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Purification of foamy viral particles.

Abstract

Foamy viruses are non-pathogenic retroviruses and represent a tool for vector development. For gene therapy applications and for analyses of viral protein composition infectious particles need to be purified, which has been difficult for foamy viruses in the past. Here, we describe a novel, simple, and fast purification method for prototype foamy viruses with high purity using size exclusion and affinity chromatography. More than 99,9% of the contaminating proteins were removed. The purified viruses were used to determine the amount of the incorporated Pol protein relative to Gag. The determined Gag to Pol PR-RT ratio of 30:1 confirmed previous studies suggesting FV virions encapsidate fewer number of Pol molecules than orthoretroviruses.

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  • Authors+Show Affiliations

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    Institut für Virologie und Immunbiologie, Julius-Maximilians-Universität Würzburg, Germany.

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    Institut für Virologie und Immunbiologie, Julius-Maximilians-Universität Würzburg, Germany.

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    Institut für Virologie, Technische Universität Dresden, Germany.

    Institut für Virologie und Immunbiologie, Julius-Maximilians-Universität Würzburg, Germany. Electronic address: Jochen.Bodem@vim.uni-wuerzburg.de.

    Source

    Virology 506: 2017 Jun pg 28-33

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    28314126