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A low copy number cosmid.
Plasmid 1987; 18(2):170-2P

Abstract

A low copy number cosmid was constructed by subcloning the pair of cos sites and the kanamycin resistance gene of pcos2EMBL into pGB2. The resulting cosmid, pPR691, has the pSC101 replicon and specifies resistance to kanamycin, spectinomycin, and streptomycin. pPR691 also carries restriction sites suitable for cloning partial Sau3A digests using the strategy of Bates and Swift (P. F. Bates and R. A. Swift, 1983, Gene 26, 137-146). A library of Salmonella typhimurium chromosomal DNA was made using this cosmid and the rfb gene cluster (map position 42) was isolated from this library.

Authors+Show Affiliations

Department of Microbiology, University of Sydney, New South Wales, Australia.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2829254

Citation

Jiang, X M., et al. "A Low Copy Number Cosmid." Plasmid, vol. 18, no. 2, 1987, pp. 170-2.
Jiang XM, Brahmbhatt HN, Quigley NB, et al. A low copy number cosmid. Plasmid. 1987;18(2):170-2.
Jiang, X. M., Brahmbhatt, H. N., Quigley, N. B., & Reeves, P. R. (1987). A low copy number cosmid. Plasmid, 18(2), pp. 170-2.
Jiang XM, et al. A Low Copy Number Cosmid. Plasmid. 1987;18(2):170-2. PubMed PMID: 2829254.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A low copy number cosmid. AU - Jiang,X M, AU - Brahmbhatt,H N, AU - Quigley,N B, AU - Reeves,P R, PY - 1987/9/1/pubmed PY - 1987/9/1/medline PY - 1987/9/1/entrez SP - 170 EP - 2 JF - Plasmid JO - Plasmid VL - 18 IS - 2 N2 - A low copy number cosmid was constructed by subcloning the pair of cos sites and the kanamycin resistance gene of pcos2EMBL into pGB2. The resulting cosmid, pPR691, has the pSC101 replicon and specifies resistance to kanamycin, spectinomycin, and streptomycin. pPR691 also carries restriction sites suitable for cloning partial Sau3A digests using the strategy of Bates and Swift (P. F. Bates and R. A. Swift, 1983, Gene 26, 137-146). A library of Salmonella typhimurium chromosomal DNA was made using this cosmid and the rfb gene cluster (map position 42) was isolated from this library. SN - 0147-619X UR - https://www.unboundmedicine.com/medline/citation/2829254/A_low_copy_number_cosmid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0147-619X(87)90045-X DB - PRIME DP - Unbound Medicine ER -