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[Comparative analysis of the structure of incompatibility plasmids N, P and W].

Abstract

Evolutionary relationships of the IncN plasmid R15 and other broad host range plasmids (IncN plasmids N3 and R46, IncP plasmids RP4 and R906, IncW plasmids Sa and R388) were studied by Southern blot hybridization technique. The IncN plasmids were shown to harbour homologous determinants for replication and conjugation. No homology was found between the rep and tra genes in R15 and in the IncW and IncP plasmids, respectively. The second rep region of the N3 plasmid is distinctive from the corresponding determinants in the IncN plasmids. Homology was demonstrated for the plasmid genes that mediate restriction and modification in R15 and N3, mercury resistance in R15 and R906, sulfanilamide resistance in R15, N3, R46, Sa, R388, and R906, streptomycin resistance in R15, R46 and Sa. The latter genes are different from the R906 SmR gene. In addition to the three known mobile elements in the plasmid R15, the fourth one (IS46) that is a part of the transposon Tn2353 was identified in this study. Besides, the third copy of this insertion sequence was found in the N3 plasmid.

Authors

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Pub Type(s)

Comparative Study
English Abstract
Journal Article

Language

rus

PubMed ID

2833696

Citation

Dobritsa, A P., et al. "[Comparative Analysis of the Structure of Incompatibility Plasmids N, P and W]." Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, 1987, pp. 3-11.
Dobritsa AP, Dergunova LV, Mikhaĭlova TG, et al. [Comparative analysis of the structure of incompatibility plasmids N, P and W]. Mol Gen Mikrobiol Virusol. 1987.
Dobritsa, A. P., Dergunova, L. V., Mikhaĭlova, T. G., & Zimin, A. A. (1987). [Comparative analysis of the structure of incompatibility plasmids N, P and W]. Molekuliarnaia Genetika, Mikrobiologiia I Virusologiia, (12), 3-11.
Dobritsa AP, et al. [Comparative Analysis of the Structure of Incompatibility Plasmids N, P and W]. Mol Gen Mikrobiol Virusol. 1987;(12)3-11. PubMed PMID: 2833696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparative analysis of the structure of incompatibility plasmids N, P and W]. AU - Dobritsa,A P, AU - Dergunova,L V, AU - Mikhaĭlova,T G, AU - Zimin,A A, PY - 1987/12/1/pubmed PY - 1987/12/1/medline PY - 1987/12/1/entrez SP - 3 EP - 11 JF - Molekuliarnaia genetika, mikrobiologiia i virusologiia JO - Mol. Gen. Mikrobiol. Virusol. IS - 12 N2 - Evolutionary relationships of the IncN plasmid R15 and other broad host range plasmids (IncN plasmids N3 and R46, IncP plasmids RP4 and R906, IncW plasmids Sa and R388) were studied by Southern blot hybridization technique. The IncN plasmids were shown to harbour homologous determinants for replication and conjugation. No homology was found between the rep and tra genes in R15 and in the IncW and IncP plasmids, respectively. The second rep region of the N3 plasmid is distinctive from the corresponding determinants in the IncN plasmids. Homology was demonstrated for the plasmid genes that mediate restriction and modification in R15 and N3, mercury resistance in R15 and R906, sulfanilamide resistance in R15, N3, R46, Sa, R388, and R906, streptomycin resistance in R15, R46 and Sa. The latter genes are different from the R906 SmR gene. In addition to the three known mobile elements in the plasmid R15, the fourth one (IS46) that is a part of the transposon Tn2353 was identified in this study. Besides, the third copy of this insertion sequence was found in the N3 plasmid. SN - 0208-0613 UR - https://www.unboundmedicine.com/medline/citation/2833696/[Comparative_analysis_of_the_structure_of_incompatibility_plasmids_N_P_and_W]_ DB - PRIME DP - Unbound Medicine ER -