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Purification, properties, and immunochemical localization of a receptor for intrinsic factor-cobalamin complex in the rat kidney.
J Biol Chem. 1988 Mar 25; 263(9):4443-9.JB

Abstract

High levels of receptor for intrinsic factor-cobalamin (vitamin B12) were detected in human, canine, and rat kidneys. The ratio of specific activity (picomoles/mg of protein) for kidney relative to intestine was 116, 20, and 797, respectively, in these species. The receptor was purified about 3000-fold from 200 g of rat kidney with a recovery of 16% and exhibited a single band on nondenaturing gel electrophoresis. Quantitative amino acid analysis of the receptor gave a value of 457,310 g of amino acid/mol of intrinsic factor-cobalamin binding activity. The pure receptor revealed an Mr of 430,000, as assessed by filtration with Bio-Gel A-5m. Treatment with papain resulted in the production of active monomers of Mr to about 205,000-210,000. Electrophoresis in the presence of sodium dodecyl sulfate confirmed the monomer Mr to be 230,000. The monomer receptor did not reveal the presence of any further subunits upon reductive alkylation. Following cyanogen bromide cleavage the kidney receptor revealed three peptides of Mr 115,000, 60,000, and 54,000. The pI of these peptides was 5.17, 6.17, and 6.17, respectively. Western blot analysis using antiserum raised to the receptor demonstrated a protein with an Mr of 175,000 and 230,000 for intestinal and kidney membrane receptors, respectively. Immunologically, the rat kidney receptor was identical to the rat ileal receptor but was distinct from the canine ileal receptor. Ultrastructural localization revealed the presence of the receptor in the apical surface membrane of proximal tubular cells of the kidney and absorptive cells of the ileum. The kidney is the best source for obtaining this receptor in reasonable quantities.

Authors+Show Affiliations

Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2831230

Citation

Seetharam, B, et al. "Purification, Properties, and Immunochemical Localization of a Receptor for Intrinsic Factor-cobalamin Complex in the Rat Kidney." The Journal of Biological Chemistry, vol. 263, no. 9, 1988, pp. 4443-9.
Seetharam B, Levine JS, Ramasamy M, et al. Purification, properties, and immunochemical localization of a receptor for intrinsic factor-cobalamin complex in the rat kidney. J Biol Chem. 1988;263(9):4443-9.
Seetharam, B., Levine, J. S., Ramasamy, M., & Alpers, D. H. (1988). Purification, properties, and immunochemical localization of a receptor for intrinsic factor-cobalamin complex in the rat kidney. The Journal of Biological Chemistry, 263(9), 4443-9.
Seetharam B, et al. Purification, Properties, and Immunochemical Localization of a Receptor for Intrinsic Factor-cobalamin Complex in the Rat Kidney. J Biol Chem. 1988 Mar 25;263(9):4443-9. PubMed PMID: 2831230.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification, properties, and immunochemical localization of a receptor for intrinsic factor-cobalamin complex in the rat kidney. AU - Seetharam,B, AU - Levine,J S, AU - Ramasamy,M, AU - Alpers,D H, PY - 1988/3/25/pubmed PY - 1988/3/25/medline PY - 1988/3/25/entrez SP - 4443 EP - 9 JF - The Journal of biological chemistry JO - J Biol Chem VL - 263 IS - 9 N2 - High levels of receptor for intrinsic factor-cobalamin (vitamin B12) were detected in human, canine, and rat kidneys. The ratio of specific activity (picomoles/mg of protein) for kidney relative to intestine was 116, 20, and 797, respectively, in these species. The receptor was purified about 3000-fold from 200 g of rat kidney with a recovery of 16% and exhibited a single band on nondenaturing gel electrophoresis. Quantitative amino acid analysis of the receptor gave a value of 457,310 g of amino acid/mol of intrinsic factor-cobalamin binding activity. The pure receptor revealed an Mr of 430,000, as assessed by filtration with Bio-Gel A-5m. Treatment with papain resulted in the production of active monomers of Mr to about 205,000-210,000. Electrophoresis in the presence of sodium dodecyl sulfate confirmed the monomer Mr to be 230,000. The monomer receptor did not reveal the presence of any further subunits upon reductive alkylation. Following cyanogen bromide cleavage the kidney receptor revealed three peptides of Mr 115,000, 60,000, and 54,000. The pI of these peptides was 5.17, 6.17, and 6.17, respectively. Western blot analysis using antiserum raised to the receptor demonstrated a protein with an Mr of 175,000 and 230,000 for intestinal and kidney membrane receptors, respectively. Immunologically, the rat kidney receptor was identical to the rat ileal receptor but was distinct from the canine ileal receptor. Ultrastructural localization revealed the presence of the receptor in the apical surface membrane of proximal tubular cells of the kidney and absorptive cells of the ileum. The kidney is the best source for obtaining this receptor in reasonable quantities. SN - 0021-9258 UR - https://www.unboundmedicine.com/medline/citation/2831230/Purification_properties_and_immunochemical_localization_of_a_receptor_for_intrinsic_factor_cobalamin_complex_in_the_rat_kidney_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9258(18)68946-X DB - PRIME DP - Unbound Medicine ER -