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Cellulase-assisted extraction of polysaccharides from Malva sylvestris: Process optimization and potential functionalities.
Int J Biol Macromol. 2017 Aug; 101:196-206.IJ

Abstract

Enzyme-assisted extraction process of the water-soluble Malva sylvestris polysaccharides (MSPs) was optimized using response surface methodology (RSM). The highest yield (10.40%) of MSPs was achieved at 5.64% cellulase, 55.65°C temperature, 3.4h time, and 5.22 pH. Three homogeneous polysaccharide fractions (MSP-1, MSP-2, MSP-3) were purified by DEAE-cellulose and Sephadex G-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar ratios with molecular weight range of 2.6×105-8.8×105Da. The fractions could significantly increase antioxidant, antitumor and antimicrobial activities in a dose-dependent pattern. MSP-2 revealed stronger antioxidant activities than MSP-1 and MSP-3, including reducing power and scavenging activity of DPPH and OH radicals. The antiproliferative activity of MSP-2 (1.0mg/mL) on the growth of A549 and HepG2 cells was 45.1% and 53.2%, respectively. The Gram-positive bacteria (Bacillus cereus PTCC 1015 and Staphylococcus aureus PTCC 1112) compared with Gram-negative ones (Escherichia coli PTCC 1763 and Salmonella typhimurium PTCC 1709) showed less sensitivity against the various MSPs (3-15mg/mL).

Authors+Show Affiliations

Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran 14778-93855, Iran. Electronic address: smt.gharibzahedi@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28327424

Citation

Rostami, Hosein, and Seyed Mohammad Taghi Gharibzahedi. "Cellulase-assisted Extraction of Polysaccharides From Malva Sylvestris: Process Optimization and Potential Functionalities." International Journal of Biological Macromolecules, vol. 101, 2017, pp. 196-206.
Rostami H, Gharibzahedi SMT. Cellulase-assisted extraction of polysaccharides from Malva sylvestris: Process optimization and potential functionalities. Int J Biol Macromol. 2017;101:196-206.
Rostami, H., & Gharibzahedi, S. M. T. (2017). Cellulase-assisted extraction of polysaccharides from Malva sylvestris: Process optimization and potential functionalities. International Journal of Biological Macromolecules, 101, 196-206. https://doi.org/10.1016/j.ijbiomac.2017.03.078
Rostami H, Gharibzahedi SMT. Cellulase-assisted Extraction of Polysaccharides From Malva Sylvestris: Process Optimization and Potential Functionalities. Int J Biol Macromol. 2017;101:196-206. PubMed PMID: 28327424.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cellulase-assisted extraction of polysaccharides from Malva sylvestris: Process optimization and potential functionalities. AU - Rostami,Hosein, AU - Gharibzahedi,Seyed Mohammad Taghi, Y1 - 2017/03/19/ PY - 2016/09/15/received PY - 2017/03/02/revised PY - 2017/03/14/accepted PY - 2017/3/23/pubmed PY - 2018/1/26/medline PY - 2017/3/23/entrez KW - Antibacterial KW - Antioxidant KW - Antitumor KW - Common mallow polysaccharide KW - Enzyme assisted extraction KW - Optimization SP - 196 EP - 206 JF - International journal of biological macromolecules JO - Int J Biol Macromol VL - 101 N2 - Enzyme-assisted extraction process of the water-soluble Malva sylvestris polysaccharides (MSPs) was optimized using response surface methodology (RSM). The highest yield (10.40%) of MSPs was achieved at 5.64% cellulase, 55.65°C temperature, 3.4h time, and 5.22 pH. Three homogeneous polysaccharide fractions (MSP-1, MSP-2, MSP-3) were purified by DEAE-cellulose and Sephadex G-100 chromatography, which were composed of galactose, glucuronic acid, arabinose, rhamnose and mannose in different molar ratios with molecular weight range of 2.6×105-8.8×105Da. The fractions could significantly increase antioxidant, antitumor and antimicrobial activities in a dose-dependent pattern. MSP-2 revealed stronger antioxidant activities than MSP-1 and MSP-3, including reducing power and scavenging activity of DPPH and OH radicals. The antiproliferative activity of MSP-2 (1.0mg/mL) on the growth of A549 and HepG2 cells was 45.1% and 53.2%, respectively. The Gram-positive bacteria (Bacillus cereus PTCC 1015 and Staphylococcus aureus PTCC 1112) compared with Gram-negative ones (Escherichia coli PTCC 1763 and Salmonella typhimurium PTCC 1709) showed less sensitivity against the various MSPs (3-15mg/mL). SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/28327424/Cellulase_assisted_extraction_of_polysaccharides_from_Malva_sylvestris:_Process_optimization_and_potential_functionalities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(16)31651-8 DB - PRIME DP - Unbound Medicine ER -