Tags

Type your tag names separated by a space and hit enter

Protective effect of Nigella sativa seeds against spermatocyte chromosomal aberrations and genotoxicity induced by carbon tetrachloride in mice.
Environ Sci Pollut Res Int. 2017 Apr; 24(12):11677-11682.ES

Abstract

Nigella sativa is a well-known dietary antioxidant and a valuable inhibitor of clastogenesis and carcinogenesis. The purpose of the present work was to investigate the effects of N. sativa seeds against chromosomal aberrations in primary spermatocytes and early embryonic lethality induced by CCl4 hepatotoxin in Swiss albino mice. One hundred male Swiss albino mice were randomly divided into five groups. Groups I, II, and III received only normal saline, olive oil, and aqueous suspension of N. sativa seeds (50 mg/kg b.w.), while groups IV and V were orally given CCl4 dissolved in olive oil at a dose level of 1.9 (¼ LD50) alone and with aqueous suspension of N. sativa seeds (50 mg/kg b.w.) alternately. Aqueous extract of N. sativa significantly reduced the elevated frequency of chromosomal aberrations induced by CCl4 in mouse primary spermatocytes. For the male-dominant lethal test, four males from each group (control and experimental) were used and each male was mated for 13 days to two untreated virgin females. On days 14-16 after breeding, all the females were evaluated for incidence of pregnancy, live implants, and fetal deaths. Treatment with 1/4 LD50 of CCl4 induced positive dominant lethal mutation, reflecting a high rate of deformations in male germ cells. Interestingly, no dominant lethal mutations were recorded in females mated to male mice treated with CCl4 plus N. sativa. Under the experimental conditions of this study, our results highlight the beneficial role of N. sativa against CCl4-induced mutagenicity.

Authors+Show Affiliations

Department of Biological Sciences, Faculty of Science, King Faisal University, Al-Ahsa, Saudi Arabia. aabdelmoneim@kfu.edu.sa. Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt. aabdelmoneim@kfu.edu.sa.Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt. Department of Biology, Faculty of Science, King Saud University, Riyadh, Saudi Arabia.Genetics Branch, Department of Botany Faculty of Agriculture, Saba Basha, Alexandria University, Alexandria, Egypt.Department of Zoology, Faculty of Science, Alexandria University, Alexandria, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28324259

Citation

Abdel-Moneim, Ashraf M., et al. "Protective Effect of Nigella Sativa Seeds Against Spermatocyte Chromosomal Aberrations and Genotoxicity Induced By Carbon Tetrachloride in Mice." Environmental Science and Pollution Research International, vol. 24, no. 12, 2017, pp. 11677-11682.
Abdel-Moneim AM, Essawy AE, Hamed SS, et al. Protective effect of Nigella sativa seeds against spermatocyte chromosomal aberrations and genotoxicity induced by carbon tetrachloride in mice. Environ Sci Pollut Res Int. 2017;24(12):11677-11682.
Abdel-Moneim, A. M., Essawy, A. E., Hamed, S. S., Abou-Gabal, A. A., & Alzergy, A. A. (2017). Protective effect of Nigella sativa seeds against spermatocyte chromosomal aberrations and genotoxicity induced by carbon tetrachloride in mice. Environmental Science and Pollution Research International, 24(12), 11677-11682. https://doi.org/10.1007/s11356-017-8806-y
Abdel-Moneim AM, et al. Protective Effect of Nigella Sativa Seeds Against Spermatocyte Chromosomal Aberrations and Genotoxicity Induced By Carbon Tetrachloride in Mice. Environ Sci Pollut Res Int. 2017;24(12):11677-11682. PubMed PMID: 28324259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effect of Nigella sativa seeds against spermatocyte chromosomal aberrations and genotoxicity induced by carbon tetrachloride in mice. AU - Abdel-Moneim,Ashraf M, AU - Essawy,Amina E, AU - Hamed,Sherifa S, AU - Abou-Gabal,Ashgan A, AU - Alzergy,Aglal A, Y1 - 2017/03/21/ PY - 2016/12/13/received PY - 2017/03/10/accepted PY - 2017/3/23/pubmed PY - 2017/6/20/medline PY - 2017/3/22/entrez KW - Carbon tetrachloride KW - Chromosomal aberrations KW - Dominant lethal mutation KW - Genotoxicity KW - Germ cells KW - Nigella sativa SP - 11677 EP - 11682 JF - Environmental science and pollution research international JO - Environ Sci Pollut Res Int VL - 24 IS - 12 N2 - Nigella sativa is a well-known dietary antioxidant and a valuable inhibitor of clastogenesis and carcinogenesis. The purpose of the present work was to investigate the effects of N. sativa seeds against chromosomal aberrations in primary spermatocytes and early embryonic lethality induced by CCl4 hepatotoxin in Swiss albino mice. One hundred male Swiss albino mice were randomly divided into five groups. Groups I, II, and III received only normal saline, olive oil, and aqueous suspension of N. sativa seeds (50 mg/kg b.w.), while groups IV and V were orally given CCl4 dissolved in olive oil at a dose level of 1.9 (¼ LD50) alone and with aqueous suspension of N. sativa seeds (50 mg/kg b.w.) alternately. Aqueous extract of N. sativa significantly reduced the elevated frequency of chromosomal aberrations induced by CCl4 in mouse primary spermatocytes. For the male-dominant lethal test, four males from each group (control and experimental) were used and each male was mated for 13 days to two untreated virgin females. On days 14-16 after breeding, all the females were evaluated for incidence of pregnancy, live implants, and fetal deaths. Treatment with 1/4 LD50 of CCl4 induced positive dominant lethal mutation, reflecting a high rate of deformations in male germ cells. Interestingly, no dominant lethal mutations were recorded in females mated to male mice treated with CCl4 plus N. sativa. Under the experimental conditions of this study, our results highlight the beneficial role of N. sativa against CCl4-induced mutagenicity. SN - 1614-7499 UR - https://www.unboundmedicine.com/medline/citation/28324259/Protective_effect_of_Nigella_sativa_seeds_against_spermatocyte_chromosomal_aberrations_and_genotoxicity_induced_by_carbon_tetrachloride_in_mice_ L2 - https://dx.doi.org/10.1007/s11356-017-8806-y DB - PRIME DP - Unbound Medicine ER -