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Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa.
J Reprod Fertil 1987; 81(2):459-69JR

Abstract

Addition of the divalent cation ionophore, A23187, to washed populations of human spermatozoa resulted in a sudden burst of production of reactive oxygen species which peaked within 3-5 min. This activity was dependent upon the presence of calcium in the external medium and was unaffected by the mitochondrial inhibitors, oligomycin, antimycin and rotenone. Studies with scavengers of reactive oxygen species revealed that, while reagents directed against singlet oxygen and the hydroxyl radical were without effect, cytochrome C reduced the response to A23187 by about 50%, suggesting that the superoxide anion radical is a major product of the activated human spermatozoon. The clinical implications of these studies stem from the considerable variation observed between individuals in the levels of reactive oxygen species produced by the spermatozoa. This variability was shown to be inversely related to the ability of the spermatozoa to exhibit sperm-oocyte fusion on exposure to A23187; defective samples exhibited a basal level of reactive oxygen species production which was 40 times that observed with normal functional cells.

Authors+Show Affiliations

MRC Reproductive Biology Unit, Edinburgh, U.K.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2828610

Citation

Aitken, R J., and J S. Clarkson. "Cellular Basis of Defective Sperm Function and Its Association With the Genesis of Reactive Oxygen Species By Human Spermatozoa." Journal of Reproduction and Fertility, vol. 81, no. 2, 1987, pp. 459-69.
Aitken RJ, Clarkson JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil. 1987;81(2):459-69.
Aitken, R. J., & Clarkson, J. S. (1987). Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. Journal of Reproduction and Fertility, 81(2), pp. 459-69.
Aitken RJ, Clarkson JS. Cellular Basis of Defective Sperm Function and Its Association With the Genesis of Reactive Oxygen Species By Human Spermatozoa. J Reprod Fertil. 1987;81(2):459-69. PubMed PMID: 2828610.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. AU - Aitken,R J, AU - Clarkson,J S, PY - 1987/11/1/pubmed PY - 1987/11/1/medline PY - 1987/11/1/entrez SP - 459 EP - 69 JF - Journal of reproduction and fertility JO - J. Reprod. Fertil. VL - 81 IS - 2 N2 - Addition of the divalent cation ionophore, A23187, to washed populations of human spermatozoa resulted in a sudden burst of production of reactive oxygen species which peaked within 3-5 min. This activity was dependent upon the presence of calcium in the external medium and was unaffected by the mitochondrial inhibitors, oligomycin, antimycin and rotenone. Studies with scavengers of reactive oxygen species revealed that, while reagents directed against singlet oxygen and the hydroxyl radical were without effect, cytochrome C reduced the response to A23187 by about 50%, suggesting that the superoxide anion radical is a major product of the activated human spermatozoon. The clinical implications of these studies stem from the considerable variation observed between individuals in the levels of reactive oxygen species produced by the spermatozoa. This variability was shown to be inversely related to the ability of the spermatozoa to exhibit sperm-oocyte fusion on exposure to A23187; defective samples exhibited a basal level of reactive oxygen species production which was 40 times that observed with normal functional cells. SN - 0022-4251 UR - https://www.unboundmedicine.com/medline/citation/2828610/Cellular_basis_of_defective_sperm_function_and_its_association_with_the_genesis_of_reactive_oxygen_species_by_human_spermatozoa_ L2 - https://www.lens.org/lens/search?q=citation_id:2828610 DB - PRIME DP - Unbound Medicine ER -