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Ameliorative effect of gossypin against gentamicin-induced nephrotoxicity in rats.
Life Sci. 2017 May 01; 176:75-81.LS

Abstract

AIM

Gentamicin (GEN) is an aminoglycoside antibiotic employed in treatment of life-threatening gram-negative infections, but one of its major side effects is the induction of nephrotoxicity. Therefore, the aim of this work was to scrutinize the possible protective effect of gossypin against GEN-induced nephrotoxicity.

METHOD

Rats were randomly divided into four groups. First group served as a control, second group was injected with gossypin (10mg/kg, orally) for 7days, third group was injected with GEN (80mg/kg, i.p.) and the fourth group was co-treated with GEN and gossypin for 7days.

KEY FINDING

GEN-treated group showed kidney dysfunction as proteinuria excretion rate, podocalyxin excretion rates, renal monocyte chemoattractant protein-1 (MCP-1), blood urea nitrogen (BUN) and plasma creatinine were significantly increased as well as tubular degeneration occur. The significant decrease in renal reduced glutathione (GSH) level, superoxide dismutase (SOD) and catalase (CAT) activities and an increase in thiobarbituric acid reactive substances (TBARs) level, indicated that GEN-induced nephrotoxicity through oxidative stress reactions. Also, GEN up-regulated both gene expression and renal levels of inflammatory cytokines TNF-α and IL-6. On the other hand, concurrent treatment of gossypin plus GEN protected kidney tissues against nephrotoxic effects of GEN through elevated levels of renal GSH, SOD and CAT activities while decreased in renal TBARs level. In addition, gossypin down-regulated gene expression and renal levels of inflammatory cytokines TNF-α and IL-6 induced by GEN.

SIGNIFICANCE

This study revealed that gossypin exerts protection against nephrotoxicity induced by gentamicin via its antioxidant activity.

Authors+Show Affiliations

Pharmacology and Toxicology Department, Faculty of Pharmacy, Damanhour University, Egypt.Biochemistry Department, Faculty of Pharmacy, Damanhour University, Egypt. Electronic address: salahuddin@pharm.dmu.edu.eg.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28302561

Citation

Katary, Mohamed, and Ahmad Salahuddin. "Ameliorative Effect of Gossypin Against Gentamicin-induced Nephrotoxicity in Rats." Life Sciences, vol. 176, 2017, pp. 75-81.
Katary M, Salahuddin A. Ameliorative effect of gossypin against gentamicin-induced nephrotoxicity in rats. Life Sci. 2017;176:75-81.
Katary, M., & Salahuddin, A. (2017). Ameliorative effect of gossypin against gentamicin-induced nephrotoxicity in rats. Life Sciences, 176, 75-81. https://doi.org/10.1016/j.lfs.2017.03.009
Katary M, Salahuddin A. Ameliorative Effect of Gossypin Against Gentamicin-induced Nephrotoxicity in Rats. Life Sci. 2017 May 1;176:75-81. PubMed PMID: 28302561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorative effect of gossypin against gentamicin-induced nephrotoxicity in rats. AU - Katary,Mohamed, AU - Salahuddin,Ahmad, Y1 - 2017/03/14/ PY - 2016/12/29/received PY - 2017/03/08/revised PY - 2017/03/12/accepted PY - 2017/3/18/pubmed PY - 2017/4/30/medline PY - 2017/3/18/entrez KW - Antioxidant KW - Gentamicin KW - Gossypin KW - IL-6 KW - Monocyte chemoattractant protein-1 KW - Nephrotoxicity KW - TNF-α SP - 75 EP - 81 JF - Life sciences JO - Life Sci VL - 176 N2 - AIM: Gentamicin (GEN) is an aminoglycoside antibiotic employed in treatment of life-threatening gram-negative infections, but one of its major side effects is the induction of nephrotoxicity. Therefore, the aim of this work was to scrutinize the possible protective effect of gossypin against GEN-induced nephrotoxicity. METHOD: Rats were randomly divided into four groups. First group served as a control, second group was injected with gossypin (10mg/kg, orally) for 7days, third group was injected with GEN (80mg/kg, i.p.) and the fourth group was co-treated with GEN and gossypin for 7days. KEY FINDING: GEN-treated group showed kidney dysfunction as proteinuria excretion rate, podocalyxin excretion rates, renal monocyte chemoattractant protein-1 (MCP-1), blood urea nitrogen (BUN) and plasma creatinine were significantly increased as well as tubular degeneration occur. The significant decrease in renal reduced glutathione (GSH) level, superoxide dismutase (SOD) and catalase (CAT) activities and an increase in thiobarbituric acid reactive substances (TBARs) level, indicated that GEN-induced nephrotoxicity through oxidative stress reactions. Also, GEN up-regulated both gene expression and renal levels of inflammatory cytokines TNF-α and IL-6. On the other hand, concurrent treatment of gossypin plus GEN protected kidney tissues against nephrotoxic effects of GEN through elevated levels of renal GSH, SOD and CAT activities while decreased in renal TBARs level. In addition, gossypin down-regulated gene expression and renal levels of inflammatory cytokines TNF-α and IL-6 induced by GEN. SIGNIFICANCE: This study revealed that gossypin exerts protection against nephrotoxicity induced by gentamicin via its antioxidant activity. SN - 1879-0631 UR - https://www.unboundmedicine.com/medline/citation/28302561/Ameliorative_effect_of_gossypin_against_gentamicin_induced_nephrotoxicity_in_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0024-3205(17)30092-9 DB - PRIME DP - Unbound Medicine ER -