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Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats.
Pharm Biol 2017; 55(1):1389-1393PB

Abstract

CONTEXT

Stachys pilifera Benth (Lamiaceae) has long been used to treat infectious diseases, respiratory and rheumatoid disorders in Iranian folk medicine. Antitumor and antioxidant activity of the plant have been reported.

OBJECTIVE

The study was designed to assess the hepatoprotective activity of ethanol extract of Stachys pilifera in carbon tetrachloride (CCl4)-induced hepatotoxicity in rats.

MATERIALS AND METHODS

The rats were randomly divided into six equal groups (n = 7). Group I was treated with normal saline; Group II received CCl4 (1 mL/kg. i.p., twice a week) for 60 consecutive days; Groups III, IV and V were given CCl4 plus Stachys pilifera (100, 200 and 400 mg/kg/d,p.o.); Group VI received the extract (400 mg/kg/d, p.o.). Histopathological analysis and measurement of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), malondialdehyde (MDA), total protein (TP) and albumin (ALB) were performed.

RESULTS

CCl4 caused a significant increase in the serum levels of AST, ALT, ALP and MDA as well as decreased ALB, and TP serum levels (p < 0.001). The extract (200 and 400 mg/kg/d) significantly normalized the CCl4-elevated levels of ALT, AST, ALP and MDA (p < 0.001). The extract (200 and 400 mg/kg/d) also increased the serum levels of TP compared to CCl4 group (p< 0.01). The extract (200 and 400 mg/kg/d) also decreased the histological injuries (inflammation and fatty degeneration) by CCl4.

DISCUSSION

The results revealed that the Stachys pilifera extract could provide considerable protection against CCl4 hepatotoxicity in rats that may be related to its antioxidant properties.

Authors+Show Affiliations

a Department of Biology, Faculty of Science , Guilan University , Rasht , Iran.b Biochemistry Department, Faculty of Medicine , AJA University of Medical Sciences , Tehran , Iran.c Medicinal Plants Research Center , Yasuj University of Medical Sciences , Yasuj , Iran.d Cellular and Molecular Research Center , Yasuj University of Medical Sciences , Yasuj , Iran.e School of Nursing, AJA University of Medical Sciences , Tehran , Iran.c Medicinal Plants Research Center , Yasuj University of Medical Sciences , Yasuj , Iran.a Department of Biology, Faculty of Science , Guilan University , Rasht , Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28317417

Citation

Panahi Kokhdan, Esmaeel, et al. "Hepatoprotective Effect of Stachys Pilifera Ethanol Extract in Carbon Tetrachloride-induce Hepatotoxicity in Rats." Pharmaceutical Biology, vol. 55, no. 1, 2017, pp. 1389-1393.
Panahi Kokhdan E, Ahmadi K, Sadeghi H, et al. Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats. Pharm Biol. 2017;55(1):1389-1393.
Panahi Kokhdan, E., Ahmadi, K., Sadeghi, H., Sadeghi, H., Dadgary, F., Danaei, N., & Aghamaali, M. R. (2017). Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats. Pharmaceutical Biology, 55(1), pp. 1389-1393. doi:10.1080/13880209.2017.1302484.
Panahi Kokhdan E, et al. Hepatoprotective Effect of Stachys Pilifera Ethanol Extract in Carbon Tetrachloride-induce Hepatotoxicity in Rats. Pharm Biol. 2017;55(1):1389-1393. PubMed PMID: 28317417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective effect of Stachys pilifera ethanol extract in carbon tetrachloride-induce hepatotoxicity in rats. AU - Panahi Kokhdan,Esmaeel, AU - Ahmadi,Kyomarth, AU - Sadeghi,Heibatollah, AU - Sadeghi,Hossein, AU - Dadgary,Fahemeh, AU - Danaei,Nazanin, AU - Aghamaali,Mahmoud Reza, PY - 2017/3/21/entrez PY - 2017/3/21/pubmed PY - 2017/6/6/medline KW - ALP KW - AST KW - MDA KW - hepatotoxicity SP - 1389 EP - 1393 JF - Pharmaceutical biology JO - Pharm Biol VL - 55 IS - 1 N2 - CONTEXT: Stachys pilifera Benth (Lamiaceae) has long been used to treat infectious diseases, respiratory and rheumatoid disorders in Iranian folk medicine. Antitumor and antioxidant activity of the plant have been reported. OBJECTIVE: The study was designed to assess the hepatoprotective activity of ethanol extract of Stachys pilifera in carbon tetrachloride (CCl4)-induced hepatotoxicity in rats. MATERIALS AND METHODS: The rats were randomly divided into six equal groups (n = 7). Group I was treated with normal saline; Group II received CCl4 (1 mL/kg. i.p., twice a week) for 60 consecutive days; Groups III, IV and V were given CCl4 plus Stachys pilifera (100, 200 and 400 mg/kg/d,p.o.); Group VI received the extract (400 mg/kg/d, p.o.). Histopathological analysis and measurement of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), malondialdehyde (MDA), total protein (TP) and albumin (ALB) were performed. RESULTS: CCl4 caused a significant increase in the serum levels of AST, ALT, ALP and MDA as well as decreased ALB, and TP serum levels (p < 0.001). The extract (200 and 400 mg/kg/d) significantly normalized the CCl4-elevated levels of ALT, AST, ALP and MDA (p < 0.001). The extract (200 and 400 mg/kg/d) also increased the serum levels of TP compared to CCl4 group (p< 0.01). The extract (200 and 400 mg/kg/d) also decreased the histological injuries (inflammation and fatty degeneration) by CCl4. DISCUSSION: The results revealed that the Stachys pilifera extract could provide considerable protection against CCl4 hepatotoxicity in rats that may be related to its antioxidant properties. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/28317417/Hepatoprotective_effect_of_Stachys_pilifera_ethanol_extract_in_carbon_tetrachloride_induce_hepatotoxicity_in_rats_ L2 - http://www.tandfonline.com/doi/full/10.1080/13880209.2017.1302484 DB - PRIME DP - Unbound Medicine ER -