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DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice.
Vaccine. 2017 04 11; 35(16):2069-2075.V

Abstract

The Middle East respiratory syndrome coronavirus (MERS-CoV), is an emerging pathogen that continues to cause outbreaks in the Arabian peninsula and in travelers from this region, raising the concern that a global pandemic could occur. Here, we show that a DNA vaccine encoding the first 725 amino acids (S1) of MERS-CoV spike (S) protein induces antigen-specific humoral and cellular immune responses in mice. With three immunizations, high titers of neutralizing antibodies (up to 1: 104) were generated without adjuvant. DNA vaccination with the MERS-CoV S1 gene markedly increased the frequencies of antigen-specific CD4+ and CD8+ T cells secreting IFN-γ and other cytokines. Both pcDNA3.1-S1 DNA vaccine immunization and passive transfer of immune serum from pcDNA3.1-S1 vaccinated mice protected Ad5-hDPP4-transduced mice from MERS-CoV challenge. These results demonstrate that a DNA vaccine encoding MERS-CoV S1 protein induces strong protective immune responses against MERS-CoV infection.

Authors+Show Affiliations

Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China; School of Public Health, Shandong University, Jinan, China.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China.Department of Microbiology, University of Iowa, Iowa City, IA, USA.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China. Electronic address: yst62041@163.com.State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China. Electronic address: zhaojincun@gird.cn.Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary, Academy of Military Medical Science, Changchun, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China. Electronic address: xiaxzh@cae.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28314561

Citation

Chi, Hang, et al. "DNA Vaccine Encoding Middle East Respiratory Syndrome Coronavirus S1 Protein Induces Protective Immune Responses in Mice." Vaccine, vol. 35, no. 16, 2017, pp. 2069-2075.
Chi H, Zheng X, Wang X, et al. DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice. Vaccine. 2017;35(16):2069-2075.
Chi, H., Zheng, X., Wang, X., Wang, C., Wang, H., Gai, W., Perlman, S., Yang, S., Zhao, J., & Xia, X. (2017). DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice. Vaccine, 35(16), 2069-2075. https://doi.org/10.1016/j.vaccine.2017.02.063
Chi H, et al. DNA Vaccine Encoding Middle East Respiratory Syndrome Coronavirus S1 Protein Induces Protective Immune Responses in Mice. Vaccine. 2017 04 11;35(16):2069-2075. PubMed PMID: 28314561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice. AU - Chi,Hang, AU - Zheng,Xuexing, AU - Wang,Xiwen, AU - Wang,Chong, AU - Wang,Hualei, AU - Gai,Weiwei, AU - Perlman,Stanley, AU - Yang,Songtao, AU - Zhao,Jincun, AU - Xia,Xianzhu, Y1 - 2017/03/14/ PY - 2016/06/10/received PY - 2017/02/13/revised PY - 2017/02/28/accepted PY - 2017/3/21/pubmed PY - 2017/12/20/medline PY - 2017/3/19/entrez KW - DNA vaccine KW - MERS-CoV KW - Spike protein SP - 2069 EP - 2075 JF - Vaccine JO - Vaccine VL - 35 IS - 16 N2 - The Middle East respiratory syndrome coronavirus (MERS-CoV), is an emerging pathogen that continues to cause outbreaks in the Arabian peninsula and in travelers from this region, raising the concern that a global pandemic could occur. Here, we show that a DNA vaccine encoding the first 725 amino acids (S1) of MERS-CoV spike (S) protein induces antigen-specific humoral and cellular immune responses in mice. With three immunizations, high titers of neutralizing antibodies (up to 1: 104) were generated without adjuvant. DNA vaccination with the MERS-CoV S1 gene markedly increased the frequencies of antigen-specific CD4+ and CD8+ T cells secreting IFN-γ and other cytokines. Both pcDNA3.1-S1 DNA vaccine immunization and passive transfer of immune serum from pcDNA3.1-S1 vaccinated mice protected Ad5-hDPP4-transduced mice from MERS-CoV challenge. These results demonstrate that a DNA vaccine encoding MERS-CoV S1 protein induces strong protective immune responses against MERS-CoV infection. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/28314561/DNA_vaccine_encoding_Middle_East_respiratory_syndrome_coronavirus_S1_protein_induces_protective_immune_responses_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(17)30288-8 DB - PRIME DP - Unbound Medicine ER -