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Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats.
Eur J Nutr. 2018 Jun; 57(4):1459-1469.EJ

Abstract

PURPOSE

Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[57Co]Cbl and CN[57Co]Cbl in Cbl-deficient and normal rats.

METHODS

Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[57Co]Cbl (free or bound to bovine transcobalamin) and free CN[57Co]Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [57Co]Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods.

RESULTS

Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p < 0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k in/k out) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma).

CONCLUSIONS

The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma.

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Authors+Show Affiliations

Kornerup LS
Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus N, Denmark. lindajen@rm.dk.
Fedosov SN
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Juul CB
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Greibe E
Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus N, Denmark.
Heegaard CW
Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
Nexo E
Department of Clinical Biochemistry, Aarhus University Hospital, Palle Juul-Jensens Boulevard 99, 8200, Aarhus N, Denmark.

MeSH

AnimalsCattleMaleRatsRats, WistarTissue DistributionVitamin B 12

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28321545

Citation

Kornerup, Linda S., et al. "Tissue Distribution of Oral Vitamin B12 Is Influenced By B12 Status and B12 Form: an Experimental Study in Rats." European Journal of Nutrition, vol. 57, no. 4, 2018, pp. 1459-1469.
Kornerup LS, Fedosov SN, Juul CB, et al. Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats. Eur J Nutr. 2018;57(4):1459-1469.
Kornerup, L. S., Fedosov, S. N., Juul, C. B., Greibe, E., Heegaard, C. W., & Nexo, E. (2018). Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats. European Journal of Nutrition, 57(4), 1459-1469. https://doi.org/10.1007/s00394-017-1424-0
Kornerup LS, et al. Tissue Distribution of Oral Vitamin B12 Is Influenced By B12 Status and B12 Form: an Experimental Study in Rats. Eur J Nutr. 2018;57(4):1459-1469. PubMed PMID: 28321545.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tissue distribution of oral vitamin B12 is influenced by B12 status and B12 form: an experimental study in rats. AU - Kornerup,Linda S, AU - Fedosov,Sergey N, AU - Juul,Christian B, AU - Greibe,Eva, AU - Heegaard,Christian W, AU - Nexo,Ebba, Y1 - 2017/03/20/ PY - 2016/09/27/received PY - 2017/02/27/accepted PY - 2017/3/23/pubmed PY - 2018/12/14/medline PY - 2017/3/22/entrez KW - Cobalamin deficiency KW - Cyanocobalamin KW - Hydroxocobalamin KW - Intestinal absorption KW - Kinetic modelling SP - 1459 EP - 1469 JF - European journal of nutrition JO - Eur J Nutr VL - 57 IS - 4 N2 - PURPOSE: Hydroxocobalamin (HOCbl) is the dominating Cbl form in food, whereas cyanocobalamin (CNCbl) is common in vitamin pills and oral supplements. This study compares single-dose absorption and distribution of oral HO[57Co]Cbl and CN[57Co]Cbl in Cbl-deficient and normal rats. METHODS: Male Wistar rats (7 weeks) were fed a 14-day diet with (n = 15) or without (n = 15) Cbl. We compared the uptakes of HO[57Co]Cbl (free or bound to bovine transcobalamin) and free CN[57Co]Cbl administered by gastric gavage (n = 5 in each diet group). Rats were sacrificed after 24 h. Blood, liver, kidney, brain, heart, spleen, intestines, skeletal muscle, 24-h urine and faeces were collected, and the content of [57Co]Cbl was measured. Endogenous Cbl in tissues and plasma was analysed by routine methods. RESULTS: Mean endogenous plasma-Cbl was sevenfold lower in deficient vs. normal rats (190 vs. 1330 pmol/L, p < 0.0001). Cbl depletion increased endogenous Cbl ratios (tissue/plasma = k in/k out) in all organs except for the kidney, where the ratio decreased considerably. Twenty-four-hour accumulation of labelled Cbl showed that HOCbl > CNCbl (liver) and CNCbl > HOCbl (brain, muscle and plasma). CONCLUSIONS: The Cbl status of rats and the administered Cbl form influence 24-h Cbl accumulation in tissues and plasma. SN - 1436-6215 UR - https://www.unboundmedicine.com/medline/citation/28321545/Tissue_distribution_of_oral_vitamin_B12_is_influenced_by_B12_status_and_B12_form:_an_experimental_study_in_rats_ L2 - https://dx.doi.org/10.1007/s00394-017-1424-0 DB - PRIME DP - Unbound Medicine ER -