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Hypocholesterolemic Effects of Probiotic Mixture on Diet-Induced Hypercholesterolemic Rats.

Abstract

Growing evidence has indicated that supplementation with probiotics improves lipid metabolism. We aimed to investigate the beneficial effects of a probiotics mixture (PM) of three strains belonging to the species Bifidobacterium (B. longum, B. lactis, and B. breve) and two strains belonging to the species Lactobacillus (L. reuteri and L. plantarum) on cholesterol-lowering efficacy in hypercholesterolemic rats. A hypercholesterolemic rat model was established by feeding a high-cholesterol diet for eight weeks. To test the effects of PM on hypercholesterolemia, hypercholesterolemic rats were assigned to four groups, which were treated daily with low (1.65 × 10⁸ cfu/kg), medium (5.5 × 10⁸ cfu/kg), or high (1.65 × 1010 cfu/kg) doses of probiotic mixture or simvastatin for eight weeks. Significant reductions of serum total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein (LDL)-cholesterol levels, but increases of high-density lipoprotein (HDL)-cholesterol were observed after supplementation of PM in hypercholesterolemic rats. In PM-supplemented hypercholesterolemic rats, hepatic tissue contents of TC and TG also significantly decreased. Notably, the histological evaluation of liver tissues demonstrated that PM dramatically decreased lipid accumulation. For their underlying mechanisms, we demonstrated that PM reduced expressions of cholesterol synthesis-related proteins such as sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in the liver. Taken together, these findings suggest that PM has beneficial effects against hypercholesterolemia. Accordingly, our PM might be utilized as a novel therapeutic agent for the management of hypercholesterolemia.

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  • Authors+Show Affiliations

    ,

    Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Biosafety Research Institute and Korea Zoonosis Research Institue, Chonbuk National University, Iksan 54596, Korea. abbasj@jbnu.ac.kr.

    ,

    Huvet Co. Ltd., Iksan 54541, Korea. hyunmu123@hanmail.net.

    ,

    Chebigen Co. Ltd., Jeonju 54853, Korea. shsdo@hanmail.net.

    ,

    Chebigen Co. Ltd., Jeonju 54853, Korea. sampo999@hanmail.net.

    ,

    Chebigen Co. Ltd., Jeonju 54853, Korea. cbg31@chebigen.com.

    ,

    Jeonju Agro-Biomaterials Institute, Jeonju 54810, Korea. seon02@jami.re.kr.

    ,

    Jeonju Agro-Biomaterials Institute, Jeonju 54810, Korea. jasmin0207@jami.re.kr.

    ,

    Jeonju Agro-Biomaterials Institute, Jeonju 54810, Korea. kangyu@jami.re.kr.

    ,

    Jeonju Agro-Biomaterials Institute, Jeonju 54810, Korea. zcbmmnn@jami.re.kr.

    Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Biosafety Research Institute and Korea Zoonosis Research Institue, Chonbuk National University, Iksan 54596, Korea. dkyang0502@gmail.com.

    Source

    Nutrients 9:3 2017 Mar 16 pg

    MeSH

    Acetyl-CoA Carboxylase
    Alanine Transaminase
    Animals
    Aspartate Aminotransferases
    Bifidobacterium
    Cholesterol, HDL
    Cholesterol, LDL
    Diet
    Fatty Acid Synthases
    Hypercholesterolemia
    Lactobacillus
    Liver
    Male
    Probiotics
    Rats
    Rats, Sprague-Dawley
    Sterol Regulatory Element Binding Protein 1
    Triglycerides

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    28300786

    Citation

    Kim, Shang-Jin, et al. "Hypocholesterolemic Effects of Probiotic Mixture On Diet-Induced Hypercholesterolemic Rats." Nutrients, vol. 9, no. 3, 2017.
    Kim SJ, Park SH, Sin HS, et al. Hypocholesterolemic Effects of Probiotic Mixture on Diet-Induced Hypercholesterolemic Rats. Nutrients. 2017;9(3).
    Kim, S. J., Park, S. H., Sin, H. S., Jang, S. H., Lee, S. W., Kim, S. Y., ... Yang, D. K. (2017). Hypocholesterolemic Effects of Probiotic Mixture on Diet-Induced Hypercholesterolemic Rats. Nutrients, 9(3), doi:10.3390/nu9030293.
    Kim SJ, et al. Hypocholesterolemic Effects of Probiotic Mixture On Diet-Induced Hypercholesterolemic Rats. Nutrients. 2017 Mar 16;9(3) PubMed PMID: 28300786.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Hypocholesterolemic Effects of Probiotic Mixture on Diet-Induced Hypercholesterolemic Rats. AU - Kim,Shang-Jin, AU - Park,Sang Hoon, AU - Sin,Hong-Sig, AU - Jang,Seung-Hwan, AU - Lee,Sang-Wang, AU - Kim,Seon-Young, AU - Kwon,Bora, AU - Yu,Kang-Yeol, AU - Kim,Su Young, AU - Yang,Dong Kwon, Y1 - 2017/03/16/ PY - 2017/01/09/received PY - 2017/03/08/revised PY - 2017/03/10/accepted PY - 2017/3/17/entrez PY - 2017/3/17/pubmed PY - 2017/9/15/medline KW - Bifidobacterium breve KW - Bifidobacterium lactis KW - Bifidobacterium longum KW - Lactobacillus plantarum KW - Lactobacillus reuteri KW - high-cholesterol diet KW - hypercholesterolemia KW - liver steatosis KW - probiotics mixture JF - Nutrients JO - Nutrients VL - 9 IS - 3 N2 - Growing evidence has indicated that supplementation with probiotics improves lipid metabolism. We aimed to investigate the beneficial effects of a probiotics mixture (PM) of three strains belonging to the species Bifidobacterium (B. longum, B. lactis, and B. breve) and two strains belonging to the species Lactobacillus (L. reuteri and L. plantarum) on cholesterol-lowering efficacy in hypercholesterolemic rats. A hypercholesterolemic rat model was established by feeding a high-cholesterol diet for eight weeks. To test the effects of PM on hypercholesterolemia, hypercholesterolemic rats were assigned to four groups, which were treated daily with low (1.65 × 10⁸ cfu/kg), medium (5.5 × 10⁸ cfu/kg), or high (1.65 × 1010 cfu/kg) doses of probiotic mixture or simvastatin for eight weeks. Significant reductions of serum total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein (LDL)-cholesterol levels, but increases of high-density lipoprotein (HDL)-cholesterol were observed after supplementation of PM in hypercholesterolemic rats. In PM-supplemented hypercholesterolemic rats, hepatic tissue contents of TC and TG also significantly decreased. Notably, the histological evaluation of liver tissues demonstrated that PM dramatically decreased lipid accumulation. For their underlying mechanisms, we demonstrated that PM reduced expressions of cholesterol synthesis-related proteins such as sterol regulatory element-binding protein 1 (SREBP1), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in the liver. Taken together, these findings suggest that PM has beneficial effects against hypercholesterolemia. Accordingly, our PM might be utilized as a novel therapeutic agent for the management of hypercholesterolemia. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/28300786/Hypocholesterolemic_Effects_of_Probiotic_Mixture_on_Diet_Induced_Hypercholesterolemic_Rats_ L2 - http://www.mdpi.com/resolver?pii=nu9030293 DB - PRIME DP - Unbound Medicine ER -