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Responses to silver nanoparticles and silver nitrate in a battery of biomarkers measured in coelomocytes and in target tissues of Eisenia fetida earthworms.
Ecotoxicol Environ Saf. 2017 Jul; 141:57-63.EE

Abstract

The current use and development of applications with silver nanoparticles (Ag NPs) could lead to potential inputs of these NPs to soils. Consequently, it is crucial to understand the ecotoxicological risks posed by Ag NPs in the terrestrial compartment. In the present investigation, the effects produced by PVP-PEI coated Ag NPs were assessed in Eisenia fetida earthworms in comparison with the soluble form (AgNO3). Earthworms were exposed for 1, 3 and 14 days to a range of sublethal concentrations of Ag (0, 0.05 and 50mg/kg) and at each exposure time, apart from mortality and weight loss of individuals, metallothionein (MT) protein concentration and catalase (CAT) activity were quantified in earthworm tissues. In addition, cellular and molecular level endpoints (cell viability, absolute and relative trophic indices and transcription levels of catalase-cat- and metallothionein-mt-) were measured in coelomocytes extruded from exposed earthworms. Despite the lack of effects in traditional endpoints (mortality and weight loss), Ag NPs and AgNO3 posed changes at lower levels of biological complexity (biochemical, cellular and molecular levels). Both Ag forms induced similar changes in the metal detoxification mechanism (MT, mt) and in the antioxidant response system (CAT, cat) of E. fetida. In contrast, Ag form dependant cytotoxicity and subpopulation ratio alterations (eleocytes/amoebocytes) were recorded in extruded coelomocytes. Complementarily, the use of coelomocytes to assess molecular level endpoints represented a relevant alternative for development of non-invasive biomarkers.

Authors+Show Affiliations

National Council of Scientific and Technical Researches CONICET, Av. Rivadavia 1917, 1023 Buenos Aires, Argentina; Ecotoxicology Research Program, Department of Basic Sciences, National University of Luján, P.O. Box 221, B6700 Luján, Buenos Aires, Argentina.Cell Biology in Environmental Toxicology Research Group, Research Centre for Experimental Marine Biology and Biotechnology, and Zoology and Animal Cell Biology Department, Faculty of Science & Technology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Basque Country, Spain.Cell Biology in Environmental Toxicology Research Group, Research Centre for Experimental Marine Biology and Biotechnology, and Zoology and Animal Cell Biology Department, Faculty of Science & Technology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Basque Country, Spain.National Council of Scientific and Technical Researches CONICET, Av. Rivadavia 1917, 1023 Buenos Aires, Argentina; Ecotoxicology Research Program, Department of Basic Sciences, National University of Luján, P.O. Box 221, B6700 Luján, Buenos Aires, Argentina.Cell Biology in Environmental Toxicology Research Group, Research Centre for Experimental Marine Biology and Biotechnology, and Zoology and Animal Cell Biology Department, Faculty of Science & Technology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Basque Country, Spain.National Council of Scientific and Technical Researches CONICET, Av. Rivadavia 1917, 1023 Buenos Aires, Argentina; Ecotoxicology Research Program, Department of Basic Sciences, National University of Luján, P.O. Box 221, B6700 Luján, Buenos Aires, Argentina.Cell Biology in Environmental Toxicology Research Group, Research Centre for Experimental Marine Biology and Biotechnology, and Zoology and Animal Cell Biology Department, Faculty of Science & Technology, University of the Basque Country, P.O. Box 644, 48080 Bilbao, Basque Country, Spain. Electronic address: manu.soto@ehu.eus.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28314142

Citation

Patricia, Curieses Silvana, et al. "Responses to Silver Nanoparticles and Silver Nitrate in a Battery of Biomarkers Measured in Coelomocytes and in Target Tissues of Eisenia Fetida Earthworms." Ecotoxicology and Environmental Safety, vol. 141, 2017, pp. 57-63.
Patricia CS, Nerea GV, Erik U, et al. Responses to silver nanoparticles and silver nitrate in a battery of biomarkers measured in coelomocytes and in target tissues of Eisenia fetida earthworms. Ecotoxicol Environ Saf. 2017;141:57-63.
Patricia, C. S., Nerea, G. V., Erik, U., Elena, S. M., Eider, B., Darío, D. M. W., & Manu, S. (2017). Responses to silver nanoparticles and silver nitrate in a battery of biomarkers measured in coelomocytes and in target tissues of Eisenia fetida earthworms. Ecotoxicology and Environmental Safety, 141, 57-63. https://doi.org/10.1016/j.ecoenv.2017.03.008
Patricia CS, et al. Responses to Silver Nanoparticles and Silver Nitrate in a Battery of Biomarkers Measured in Coelomocytes and in Target Tissues of Eisenia Fetida Earthworms. Ecotoxicol Environ Saf. 2017;141:57-63. PubMed PMID: 28314142.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Responses to silver nanoparticles and silver nitrate in a battery of biomarkers measured in coelomocytes and in target tissues of Eisenia fetida earthworms. AU - Patricia,Curieses Silvana, AU - Nerea,García-Velasco, AU - Erik,Urionabarrenetxea, AU - Elena,Sáenz María, AU - Eider,Bilbao, AU - Darío,Di Marzio Walter, AU - Manu,Soto, Y1 - 2017/03/19/ PY - 2016/10/31/received PY - 2017/03/02/revised PY - 2017/03/07/accepted PY - 2017/3/18/pubmed PY - 2017/7/25/medline PY - 2017/3/18/entrez KW - Antioxidant response KW - Coelomocyte cytotoxicity KW - Eisenia fetida KW - Metal detoxification KW - Silver nanoparticle KW - Soil ecotoxicology SP - 57 EP - 63 JF - Ecotoxicology and environmental safety JO - Ecotoxicol Environ Saf VL - 141 N2 - The current use and development of applications with silver nanoparticles (Ag NPs) could lead to potential inputs of these NPs to soils. Consequently, it is crucial to understand the ecotoxicological risks posed by Ag NPs in the terrestrial compartment. In the present investigation, the effects produced by PVP-PEI coated Ag NPs were assessed in Eisenia fetida earthworms in comparison with the soluble form (AgNO3). Earthworms were exposed for 1, 3 and 14 days to a range of sublethal concentrations of Ag (0, 0.05 and 50mg/kg) and at each exposure time, apart from mortality and weight loss of individuals, metallothionein (MT) protein concentration and catalase (CAT) activity were quantified in earthworm tissues. In addition, cellular and molecular level endpoints (cell viability, absolute and relative trophic indices and transcription levels of catalase-cat- and metallothionein-mt-) were measured in coelomocytes extruded from exposed earthworms. Despite the lack of effects in traditional endpoints (mortality and weight loss), Ag NPs and AgNO3 posed changes at lower levels of biological complexity (biochemical, cellular and molecular levels). Both Ag forms induced similar changes in the metal detoxification mechanism (MT, mt) and in the antioxidant response system (CAT, cat) of E. fetida. In contrast, Ag form dependant cytotoxicity and subpopulation ratio alterations (eleocytes/amoebocytes) were recorded in extruded coelomocytes. Complementarily, the use of coelomocytes to assess molecular level endpoints represented a relevant alternative for development of non-invasive biomarkers. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/28314142/Responses_to_silver_nanoparticles_and_silver_nitrate_in_a_battery_of_biomarkers_measured_in_coelomocytes_and_in_target_tissues_of_Eisenia_fetida_earthworms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(17)30140-9 DB - PRIME DP - Unbound Medicine ER -