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Phytochemical Screening, Alpha-Glucosidase Inhibition, Antibacterial and Antioxidant Potential of Ajuga bracteosa Extracts.
Curr Pharm Biotechnol. 2017; 18(4):336-342.CP

Abstract

BACKGROUND

Ajuga bracteosa, a medicinal herb, is used by local community to cure a number of diseases such as inflammation, jaundice bronchial asthma, cancer and diabetes.

OBJECTIVES

The aim of present work was to evaluate the antioxidant potential, in vitro antidiabetic and antimicrobial effects of A. bracteosa.

METHODS

n-hexane, ethyl acetate, chloroform, acetone, methanol and aqueous extracts of Ajuga bracteosa roots, were prepared via maceration. Antibacterial activity was carried out by agar well diffusion method. Quantitative and qualitative phytochemical screening was done. The antioxidant activity was determined by iron (II) chelating activity, iron reducing power, DPPH, and ABTS free radical scavenging methods, Antidiabetic activity was evaluated through inhibition of α-glucosidase assay.

RESULTS

Phytochemical analysis showed the presence of phenols, flavonoids, tannins, saponins, quinines, terpenoids, xanthoproteins, glycosides, carbohydrates, steroids, phytosterols and amino acids. DPPH and ABTS potential values were recorded as 61.92% to 88.84% and 0.11% to 38.82%, respectively. Total phenolic and total flavonoid contents were expressed as gallic acid and rutin equivalents. Total iron content was expressed as FeSO4 equivalents. Chloroform and n-hexane extracts showed significant enzyme inhibition potential with IC50 values of 29.92 μg/ml and 131.7 μg/ml respectively. Aqueous extract showed maximum inhibition of E. coli, S. typhimurium, E. amnigenus, S. pyogenes, and S. aureus, (18.0±1.0 mm, 12.5±0.7 mm, 17.0±0.0 mm, 11.0±0.0 mm and 15.3±2.0 mm mm), respectively. Similarly, n-hexane extract showed maximum inhibition of E. coli, E. amnigenus, S. aureus (11.6±1.5 mm; 11.3±1.5 mm; 13.3±0.5 mm). This study also shows that n-hexane, chloroform, ethyl acetate and aqueous extracts of A. bracteosa root possess α-glucosidase inhibitory activities and therefore it may be used as hypoglycemic agents in the management of postprandial hyperglycemia.

CONCLUSION

Ajuga bracteosa root extracts may provide a basis for development of antioxidant, antimicrobial and antidiabetic drugs.

Authors+Show Affiliations

Department of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad. Pakistan.Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad, 13100. Pakistan.Department of Chemistry, Mirpur University of Science and Technology (MUST) Azad Jammu and Kashmir, Mirpur-10250. Pakistan.Microbial Biotechnology Laboratory, Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad. Pakistan.Microbial Biotechnology Laboratory, Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad. Pakistan.Microbial Biotechnology Laboratory, Department of Zoology, University of Azad Jammu and Kashmir, Muzaffarabad. Pakistan.Department of Chemistry, University of Azad Jammu and Kashmir, Muzaffarabad. Pakistan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28294059

Citation

Hafeez, Kokab, et al. "Phytochemical Screening, Alpha-Glucosidase Inhibition, Antibacterial and Antioxidant Potential of Ajuga Bracteosa Extracts." Current Pharmaceutical Biotechnology, vol. 18, no. 4, 2017, pp. 336-342.
Hafeez K, Andleeb S, Ghousa T, et al. Phytochemical Screening, Alpha-Glucosidase Inhibition, Antibacterial and Antioxidant Potential of Ajuga bracteosa Extracts. Curr Pharm Biotechnol. 2017;18(4):336-342.
Hafeez, K., Andleeb, S., Ghousa, T., Mustafa, R. G., Naseer, A., Shafique, I., & Akhter, K. (2017). Phytochemical Screening, Alpha-Glucosidase Inhibition, Antibacterial and Antioxidant Potential of Ajuga bracteosa Extracts. Current Pharmaceutical Biotechnology, 18(4), 336-342. https://doi.org/10.2174/1389201018666170313095033
Hafeez K, et al. Phytochemical Screening, Alpha-Glucosidase Inhibition, Antibacterial and Antioxidant Potential of Ajuga Bracteosa Extracts. Curr Pharm Biotechnol. 2017;18(4):336-342. PubMed PMID: 28294059.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemical Screening, Alpha-Glucosidase Inhibition, Antibacterial and Antioxidant Potential of Ajuga bracteosa Extracts. AU - Hafeez,Kokab, AU - Andleeb,Saiqa, AU - Ghousa,Tahseen, AU - Mustafa,Rozina G, AU - Naseer,Anum, AU - Shafique,Irsa, AU - Akhter,Kalsoom, PY - 2016/11/19/received PY - 2016/02/10/revised PY - 2017/02/28/accepted PY - 2017/3/16/pubmed PY - 2017/12/30/medline PY - 2017/3/16/entrez KW - Ajuga bracteosa KW - Alpha-glucosidase inhibition assay KW - antibacterial activity KW - antioxidant activity KW - phytochemical screening SP - 336 EP - 342 JF - Current pharmaceutical biotechnology JO - Curr Pharm Biotechnol VL - 18 IS - 4 N2 - BACKGROUND: Ajuga bracteosa, a medicinal herb, is used by local community to cure a number of diseases such as inflammation, jaundice bronchial asthma, cancer and diabetes. OBJECTIVES: The aim of present work was to evaluate the antioxidant potential, in vitro antidiabetic and antimicrobial effects of A. bracteosa. METHODS: n-hexane, ethyl acetate, chloroform, acetone, methanol and aqueous extracts of Ajuga bracteosa roots, were prepared via maceration. Antibacterial activity was carried out by agar well diffusion method. Quantitative and qualitative phytochemical screening was done. The antioxidant activity was determined by iron (II) chelating activity, iron reducing power, DPPH, and ABTS free radical scavenging methods, Antidiabetic activity was evaluated through inhibition of α-glucosidase assay. RESULTS: Phytochemical analysis showed the presence of phenols, flavonoids, tannins, saponins, quinines, terpenoids, xanthoproteins, glycosides, carbohydrates, steroids, phytosterols and amino acids. DPPH and ABTS potential values were recorded as 61.92% to 88.84% and 0.11% to 38.82%, respectively. Total phenolic and total flavonoid contents were expressed as gallic acid and rutin equivalents. Total iron content was expressed as FeSO4 equivalents. Chloroform and n-hexane extracts showed significant enzyme inhibition potential with IC50 values of 29.92 μg/ml and 131.7 μg/ml respectively. Aqueous extract showed maximum inhibition of E. coli, S. typhimurium, E. amnigenus, S. pyogenes, and S. aureus, (18.0±1.0 mm, 12.5±0.7 mm, 17.0±0.0 mm, 11.0±0.0 mm and 15.3±2.0 mm mm), respectively. Similarly, n-hexane extract showed maximum inhibition of E. coli, E. amnigenus, S. aureus (11.6±1.5 mm; 11.3±1.5 mm; 13.3±0.5 mm). This study also shows that n-hexane, chloroform, ethyl acetate and aqueous extracts of A. bracteosa root possess α-glucosidase inhibitory activities and therefore it may be used as hypoglycemic agents in the management of postprandial hyperglycemia. CONCLUSION: Ajuga bracteosa root extracts may provide a basis for development of antioxidant, antimicrobial and antidiabetic drugs. SN - 1873-4316 UR - https://www.unboundmedicine.com/medline/citation/28294059/Phytochemical_Screening_Alpha_Glucosidase_Inhibition_Antibacterial_and_Antioxidant_Potential_of_Ajuga_bracteosa_Extracts_ L2 - https://www.eurekaselect.com/150835/article DB - PRIME DP - Unbound Medicine ER -