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Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology.
Parasit Vectors. 2017 Mar 13; 10(1):113.PV

Abstract

BACKGROUND

Infection and clinical disease associated with Leishmania major and Leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. This study describes dogs infected with these Leishmania spp. prevalent in the Middle East and North Africa, and compares the serological response of dogs infected with Leishmania infantum, L. major or L. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (ELISA) of each species and to rK39 dipstick.

RESULTS

Leishmania major infection in a 5-month-old male dog was associated with alopecic and ulcerative periocular and limb skin lesions which responded to allopurinol treatment. Infection was detected by skin and blood polymerase chain reaction (PCR) and confirmed by DNA sequencing but the dog was seronegative. Leishmania tropica infection was detected in a 3-month-old female dog co-infected with Babesia vogeli and Anaplasma platys and with no skin lesions. PCR and DNA sequencing of the blood and parasite culture were positive for L. tropica. Sera from 11 dogs infected with L. infantum, L. major or L. tropica were reactive with all three Leishmania spp. antigens except for sera from a dog with L. major infection. No significant differences were found between reactivity of dog sera to the antigen of the infecting species, or to the other Leishmania spp. antigens. Sera from dogs infected with L. infantum and L. tropica were positive with the rK39 antigen kit, while dogs with L. major infection were seronegative.

CONCLUSIONS

Skin lesions in L. major infected dogs from this study and previous reports (n = 2) were ulcerative and located on the muzzle, feet and foot pads and not associated with generalized lymphadenomegaly and splenomegaly. In previous L. tropica infections, skin lesions were proliferative mucocutaneous in young dogs (n = 2), or associated with widespread dermatitis, lymphadenomegaly and splenomegaly in older dogs with similarity to L. infantum infection (n = 2). This study suggests that ELISA serology with whole promastigote antigen is not distinctive between L. infantum, L. major and L. tropica canine infections and that some L. major infections are not seropositive. PCR with DNA sequencing should be used to discriminate between canine infections with these three species.

Authors+Show Affiliations

School of Veterinary Medicine, Hebrew University, P.O. Box 12, Rehovot, 76100, Israel. gad.baneth@mail.huji.ac.il.School of Veterinary Medicine, Hebrew University, P.O. Box 12, Rehovot, 76100, Israel.School of Veterinary Medicine, Hebrew University, P.O. Box 12, Rehovot, 76100, Israel.School of Veterinary Medicine, Hebrew University, P.O. Box 12, Rehovot, 76100, Israel.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

28285601

Citation

Baneth, Gad, et al. "Canine Leishmaniosis Caused By Leishmania Major and Leishmania Tropica: Comparative Findings and Serology." Parasites & Vectors, vol. 10, no. 1, 2017, p. 113.
Baneth G, Yasur-Landau D, Gilad M, et al. Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology. Parasit Vectors. 2017;10(1):113.
Baneth, G., Yasur-Landau, D., Gilad, M., & Nachum-Biala, Y. (2017). Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology. Parasites & Vectors, 10(1), 113. https://doi.org/10.1186/s13071-017-2050-7
Baneth G, et al. Canine Leishmaniosis Caused By Leishmania Major and Leishmania Tropica: Comparative Findings and Serology. Parasit Vectors. 2017 Mar 13;10(1):113. PubMed PMID: 28285601.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Canine leishmaniosis caused by Leishmania major and Leishmania tropica: comparative findings and serology. AU - Baneth,Gad, AU - Yasur-Landau,Daniel, AU - Gilad,Matan, AU - Nachum-Biala,Yaarit, Y1 - 2017/03/13/ PY - 2017/02/13/received PY - 2017/02/20/accepted PY - 2017/3/14/entrez PY - 2017/3/14/pubmed PY - 2017/9/16/medline KW - Canine leishmaniosis KW - Co-infections KW - Cutaneous leishmaniosis KW - Israel KW - Leishmania major KW - Leishmania tropica SP - 113 EP - 113 JF - Parasites & vectors JO - Parasit Vectors VL - 10 IS - 1 N2 - BACKGROUND: Infection and clinical disease associated with Leishmania major and Leishmania tropica, two common agents of human cutaneous leishmaniosis, have rarely been reported in dogs. This study describes dogs infected with these Leishmania spp. prevalent in the Middle East and North Africa, and compares the serological response of dogs infected with Leishmania infantum, L. major or L. tropica to whole promastigote antigen enzyme-linked immunosorbent assay (ELISA) of each species and to rK39 dipstick. RESULTS: Leishmania major infection in a 5-month-old male dog was associated with alopecic and ulcerative periocular and limb skin lesions which responded to allopurinol treatment. Infection was detected by skin and blood polymerase chain reaction (PCR) and confirmed by DNA sequencing but the dog was seronegative. Leishmania tropica infection was detected in a 3-month-old female dog co-infected with Babesia vogeli and Anaplasma platys and with no skin lesions. PCR and DNA sequencing of the blood and parasite culture were positive for L. tropica. Sera from 11 dogs infected with L. infantum, L. major or L. tropica were reactive with all three Leishmania spp. antigens except for sera from a dog with L. major infection. No significant differences were found between reactivity of dog sera to the antigen of the infecting species, or to the other Leishmania spp. antigens. Sera from dogs infected with L. infantum and L. tropica were positive with the rK39 antigen kit, while dogs with L. major infection were seronegative. CONCLUSIONS: Skin lesions in L. major infected dogs from this study and previous reports (n = 2) were ulcerative and located on the muzzle, feet and foot pads and not associated with generalized lymphadenomegaly and splenomegaly. In previous L. tropica infections, skin lesions were proliferative mucocutaneous in young dogs (n = 2), or associated with widespread dermatitis, lymphadenomegaly and splenomegaly in older dogs with similarity to L. infantum infection (n = 2). This study suggests that ELISA serology with whole promastigote antigen is not distinctive between L. infantum, L. major and L. tropica canine infections and that some L. major infections are not seropositive. PCR with DNA sequencing should be used to discriminate between canine infections with these three species. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/28285601/Canine_leishmaniosis_caused_by_Leishmania_major_and_Leishmania_tropica:_comparative_findings_and_serology_ DB - PRIME DP - Unbound Medicine ER -