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Flavan-3-ols and proanthocyanidins from Limonium brasiliense inhibit the adhesion of Porphyromonas gingivalis to epithelial host cells by interaction with gingipains.
Fitoterapia. 2017 Apr; 118:87-93.F

Abstract

Porphyromonas gingivalis is a pathogen strongly involved in chronic and aggressive forms of periodontitis. Natural products, mainly polyphenols, have been described for advanced treatment of periodontitis by inhibition of the bacterial adhesion of P. gingivalis to the epithelial host cells. An acetone:water extract (LBE) from the rhizomes of Limonium brasiliense (Boiss.) Kuntze was tested under in vitro conditions for potential antiadhesive effects against P. gingivalis to human KB cells and for inhibition of the proteolytic activity of gingipains, the main virulence factor of P. gingivalis. LBE≤100μg/mL had no cytotoxicity against the bacteria and did not influence the cell physiology of human epithelial KB cells. At 100μg/mL LBE reduced the adhesion of P. gingivalis to KB cells significantly by about 80%. LBE at 20μg/mL reduced the proteolytic activity of the arginin-specific Rgp gingipain by about 75%. Chemical profiling of LBE indicated the presence of gallic acid, epigallocatechin-3-O-gallate and samarangenins A and B as lead compounds. UHPLC by using MS and UV detection displays a suitable method for quality control of the extract for identification and quantification of the lead compounds.

Authors+Show Affiliations

State University of Maringá, Postgraduate Program in Biological Sciences, Laboratory of Technological Innovation in the Development of Drugs and Cosmetics, BR-87020-900, Maringá, Brazil.University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstraβe 48, D-48149 Münster, Germany. Electronic address: ahensel@uni-muenster.de.State University of Maringá, Postgraduate Program in Pharmaceutical Sciences, Pharmaceutical Biology Laboratory, Palafito, BR-87020-900, Maringá, Brazil.State University of Maringá, Postgraduate Program in Pharmaceutical Sciences, Pharmaceutical Biology Laboratory, Palafito, BR-87020-900, Maringá, Brazil.State University of Maringá, Postgraduate Program in Pharmaceutical Sciences, Laboratory of Technological Innovation in the Development of Drugs and Cosmetics, BR-87020-900, Maringá, Brazil.University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstraβe 48, D-48149 Münster, Germany.University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstraβe 48, D-48149 Münster, Germany.State University of Maringá, Postgraduate Program in Biological Sciences, Laboratory of Technological Innovation in the Development of Drugs and Cosmetics, BR-87020-900, Maringá, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28288871

Citation

de Oliveira Caleare, Angelo, et al. "Flavan-3-ols and Proanthocyanidins From Limonium Brasiliense Inhibit the Adhesion of Porphyromonas Gingivalis to Epithelial Host Cells By Interaction With Gingipains." Fitoterapia, vol. 118, 2017, pp. 87-93.
de Oliveira Caleare A, Hensel A, Mello JC, et al. Flavan-3-ols and proanthocyanidins from Limonium brasiliense inhibit the adhesion of Porphyromonas gingivalis to epithelial host cells by interaction with gingipains. Fitoterapia. 2017;118:87-93.
de Oliveira Caleare, A., Hensel, A., Mello, J. C., Pinha, A. B., Panizzon, G. P., Lechtenberg, M., Petereit, F., & Nakamura, C. V. (2017). Flavan-3-ols and proanthocyanidins from Limonium brasiliense inhibit the adhesion of Porphyromonas gingivalis to epithelial host cells by interaction with gingipains. Fitoterapia, 118, 87-93. https://doi.org/10.1016/j.fitote.2017.03.002
de Oliveira Caleare A, et al. Flavan-3-ols and Proanthocyanidins From Limonium Brasiliense Inhibit the Adhesion of Porphyromonas Gingivalis to Epithelial Host Cells By Interaction With Gingipains. Fitoterapia. 2017;118:87-93. PubMed PMID: 28288871.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flavan-3-ols and proanthocyanidins from Limonium brasiliense inhibit the adhesion of Porphyromonas gingivalis to epithelial host cells by interaction with gingipains. AU - de Oliveira Caleare,Angelo, AU - Hensel,Andreas, AU - Mello,João Carlos Palazzo, AU - Pinha,Andressa Blainski, AU - Panizzon,Gean Pier, AU - Lechtenberg,Matthias, AU - Petereit,Frank, AU - Nakamura,Celso Vataru, Y1 - 2017/03/11/ PY - 2017/01/17/received PY - 2017/02/17/revised PY - 2017/03/10/accepted PY - 2017/3/16/pubmed PY - 2017/4/26/medline PY - 2017/3/15/entrez KW - Adhesion KW - Cysteine proteases KW - Gingipains KW - Limonium brasiliense KW - Periodontitis KW - Porphyromonas gingivalis SP - 87 EP - 93 JF - Fitoterapia JO - Fitoterapia VL - 118 N2 - Porphyromonas gingivalis is a pathogen strongly involved in chronic and aggressive forms of periodontitis. Natural products, mainly polyphenols, have been described for advanced treatment of periodontitis by inhibition of the bacterial adhesion of P. gingivalis to the epithelial host cells. An acetone:water extract (LBE) from the rhizomes of Limonium brasiliense (Boiss.) Kuntze was tested under in vitro conditions for potential antiadhesive effects against P. gingivalis to human KB cells and for inhibition of the proteolytic activity of gingipains, the main virulence factor of P. gingivalis. LBE≤100μg/mL had no cytotoxicity against the bacteria and did not influence the cell physiology of human epithelial KB cells. At 100μg/mL LBE reduced the adhesion of P. gingivalis to KB cells significantly by about 80%. LBE at 20μg/mL reduced the proteolytic activity of the arginin-specific Rgp gingipain by about 75%. Chemical profiling of LBE indicated the presence of gallic acid, epigallocatechin-3-O-gallate and samarangenins A and B as lead compounds. UHPLC by using MS and UV detection displays a suitable method for quality control of the extract for identification and quantification of the lead compounds. SN - 1873-6971 UR - https://www.unboundmedicine.com/medline/citation/28288871/Flavan_3_ols_and_proanthocyanidins_from_Limonium_brasiliense_inhibit_the_adhesion_of_Porphyromonas_gingivalis_to_epithelial_host_cells_by_interaction_with_gingipains_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0367-326X(17)30111-9 DB - PRIME DP - Unbound Medicine ER -