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Down-regulation of microRNA-142-5p attenuates oxygen-glucose deprivation and reoxygenation-induced neuron injury through up-regulating Nrf2/ARE signaling pathway.
Biomed Pharmacother. 2017 May; 89:1187-1195.BP

Abstract

MicroRNAs (miRNAs) play vital roles in regulating neuron survival during cerebral ischemia/reperfusion injury. miR-142-5p is reported to be an important regulator of cellular survival. However, little is known about the role of miR-142-5p in regulating neuron survival during cerebral ischemia/reperfusion injury. In this study, we aimed to investigate the precise function and mechanism of miR-142-5p in the regulation of neuron ischemia/reperfusion injury using a cellular model of oxygen-glucose deprivation and reoxygenation (OGD/R)-induced injury in hippocampal neurons in vitro. We found that miR-142-5p was induced in hippocampal neurons with OGD/R treatment. The inhibition of miR-142-5p attenuated OGD/R-induced cell injury and oxidative stress, whereas the overexpression of miR-142-5p aggravated them. Nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a target gene of miR-142-5p. Moreover, miR-142-5p regulated Nrf2 expression and downstream signaling. Knockdown of Nrf2 abolished the protective effects of miR-142-5p suppression. In addition, we showed an inverse correlation relationship between miR-142-5p and Nrf2 in an in vivo model of middle cerebral artery occlusion in rats. Taken together, these results suggest that miR-142-5p contributes to OGD/R-induced cell injury and the down-regulation of miR-142-5p attenuates OGD/R-induced neuron injury through promoting Nrf2 expression. Our study provides a novel insight into understanding the molecular pathogenesis of cerebral ischemia/reperfusion injury and indicates a potential therapeutic target for the treatment of cerebral ischemia/reperfusion injury.

Authors+Show Affiliations

Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.Department of Anesthesiology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.Department of Gastroenterology, The Second Affiliated Hospital of The Fourth Military Medical University, Xi'an 710038, China.Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China.Department of Anesthesiology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. Electronic address: lvjianruiljr@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28320085

Citation

Wang, Ning, et al. "Down-regulation of microRNA-142-5p Attenuates Oxygen-glucose Deprivation and Reoxygenation-induced Neuron Injury Through Up-regulating Nrf2/ARE Signaling Pathway." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 89, 2017, pp. 1187-1195.
Wang N, Zhang L, Lu Y, et al. Down-regulation of microRNA-142-5p attenuates oxygen-glucose deprivation and reoxygenation-induced neuron injury through up-regulating Nrf2/ARE signaling pathway. Biomed Pharmacother. 2017;89:1187-1195.
Wang, N., Zhang, L., Lu, Y., Zhang, M., Zhang, Z., Wang, K., & Lv, J. (2017). Down-regulation of microRNA-142-5p attenuates oxygen-glucose deprivation and reoxygenation-induced neuron injury through up-regulating Nrf2/ARE signaling pathway. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 89, 1187-1195. https://doi.org/10.1016/j.biopha.2017.03.011
Wang N, et al. Down-regulation of microRNA-142-5p Attenuates Oxygen-glucose Deprivation and Reoxygenation-induced Neuron Injury Through Up-regulating Nrf2/ARE Signaling Pathway. Biomed Pharmacother. 2017;89:1187-1195. PubMed PMID: 28320085.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Down-regulation of microRNA-142-5p attenuates oxygen-glucose deprivation and reoxygenation-induced neuron injury through up-regulating Nrf2/ARE signaling pathway. AU - Wang,Ning, AU - Zhang,Lingmin, AU - Lu,Yang, AU - Zhang,Mingxin, AU - Zhang,Zhenni, AU - Wang,Kui, AU - Lv,Jianrui, Y1 - 2017/03/14/ PY - 2017/01/06/received PY - 2017/02/26/revised PY - 2017/03/05/accepted PY - 2017/3/23/pubmed PY - 2018/2/15/medline PY - 2017/3/22/entrez KW - ARE KW - Cerebral ischemic/reperfusion injury KW - Nrf2 KW - miR-142-5p SP - 1187 EP - 1195 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 89 N2 - MicroRNAs (miRNAs) play vital roles in regulating neuron survival during cerebral ischemia/reperfusion injury. miR-142-5p is reported to be an important regulator of cellular survival. However, little is known about the role of miR-142-5p in regulating neuron survival during cerebral ischemia/reperfusion injury. In this study, we aimed to investigate the precise function and mechanism of miR-142-5p in the regulation of neuron ischemia/reperfusion injury using a cellular model of oxygen-glucose deprivation and reoxygenation (OGD/R)-induced injury in hippocampal neurons in vitro. We found that miR-142-5p was induced in hippocampal neurons with OGD/R treatment. The inhibition of miR-142-5p attenuated OGD/R-induced cell injury and oxidative stress, whereas the overexpression of miR-142-5p aggravated them. Nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a target gene of miR-142-5p. Moreover, miR-142-5p regulated Nrf2 expression and downstream signaling. Knockdown of Nrf2 abolished the protective effects of miR-142-5p suppression. In addition, we showed an inverse correlation relationship between miR-142-5p and Nrf2 in an in vivo model of middle cerebral artery occlusion in rats. Taken together, these results suggest that miR-142-5p contributes to OGD/R-induced cell injury and the down-regulation of miR-142-5p attenuates OGD/R-induced neuron injury through promoting Nrf2 expression. Our study provides a novel insight into understanding the molecular pathogenesis of cerebral ischemia/reperfusion injury and indicates a potential therapeutic target for the treatment of cerebral ischemia/reperfusion injury. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/28320085/Down_regulation_of_microRNA_142_5p_attenuates_oxygen_glucose_deprivation_and_reoxygenation_induced_neuron_injury_through_up_regulating_Nrf2/ARE_signaling_pathway_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(17)30079-3 DB - PRIME DP - Unbound Medicine ER -