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[Isolation of Junin virus from blood and peripheral lymphocytes of infected Calomys musculinus].
Rev Argent Microbiol. 1985; 17(3):177-81.RA

Abstract

Neonatal Calomys musculinus experimental infection with Junín virus (JV) XJCl3 strain causes either death or a persistent infection in the major part of surviving animals. JV can be isolated from peritoneal macrophages early during infection, and from brain and salivary glands during the chronic state of disease. It was of interest to investigate the appearance of virus in blood of infected animals. For this purpose, we decided to study the development of viremia in inoculated cricetids. A high frequency of viremia was registered during the acute state of disease (figure 1), which became sporadic approximately from 20 days post-infection onwards. Considering the results above mentioned, the characteristic lymphotropism of arenaviruses and the well-known JV replication in human mononuclear cells, cultures of lympho-monocytes obtained from blood of infected C. musculinus were done in order to investigate the eventual detection of infectious virus in the supernatants. JV was isolated, although in low titres, from cultures established with mononuclear cells belonging to animals in the acute state of disease (table 1); in the case of chronically infected cricetids, attempts to isolate JV were negative. These results show that viremia is currently detected in experimentally infected C. musculinus and that circulating mononuclear cells are permissive for JV multiplication, during the acute state of disease.

Authors+Show Affiliations

Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.No affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

spa

PubMed ID

2829277

Citation

Alche, L E., et al. "[Isolation of Junin Virus From Blood and Peripheral Lymphocytes of Infected Calomys Musculinus]." Revista Argentina De Microbiologia, vol. 17, no. 3, 1985, pp. 177-81.
Alche LE, Coulombie FC, Coto CE. [Isolation of Junin virus from blood and peripheral lymphocytes of infected Calomys musculinus]. Rev Argent Microbiol. 1985;17(3):177-81.
Alche, L. E., Coulombie, F. C., & Coto, C. E. (1985). [Isolation of Junin virus from blood and peripheral lymphocytes of infected Calomys musculinus]. Revista Argentina De Microbiologia, 17(3), 177-81.
Alche LE, Coulombie FC, Coto CE. [Isolation of Junin Virus From Blood and Peripheral Lymphocytes of Infected Calomys Musculinus]. Rev Argent Microbiol. 1985;17(3):177-81. PubMed PMID: 2829277.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Isolation of Junin virus from blood and peripheral lymphocytes of infected Calomys musculinus]. AU - Alche,L E, AU - Coulombie,F C, AU - Coto,C E, PY - 1985/1/1/pubmed PY - 1985/1/1/medline PY - 1985/1/1/entrez SP - 177 EP - 81 JF - Revista Argentina de microbiologia JO - Rev Argent Microbiol VL - 17 IS - 3 N2 - Neonatal Calomys musculinus experimental infection with Junín virus (JV) XJCl3 strain causes either death or a persistent infection in the major part of surviving animals. JV can be isolated from peritoneal macrophages early during infection, and from brain and salivary glands during the chronic state of disease. It was of interest to investigate the appearance of virus in blood of infected animals. For this purpose, we decided to study the development of viremia in inoculated cricetids. A high frequency of viremia was registered during the acute state of disease (figure 1), which became sporadic approximately from 20 days post-infection onwards. Considering the results above mentioned, the characteristic lymphotropism of arenaviruses and the well-known JV replication in human mononuclear cells, cultures of lympho-monocytes obtained from blood of infected C. musculinus were done in order to investigate the eventual detection of infectious virus in the supernatants. JV was isolated, although in low titres, from cultures established with mononuclear cells belonging to animals in the acute state of disease (table 1); in the case of chronically infected cricetids, attempts to isolate JV were negative. These results show that viremia is currently detected in experimentally infected C. musculinus and that circulating mononuclear cells are permissive for JV multiplication, during the acute state of disease. SN - 0325-7541 UR - https://www.unboundmedicine.com/medline/citation/2829277/[Isolation_of_Junin_virus_from_blood_and_peripheral_lymphocytes_of_infected_Calomys_musculinus]_ DB - PRIME DP - Unbound Medicine ER -