhibitor of NF-κB-α, decreased p65 level, and inhibited the phosphorylation of p38, ERK1/2 and JNK P<0.05 or P<0.01). CONCLUSION: EFPF exerted anti-inflflammatory effect by reducing protein expressions of iNOS and COX-2 and the production of the inflflammation factors, including TNF-α, IL-6, NO and PGE2, mainly through inhibition of LPS-mediated stimulation of NF-κB and MAPK signaling pathways. SN - 1672-0415 UR - https://www.unboundmedicine.com/medline/citation/28283936/In_vivo_and_in_vitro_anti_inflammatory_effects_of_ethanol_fraction_from_Periploca_forrestii_Schltr_ L2 - https://dx.doi.org/10.1007/s11655-017-2803-3 DB - PRIME DP - Unbound Medicine ER -