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[Mobilization of phase I plasmid in Shigella sonnei and stability of its inheritance in rough strains of Shigella and Escherichia].

Abstract

The plasmid pSS120, determining the synthesis of species specific I phase antigen of Shigella sonnei is mobilized for genetic transfer into E. coli K12 recipient cells with the frequency 12-41%. The frequency depends on the type of mobilized plasmid and recipient strain. The I phase antigen is normally expressed in II phase recipient cells and in E. coli cells. During mobilization pSS120 forms cointegrates representing a recombinant of mobilizing and mobilized plasmids DNA. The study of pSS120 inheritance stability has shown the plasmid to be unstable during culturing of bacteria and to be partially lost from the parent Shigella sonnei strains as well as from the "hybrid" transconjugants obtained. The 60 Md plasmid present in the donor strains of Shigella sonnei is prone to structural fragmentation particularly expressed in Shigella sonnei/E. coli hybrids.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

2828937

Citation

Romanova, Iu M., et al. "[Mobilization of Phase I Plasmid in Shigella Sonnei and Stability of Its Inheritance in Rough Strains of Shigella and Escherichia]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1987, pp. 19-24.
Romanova IuM, Nastichkin IA, Martsinkovskaia AI, et al. [Mobilization of phase I plasmid in Shigella sonnei and stability of its inheritance in rough strains of Shigella and Escherichia]. Mol Gen Mikrobiol Virusol. 1987.
Romanova, I. u. M., Nastichkin, I. A., Martsinkovskaia, A. I., Rudnev, I. A., & Petrovskaia, V. G. (1987). [Mobilization of phase I plasmid in Shigella sonnei and stability of its inheritance in rough strains of Shigella and Escherichia]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (9), 19-24.
Romanova IuM, et al. [Mobilization of Phase I Plasmid in Shigella Sonnei and Stability of Its Inheritance in Rough Strains of Shigella and Escherichia]. Mol Gen Mikrobiol Virusol. 1987;(9)19-24. PubMed PMID: 2828937.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Mobilization of phase I plasmid in Shigella sonnei and stability of its inheritance in rough strains of Shigella and Escherichia]. AU - Romanova,Iu M, AU - Nastichkin,I A, AU - Martsinkovskaia,A I, AU - Rudnev,I A, AU - Petrovskaia,V G, PY - 1987/9/1/pubmed PY - 1987/9/1/medline PY - 1987/9/1/entrez SP - 19 EP - 24 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol. Gen. Mikrobiol. Virusol. IS - 9 N2 - The plasmid pSS120, determining the synthesis of species specific I phase antigen of Shigella sonnei is mobilized for genetic transfer into E. coli K12 recipient cells with the frequency 12-41%. The frequency depends on the type of mobilized plasmid and recipient strain. The I phase antigen is normally expressed in II phase recipient cells and in E. coli cells. During mobilization pSS120 forms cointegrates representing a recombinant of mobilizing and mobilized plasmids DNA. The study of pSS120 inheritance stability has shown the plasmid to be unstable during culturing of bacteria and to be partially lost from the parent Shigella sonnei strains as well as from the "hybrid" transconjugants obtained. The 60 Md plasmid present in the donor strains of Shigella sonnei is prone to structural fragmentation particularly expressed in Shigella sonnei/E. coli hybrids. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/2828937/[Mobilization_of_phase_I_plasmid_in_Shigella_sonnei_and_stability_of_its_inheritance_in_rough_strains_of_Shigella_and_Escherichia]_ DB - PRIME DP - Unbound Medicine ER -