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First Report of the East-Central South African Genotype of Chikungunya Virus in Rio de Janeiro, Brazil.

Abstract

BACKGROUND

Chikungunya virus (CHIKV) is an arbovirus that causes an acute febrile syndrome with a severe and debilitating arthralgia. In Brazil, the Asian and East-Central South African (ECSA) genotypes are circulating in the north and northeast of the country, respectively. In 2015, the first autochthonous cases in Rio de Janeiro, Brazil were reported but until now the circulating strains have not been characterized. Therefore, we aimed here to perform the molecular characterization and phylogenetic analysis of CHIKV strains circulating in the 2016 outbreak occurred in the municipality of Rio de Janeiro.

METHODS

The cases analyzed in this study were collected at a private Hospital, from April 2016 to May 2016, during the chikungunya outbreak in Rio de Janeiro, Brazil. All cases were submitted to the Real Time RT-PCR for CHIKV genome detection and to anti-CHIKV IgM ELISA. Chikungunya infection was laboratorially confirmed by at least one diagnostic method and, randomly selected positive cases (n=10), were partially sequenced (CHIKV E1 gene) and analyzed.

RESULTS

The results showed that all the samples grouped in ECSA genotype branch and the molecular characterization of the fragment did not reveal the A226V mutation in the Rio de Janeiro strains analyzed, but a K211T amino acid substitution was observed for the first time in all samples and a V156A substitution in two of ten samples.

CONCLUSIONS

Phylogenetic analysis and molecular characterization reveals the circulation of the ECSA genotype of CHIKV in the city of Rio de Janeiro, Brazil and two amino acids substitutions (K211T and V156A) exclusive to the CHIKV strains obtained during the 2016 epidemic, were reported.

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  • Authors+Show Affiliations

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Rio-Laranjeiras Hospital, Gaffrée Guinle University Hospital (Federal University of the State of Rio de Janeiro), Rio de Janeiro, Brazil Pedro Ernesto University Hospital (University of the State of Rio de Janeiro), Rio de Janeiro, Brazil.

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    Rio-Laranjeiras Hospital, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil; UFRJ- Federal University of Rio de Janeiro, Laboratory of Genetics, IPPMG - Martagão Gesteira Child Care and Pediatrics Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil. Mosquitoes Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Flavivirus Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

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    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

    Viral Immunology Laboratory, Oswaldo Cruz Institute, Rio de Janeiro, Brazil.

    Source

    PLoS currents 9: 2017 Feb 14 pg

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    28286701