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Cross-sectional surveillance of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels and other mammals in Egypt, August 2015 to January 2016.
Euro Surveill. 2017 Mar 16; 22(11)ES

Abstract

A cross-sectional study was conducted in Egypt to determine the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) in imported and resident camels and bats, as well as to assess possible transmission of the virus to domestic ruminants and equines. A total of 1,031 sera, 1,078 nasal swabs, 13 rectal swabs, and 38 milk samples were collected from 1,078 camels in different types of sites. In addition, 145 domestic animals and 109 bats were sampled. Overall, of 1,031 serologically-tested camels, 871 (84.5%) had MERS-CoV neutralising antibodies. Seroprevalence was significantly higher in imported (614/692; 88.7%) than resident camels (257/339; 5.8%) (p < 0.05). Camels from Sudan (543/594; 91.4%) had a higher seroprevalence than those from East Africa (71/98; 72.4%) (p < 0.05). Sampling site and age were also associated with MERS-CoV seroprevalence (p < 0.05). All tested samples from domestic animals and bats were negative for MERS-CoV antibodies except one sheep sample which showed a 1:640 titre. Of 1,078 camels, 41 (3.8%) were positive for MERS-CoV genetic material. Sequences obtained were not found to cluster with clade A or B MERS-CoV sequences and were genetically diverse. The presence of neutralising antibodies in one sheep apparently in contact with seropositive camels calls for further studies on domestic animals in contact with camels.

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Authors+Show Affiliations

Ali M
National Research Center, Division of Environmental Research, Giza, Egypt.
El-Shesheny R
National Research Center, Division of Environmental Research, Giza, Egypt.
Kandeil A
National Research Center, Division of Environmental Research, Giza, Egypt.
Shehata M
National Research Center, Division of Environmental Research, Giza, Egypt.
Elsokary B
General Organizations of Veterinary Services, Ministry of Agriculture and Land reclamation (MoALR), Giza, Egypt.
Gomaa M
National Research Center, Division of Environmental Research, Giza, Egypt.
Hassan N
General Organizations of Veterinary Services, Ministry of Agriculture and Land reclamation (MoALR), Giza, Egypt.
El Sayed A
National Research Center, Division of Environmental Research, Giza, Egypt.
El-Taweel A
National Research Center, Division of Environmental Research, Giza, Egypt.
Sobhy H
Food and Agriculture Organization of the United Nations, Emergency Center for Transboundary Animal Diseases (ECTAD), Egypt.
Fasina FO
Food and Agriculture Organization of the United Nations, Emergency Center for Transboundary Animal Diseases (ECTAD), Egypt. Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, South Africa.
Dauphin G
Food and Agriculture Organization of the United Nations, Rome, Italy.
El Masry I
Food and Agriculture Organization of the United Nations, Emergency Center for Transboundary Animal Diseases (ECTAD), Egypt.
Wolde AW
Food and Agriculture Organization of the United Nations, Emergency Center for Transboundary Animal Diseases (ECTAD), Egypt.
Daszak P
EcoHealth Alliance, New York, New York, United States.
Miller M
EcoHealth Alliance, New York, New York, United States.
VonDobschuetz S
Food and Agriculture Organization of the United Nations, Rome, Italy.
Morzaria S
Food and Agriculture Organization of the United Nations, Rome, Italy.
Lubroth J
Food and Agriculture Organization of the United Nations, Rome, Italy.
Makonnen YJ
Food and Agriculture Organization of the United Nations, Emergency Center for Transboundary Animal Diseases (ECTAD), Egypt.

MeSH

AnimalsAntibodies, NeutralizingAntibodies, ViralBase SequenceCamelusCattleCoronavirusCoronavirus InfectionsCross-Sectional StudiesDisease OutbreaksDisease ReservoirsEgyptGenotypeGoatsImmunoglobulin GMiddle East Respiratory Syndrome CoronavirusMolecular Sequence DataPrevalenceRNA, ViralReverse Transcriptase Polymerase Chain ReactionSequence Analysis, RNASeroepidemiologic StudiesSheepZoonoses

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28333616

Citation

Ali, Mohamed, et al. "Cross-sectional Surveillance of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels and Other Mammals in Egypt, August 2015 to January 2016." Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin, vol. 22, no. 11, 2017.
Ali M, El-Shesheny R, Kandeil A, et al. Cross-sectional surveillance of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels and other mammals in Egypt, August 2015 to January 2016. Euro Surveill. 2017;22(11).
Ali, M., El-Shesheny, R., Kandeil, A., Shehata, M., Elsokary, B., Gomaa, M., Hassan, N., El Sayed, A., El-Taweel, A., Sobhy, H., Fasina, F. O., Dauphin, G., El Masry, I., Wolde, A. W., Daszak, P., Miller, M., VonDobschuetz, S., Morzaria, S., Lubroth, J., & Makonnen, Y. J. (2017). Cross-sectional surveillance of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels and other mammals in Egypt, August 2015 to January 2016. Euro Surveillance : Bulletin Europeen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin, 22(11). https://doi.org/10.2807/1560-7917.ES.2017.22.11.30487
Ali M, et al. Cross-sectional Surveillance of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels and Other Mammals in Egypt, August 2015 to January 2016. Euro Surveill. 2017 Mar 16;22(11) PubMed PMID: 28333616.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cross-sectional surveillance of Middle East respiratory syndrome coronavirus (MERS-CoV) in dromedary camels and other mammals in Egypt, August 2015 to January 2016. AU - Ali,Mohamed, AU - El-Shesheny,Rabeh, AU - Kandeil,Ahmed, AU - Shehata,Mahmoud, AU - Elsokary,Basma, AU - Gomaa,Mokhtar, AU - Hassan,Naglaa, AU - El Sayed,Ahmed, AU - El-Taweel,Ahmed, AU - Sobhy,Heba, AU - Fasina,Folorunso Oludayo, AU - Dauphin,Gwenaelle, AU - El Masry,Ihab, AU - Wolde,Abebe Wossene, AU - Daszak,Peter, AU - Miller,Maureen, AU - VonDobschuetz,Sophie, AU - Morzaria,Subhash, AU - Lubroth,Juan, AU - Makonnen,Yilma Jobre, PY - 2016/04/10/received PY - 2016/10/05/accepted PY - 2017/3/24/entrez PY - 2017/3/24/pubmed PY - 2017/5/30/medline KW - Camel, Ruminants KW - Egypt KW - Equines KW - MERS-CoV KW - bats JF - Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin JO - Euro Surveill VL - 22 IS - 11 N2 - A cross-sectional study was conducted in Egypt to determine the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) in imported and resident camels and bats, as well as to assess possible transmission of the virus to domestic ruminants and equines. A total of 1,031 sera, 1,078 nasal swabs, 13 rectal swabs, and 38 milk samples were collected from 1,078 camels in different types of sites. In addition, 145 domestic animals and 109 bats were sampled. Overall, of 1,031 serologically-tested camels, 871 (84.5%) had MERS-CoV neutralising antibodies. Seroprevalence was significantly higher in imported (614/692; 88.7%) than resident camels (257/339; 5.8%) (p < 0.05). Camels from Sudan (543/594; 91.4%) had a higher seroprevalence than those from East Africa (71/98; 72.4%) (p < 0.05). Sampling site and age were also associated with MERS-CoV seroprevalence (p < 0.05). All tested samples from domestic animals and bats were negative for MERS-CoV antibodies except one sheep sample which showed a 1:640 titre. Of 1,078 camels, 41 (3.8%) were positive for MERS-CoV genetic material. Sequences obtained were not found to cluster with clade A or B MERS-CoV sequences and were genetically diverse. The presence of neutralising antibodies in one sheep apparently in contact with seropositive camels calls for further studies on domestic animals in contact with camels. SN - 1560-7917 UR - https://www.unboundmedicine.com/medline/citation/28333616/Cross_sectional_surveillance_of_Middle_East_respiratory_syndrome_coronavirus__MERS_CoV__in_dromedary_camels_and_other_mammals_in_Egypt_August_2015_to_January_2016_ DB - PRIME DP - Unbound Medicine ER -