Tags

Type your tag names separated by a space and hit enter

Quantitative proteomic analysis of extracellular matrix extracted from mono- and dual-species biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis.
Anaerobe. 2017 Apr; 44:133-142.A

Abstract

The Gram-negative bacteria Fusobacterium nucleatum and Porphyromonas gingivalis are members of a complex dental biofilm associated with periodontal disease. In this study, we cultured F. nucleatum and P. gingivalis as mono- and dual-species biofilms, and analyzed the protein composition of the biofilms extracellular polymeric matrix (EPM) by high-resolution liquid chromatography-tandem mass spectrometry. Label-free quantitative proteomic analysis was used for identification of proteins and sequence-based functional characterization for their classification and prediction of possible roles in EPM. We identified 542, 93 and 280 proteins in the matrix of F. nucleatum, P. gingivalis, and the dual-species biofilm, respectively. Nearly 70% of all EPM proteins in the dual-species biofilm originated from F. nucleatum, and a majority of these were cytoplasmic proteins, suggesting an enhanced lysis of F. nucleatum cells. The proteomic analysis also indicated an interaction between the two species: 22 F. nucleatum proteins showed differential levels between the mono and dual-species EPMs, and 11 proteins (8 and 3 from F. nucleatum and P. gingivalis, respectively) were exclusively detected in the dual-species EPM. Oxidoreductases and chaperones were among the most abundant proteins identified in all three EPMs. The biofilm matrices in addition contained several known and hypothetical virulence proteins, which can mediate adhesion to the host cells and disintegration of the periodontal tissues. This study demonstrated that the biofilm matrix of two important periodontal pathogens consists of a multitude of proteins whose amounts and functionalities vary largely. Relatively high levels of several of the detected proteins might facilitate their potential use as targets for the inhibition of biofilm development.

Authors+Show Affiliations

The Gade Research Group for Infection and Immunity, Department of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway. Electronic address: Marwan.Mohammed@uib.no.The Gade Research Group for Infection and Immunity, Department of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway. Electronic address: Veronika.Kucharova@uib.no.The Gade Research Group for Infection and Immunity, Department of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway. Electronic address: Audun.Nerland@uib.no.The Gade Research Group for Infection and Immunity, Department of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway. Electronic address: Harald.Wiker@uib.no.The Gade Research Group for Infection and Immunity, Department of Clinical Science, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway. Electronic address: Vidar.Bakken@uib.no.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28285095

Citation

Mohammed, Marwan Mansoor Ali, et al. "Quantitative Proteomic Analysis of Extracellular Matrix Extracted From Mono- and Dual-species Biofilms of Fusobacterium Nucleatum and Porphyromonas Gingivalis." Anaerobe, vol. 44, 2017, pp. 133-142.
Mohammed MMA, Pettersen VK, Nerland AH, et al. Quantitative proteomic analysis of extracellular matrix extracted from mono- and dual-species biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis. Anaerobe. 2017;44:133-142.
Mohammed, M. M. A., Pettersen, V. K., Nerland, A. H., Wiker, H. G., & Bakken, V. (2017). Quantitative proteomic analysis of extracellular matrix extracted from mono- and dual-species biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis. Anaerobe, 44, 133-142. https://doi.org/10.1016/j.anaerobe.2017.03.002
Mohammed MMA, et al. Quantitative Proteomic Analysis of Extracellular Matrix Extracted From Mono- and Dual-species Biofilms of Fusobacterium Nucleatum and Porphyromonas Gingivalis. Anaerobe. 2017;44:133-142. PubMed PMID: 28285095.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative proteomic analysis of extracellular matrix extracted from mono- and dual-species biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis. AU - Mohammed,Marwan Mansoor Ali, AU - Pettersen,Veronika Kuchařová, AU - Nerland,Audun H, AU - Wiker,Harald G, AU - Bakken,Vidar, Y1 - 2017/03/09/ PY - 2016/11/09/received PY - 2017/02/02/revised PY - 2017/03/03/accepted PY - 2017/3/13/pubmed PY - 2017/4/25/medline PY - 2017/3/13/entrez KW - Bacterial biofilms KW - Extracellular polymeric matrix KW - Fusobacterium nucleatum KW - Label-free quantitative proteomics KW - Porphyromonas gingivalis KW - Tandem mass spectrometry SP - 133 EP - 142 JF - Anaerobe JO - Anaerobe VL - 44 N2 - The Gram-negative bacteria Fusobacterium nucleatum and Porphyromonas gingivalis are members of a complex dental biofilm associated with periodontal disease. In this study, we cultured F. nucleatum and P. gingivalis as mono- and dual-species biofilms, and analyzed the protein composition of the biofilms extracellular polymeric matrix (EPM) by high-resolution liquid chromatography-tandem mass spectrometry. Label-free quantitative proteomic analysis was used for identification of proteins and sequence-based functional characterization for their classification and prediction of possible roles in EPM. We identified 542, 93 and 280 proteins in the matrix of F. nucleatum, P. gingivalis, and the dual-species biofilm, respectively. Nearly 70% of all EPM proteins in the dual-species biofilm originated from F. nucleatum, and a majority of these were cytoplasmic proteins, suggesting an enhanced lysis of F. nucleatum cells. The proteomic analysis also indicated an interaction between the two species: 22 F. nucleatum proteins showed differential levels between the mono and dual-species EPMs, and 11 proteins (8 and 3 from F. nucleatum and P. gingivalis, respectively) were exclusively detected in the dual-species EPM. Oxidoreductases and chaperones were among the most abundant proteins identified in all three EPMs. The biofilm matrices in addition contained several known and hypothetical virulence proteins, which can mediate adhesion to the host cells and disintegration of the periodontal tissues. This study demonstrated that the biofilm matrix of two important periodontal pathogens consists of a multitude of proteins whose amounts and functionalities vary largely. Relatively high levels of several of the detected proteins might facilitate their potential use as targets for the inhibition of biofilm development. SN - 1095-8274 UR - https://www.unboundmedicine.com/medline/citation/28285095/Quantitative_proteomic_analysis_of_extracellular_matrix_extracted_from_mono__and_dual_species_biofilms_of_Fusobacterium_nucleatum_and_Porphyromonas_gingivalis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1075-9964(17)30045-8 DB - PRIME DP - Unbound Medicine ER -