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Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways.
Cancer Med 2017; 6(4):819-833CM

Abstract

We aimed to detect the effects of miR-145-5p on the cell proliferation, apoptosis, migration, and invasion in NRAS-mutant, BRAF-mutant, and wild-type melanoma cells, in order to figure out the potential mechanisms and provide a novel therapeutic target of melanoma. RT-qPCR and western blot were used to detect the expression of miR-145-5p and NRAS in melanoma tumor tissues and cells, respectively. Luciferase assay was performed to determine whether miR-145-5p directly targeted NRAS. After transfecting miR-145-5p mimics, miR-145-5p inhibitors, NRAS cDNA and NRAS siRNA into CHL-1, VMM917 and SK-mel-28 cells, functional assays were used to detect the proliferation, apoptosis, invasion and migration, including MTT, flow cytometry, Transwell and wound healing assays. In addition, xenograft models in nude mice were also conducted to verify the role of miR-145-5p in vivo. MiR-145-5p was able to suppress proliferation, invasion, and migration of VMM917 and CHL-1 cells and induce apoptosis by inhibiting MAPK and PI3K/AKT pathways. However, aberrant expression of miR-145-5p and NRAS has little impact on the viability and metastasis of BRAF-mutant melanoma. The higher expression of miR-145-5p in xenograft models repressed the VMM917-induced and CHL-1-induced tumor growth observably and has little effect on SK-mel-28-induced tumor growth which was consistent with the results in vitro. Through targeting NRAS, miR-145-5p could suppress cell proliferation, invasion, and migration and induce apoptosis of CHL-1 and VMM917 melanoma cells by inhibiting MAPK and PI3K/AKT pathways.

Authors+Show Affiliations

Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China. Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia, 010051, China.Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia, 010051, China.Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia, 010051, China.Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia, 010051, China.Department of Burn and Plastic Surgery, The 253rd Hospital of PLA, Hohhot, Inner Mongolia, 010051, China.Department of Plastic and Reconstructive Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.Department of Otolaryngology-Head and Neck Surgery, The Fourth People's Hospital of Shaanxi Province, Xi'an, 710043, China.Department of Otolaryngology-Head and Neck Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, 710032, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28332309

Citation

Liu, Sha, et al. "Effects of miR-145-5p Through NRAS On the Cell Proliferation, Apoptosis, Migration, and Invasion in Melanoma By Inhibiting MAPK and PI3K/AKT Pathways." Cancer Medicine, vol. 6, no. 4, 2017, pp. 819-833.
Liu S, Gao G, Yan D, et al. Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways. Cancer Med. 2017;6(4):819-833.
Liu, S., Gao, G., Yan, D., Chen, X., Yao, X., Guo, S., ... Zhao, Y. (2017). Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways. Cancer Medicine, 6(4), pp. 819-833. doi:10.1002/cam4.1030.
Liu S, et al. Effects of miR-145-5p Through NRAS On the Cell Proliferation, Apoptosis, Migration, and Invasion in Melanoma By Inhibiting MAPK and PI3K/AKT Pathways. Cancer Med. 2017;6(4):819-833. PubMed PMID: 28332309.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of miR-145-5p through NRAS on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting MAPK and PI3K/AKT pathways. AU - Liu,Sha, AU - Gao,Guozhen, AU - Yan,Dexiong, AU - Chen,Xiangjun, AU - Yao,Xingwei, AU - Guo,Shuzhong, AU - Li,Guirong, AU - Zhao,Yu, Y1 - 2017/03/23/ PY - 2016/09/19/received PY - 2017/01/09/revised PY - 2017/01/16/accepted PY - 2017/3/24/pubmed PY - 2017/11/11/medline PY - 2017/3/24/entrez KW - MAPK KW - NRAS KW - melanoma KW - miR-145-5p SP - 819 EP - 833 JF - Cancer medicine JO - Cancer Med VL - 6 IS - 4 N2 - We aimed to detect the effects of miR-145-5p on the cell proliferation, apoptosis, migration, and invasion in NRAS-mutant, BRAF-mutant, and wild-type melanoma cells, in order to figure out the potential mechanisms and provide a novel therapeutic target of melanoma. RT-qPCR and western blot were used to detect the expression of miR-145-5p and NRAS in melanoma tumor tissues and cells, respectively. Luciferase assay was performed to determine whether miR-145-5p directly targeted NRAS. After transfecting miR-145-5p mimics, miR-145-5p inhibitors, NRAS cDNA and NRAS siRNA into CHL-1, VMM917 and SK-mel-28 cells, functional assays were used to detect the proliferation, apoptosis, invasion and migration, including MTT, flow cytometry, Transwell and wound healing assays. In addition, xenograft models in nude mice were also conducted to verify the role of miR-145-5p in vivo. MiR-145-5p was able to suppress proliferation, invasion, and migration of VMM917 and CHL-1 cells and induce apoptosis by inhibiting MAPK and PI3K/AKT pathways. However, aberrant expression of miR-145-5p and NRAS has little impact on the viability and metastasis of BRAF-mutant melanoma. The higher expression of miR-145-5p in xenograft models repressed the VMM917-induced and CHL-1-induced tumor growth observably and has little effect on SK-mel-28-induced tumor growth which was consistent with the results in vitro. Through targeting NRAS, miR-145-5p could suppress cell proliferation, invasion, and migration and induce apoptosis of CHL-1 and VMM917 melanoma cells by inhibiting MAPK and PI3K/AKT pathways. SN - 2045-7634 UR - https://www.unboundmedicine.com/medline/citation/28332309/Effects_of_miR_145_5p_through_NRAS_on_the_cell_proliferation_apoptosis_migration_and_invasion_in_melanoma_by_inhibiting_MAPK_and_PI3K/AKT_pathways_ L2 - https://doi.org/10.1002/cam4.1030 DB - PRIME DP - Unbound Medicine ER -