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Centaurium erythraea methanol extract protects red blood cells from oxidative damage in streptozotocin-induced diabetic rats.
J Ethnopharmacol. 2017 Apr 18; 202:172-183.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Centaurium erythraea Rafn (CE) is a traditional medicinal herb in Serbia with antidiabetic, digestive, antipyretic and antiflatulent effects AIM OF THE STUDY: To investigate the potential protective effects of the methanol extract of the aerial parts of CE against glyco-oxidative stress in red blood cells (RBCs) in rats with experimentally induced diabetes.

MATERIAL AND METHODS

Diabetes was induced in Wistar rats by intraperitoneal (i.p.) injection of multiple low-dose streptozotocin (STZ) (40mg/kg, for five consecutive days), with the 1st day after the last STZ injection taken as the day of diabetes onset. The methanol extract of CE (100mg/kg) was administered orally and daily, two weeks before the first STZ injection, during the 5-day treatment with STZ, and for four weeks after the STZ injections (pre-treated group) or for four weeks after diabetes onset (post-treated group). The effect of CE extract administration on the redox status of RBCs was evaluated by assessing lipid peroxidation, the ratio of reduced/oxidized glutathione (GSH/GSSG), the level of S-glutathionylated proteins (GSSP) and the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) in RBCs four weeks after diabetes onset. The major biochemical parameters of diabetes, protein glycation/glycosylation of erythrocytes and parameters which correlate with their aggregation and deformability were also evaluated.

RESULTS

Daily application of CE extract to STZ-induced diabetic rats provided important antidiabetic effects, observed in both pre-treated and post-treated groups of diabetic rats as elevated serum insulin concentration, reduction of blood glucose and glycated hemoglobin concentrations and an improved lipid profile. Antioxidant effects of CE extract were detected in RBCs of diabetic rats and observed as decreased lipid peroxidation and ameliorated oxidative damage as a result of increased SOD, CAT and GR activities, an improved GSH/GSSG ratio and reduced GSSP levels. Moreover, the CE extract protected RBC proteins from hyperglycemia-induced damage by reducing non-enzymatic glycation and enzymatic glycosylation processes. CE extract was more effective when applied before diabetes induction (pre-treated group).

CONCLUSIONS

The results of this study show that the Centaurium erythraea methanol extract protects RBCs in diabetic animals from oxidative damage. They provide additional support for the application of this traditionally used plant in diabetes management.

Authors+Show Affiliations

Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: milos.djordjevic@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: mista@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: jelena.arambasic@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: nevenag@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: auskokovic@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: anja.tolic@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: marija.sinadinovic@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: jovana.rajic@ibiss.bg.ac.rs.Department of Plant Physiology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: dmisic@ibiss.bg.ac.rs.Department of Plant Physiology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: branislav.siler@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: goranpoz@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: melita@ibiss.bg.ac.rs.Department of Molecular Biology, Institute for Biological Research, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia. Electronic address: sdinic@ibiss.bg.ac.rs.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28323046

Citation

Đorđević, Miloš, et al. "Centaurium Erythraea Methanol Extract Protects Red Blood Cells From Oxidative Damage in Streptozotocin-induced Diabetic Rats." Journal of Ethnopharmacology, vol. 202, 2017, pp. 172-183.
Đorđević M, Mihailović M, Arambašić Jovanović J, et al. Centaurium erythraea methanol extract protects red blood cells from oxidative damage in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2017;202:172-183.
Đorđević, M., Mihailović, M., Arambašić Jovanović, J., Grdović, N., Uskoković, A., Tolić, A., Sinadinović, M., Rajić, J., Mišić, D., Šiler, B., Poznanović, G., Vidaković, M., & Dinić, S. (2017). Centaurium erythraea methanol extract protects red blood cells from oxidative damage in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 202, 172-183. https://doi.org/10.1016/j.jep.2017.03.016
Đorđević M, et al. Centaurium Erythraea Methanol Extract Protects Red Blood Cells From Oxidative Damage in Streptozotocin-induced Diabetic Rats. J Ethnopharmacol. 2017 Apr 18;202:172-183. PubMed PMID: 28323046.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Centaurium erythraea methanol extract protects red blood cells from oxidative damage in streptozotocin-induced diabetic rats. AU - Đorđević,Miloš, AU - Mihailović,Mirjana, AU - Arambašić Jovanović,Jelena, AU - Grdović,Nevena, AU - Uskoković,Aleksandra, AU - Tolić,Anja, AU - Sinadinović,Marija, AU - Rajić,Jovana, AU - Mišić,Danijela, AU - Šiler,Branislav, AU - Poznanović,Goran, AU - Vidaković,Melita, AU - Dinić,Svetlana, Y1 - 2017/03/16/ PY - 2016/12/28/received PY - 2017/02/21/revised PY - 2017/03/12/accepted PY - 2017/3/23/pubmed PY - 2017/8/25/medline PY - 2017/3/22/entrez KW - Antioxidant activity KW - Centaurium erythraea KW - Diabetes mellitus KW - Hyperglycemia KW - Oxidative stress KW - Red blood cells SP - 172 EP - 183 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 202 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Centaurium erythraea Rafn (CE) is a traditional medicinal herb in Serbia with antidiabetic, digestive, antipyretic and antiflatulent effects AIM OF THE STUDY: To investigate the potential protective effects of the methanol extract of the aerial parts of CE against glyco-oxidative stress in red blood cells (RBCs) in rats with experimentally induced diabetes. MATERIAL AND METHODS: Diabetes was induced in Wistar rats by intraperitoneal (i.p.) injection of multiple low-dose streptozotocin (STZ) (40mg/kg, for five consecutive days), with the 1st day after the last STZ injection taken as the day of diabetes onset. The methanol extract of CE (100mg/kg) was administered orally and daily, two weeks before the first STZ injection, during the 5-day treatment with STZ, and for four weeks after the STZ injections (pre-treated group) or for four weeks after diabetes onset (post-treated group). The effect of CE extract administration on the redox status of RBCs was evaluated by assessing lipid peroxidation, the ratio of reduced/oxidized glutathione (GSH/GSSG), the level of S-glutathionylated proteins (GSSP) and the enzymatic activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) in RBCs four weeks after diabetes onset. The major biochemical parameters of diabetes, protein glycation/glycosylation of erythrocytes and parameters which correlate with their aggregation and deformability were also evaluated. RESULTS: Daily application of CE extract to STZ-induced diabetic rats provided important antidiabetic effects, observed in both pre-treated and post-treated groups of diabetic rats as elevated serum insulin concentration, reduction of blood glucose and glycated hemoglobin concentrations and an improved lipid profile. Antioxidant effects of CE extract were detected in RBCs of diabetic rats and observed as decreased lipid peroxidation and ameliorated oxidative damage as a result of increased SOD, CAT and GR activities, an improved GSH/GSSG ratio and reduced GSSP levels. Moreover, the CE extract protected RBC proteins from hyperglycemia-induced damage by reducing non-enzymatic glycation and enzymatic glycosylation processes. CE extract was more effective when applied before diabetes induction (pre-treated group). CONCLUSIONS: The results of this study show that the Centaurium erythraea methanol extract protects RBCs in diabetic animals from oxidative damage. They provide additional support for the application of this traditionally used plant in diabetes management. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/28323046/Centaurium_erythraea_methanol_extract_protects_red_blood_cells_from_oxidative_damage_in_streptozotocin_induced_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)32564-8 DB - PRIME DP - Unbound Medicine ER -