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Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.).
Theor Appl Genet. 2017 May; 130(5):891-902.TA

Abstract

KEY MESSAGE

Recombination at the Glu-3 loci was identified, and strong genetic linkage was observed only between the amplicons representing i-type and s-type genes located, respectively, at the Glu-A3 and Glu-B3 loci. The low-molecular weight glutenin subunits (LMW-GSs) are one of the major components of wheat seed storage proteins and play a critical role in the determination of wheat end-use quality. The genes encoding this class of proteins are located at the orthologous Glu-3 loci (Glu-A3, Glu-B3, and Glu-D3). Due to the complexity of these chromosomal regions and the high sequence similarity between different LMW-GS genes, their organization and recombination characteristics are still incompletely understood. This study examined intralocus recombination at the Glu-3 loci in two recombinant inbred line (RIL) and one doubled haploid (DH) population, all segregating for the Glu-A3, Glu-B3, and Glu-D3 loci. The analysis was conducted using a gene marker system that consists of the amplification of the complete set of the LMW-GS genes and their visualization by capillary electrophoresis. Recombinant marker haplotypes were detected in all three populations with different recombination rates depending on the locus and the population. No recombination was observed between the amplicons representing i-type and s-type LMW-GS genes located, respectively, at the Glu-A3 and Glu-B3 loci, indicating tight linkage between these genes. Results of this study contribute to better understanding the genetic linkage and recombination between different LMW-GS genes, the structure of the Glu-3 loci, and the development of more specific molecular markers that better represent the genetic diversity of these loci. In this way, a more precise analysis of the contribution of various LMW-GSs to end-use quality of wheat may be achieved.

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Authors+Show Affiliations

Ibba MI
Department of Crop and Soil Sciences, Washington State University, Pullman, WA, USA.
Kiszonas AM
USDA-ARS Western Wheat Quality Laboratory, Pullman, WA, USA.
Morris CF
USDA-ARS Western Wheat Quality Laboratory, Pullman, WA, USA. craig.morris@ars.usda.gov.

MeSH

Chromosome MappingGenetic LinkageGenetic LociGlutensHaplotypesRecombination, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28289804

Citation

Ibba, Maria I., et al. "Evidence of Intralocus Recombination at the Glu-3 Loci in Bread Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 5, 2017, pp. 891-902.
Ibba MI, Kiszonas AM, Morris CF. Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.). Theor Appl Genet. 2017;130(5):891-902.
Ibba, M. I., Kiszonas, A. M., & Morris, C. F. (2017). Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(5), 891-902. https://doi.org/10.1007/s00122-017-2858-8
Ibba MI, Kiszonas AM, Morris CF. Evidence of Intralocus Recombination at the Glu-3 Loci in Bread Wheat (Triticum Aestivum L.). Theor Appl Genet. 2017;130(5):891-902. PubMed PMID: 28289804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.). AU - Ibba,Maria I, AU - Kiszonas,Alecia M, AU - Morris,Craig F, Y1 - 2017/03/13/ PY - 2016/10/03/received PY - 2017/01/17/accepted PY - 2017/3/16/pubmed PY - 2017/5/26/medline PY - 2017/3/15/entrez SP - 891 EP - 902 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 5 N2 - KEY MESSAGE: Recombination at the Glu-3 loci was identified, and strong genetic linkage was observed only between the amplicons representing i-type and s-type genes located, respectively, at the Glu-A3 and Glu-B3 loci. The low-molecular weight glutenin subunits (LMW-GSs) are one of the major components of wheat seed storage proteins and play a critical role in the determination of wheat end-use quality. The genes encoding this class of proteins are located at the orthologous Glu-3 loci (Glu-A3, Glu-B3, and Glu-D3). Due to the complexity of these chromosomal regions and the high sequence similarity between different LMW-GS genes, their organization and recombination characteristics are still incompletely understood. This study examined intralocus recombination at the Glu-3 loci in two recombinant inbred line (RIL) and one doubled haploid (DH) population, all segregating for the Glu-A3, Glu-B3, and Glu-D3 loci. The analysis was conducted using a gene marker system that consists of the amplification of the complete set of the LMW-GS genes and their visualization by capillary electrophoresis. Recombinant marker haplotypes were detected in all three populations with different recombination rates depending on the locus and the population. No recombination was observed between the amplicons representing i-type and s-type LMW-GS genes located, respectively, at the Glu-A3 and Glu-B3 loci, indicating tight linkage between these genes. Results of this study contribute to better understanding the genetic linkage and recombination between different LMW-GS genes, the structure of the Glu-3 loci, and the development of more specific molecular markers that better represent the genetic diversity of these loci. In this way, a more precise analysis of the contribution of various LMW-GSs to end-use quality of wheat may be achieved. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/28289804/Evidence_of_intralocus_recombination_at_the_Glu_3_loci_in_bread_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-017-2858-8 DB - PRIME DP - Unbound Medicine ER -