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CRLF2-Positive B-Cell Acute Lymphoblastic Leukemia in Adult Patients: A Single-Institution Experience.
Am J Clin Pathol 2017; 147(4):357-363AJ

Abstract

Objectives

Cytokine receptor-like factor 2 ( CRLF2 ) rearrangement is found in approximately 50% of pediatric Ph-like B-cell acute lymphoblastic leukemia (B-ALL), and around 50% of CRLF2 + cases harbor JAK mutations. We analyzed CRLF2 expression and studied its correlation with CRLF2 rearrangement in adult patients with B-ALL.

Methods

Multiparameter flow cytometry (MFC) was performed consecutively in 126 patients.

Results

CRLF2 overexpression was detected in 30 (27%) patients, 28 (41%) of 69 patients with B-ALL not otherwise specified, 14 (21%) of 67 untreated patients, and 16 (27%) of 59 patients with relapsed B-ALL, with the highest among Hispanic patients (25/55, 45%). Of CRLF2+ cases, 21 (100%) of 21 cases showed CRLF2 rearrangement by fluorescence in situ hybridization, preferentially involving IGH@CRLF2 (15/15). The entire coding region of JAK2 was sequenced in 14 patients with CRLF2+ B-ALL, and nine (64%) were positive for JAK2 mutations.

Conclusions

MFC allows a rapid, inexpensive, and reliable detection of B-ALL with CRLF2 rearrangement that would further facilitate testing for JAK2 mutations for targetable therapy.

Authors+Show Affiliations

From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.Department of Pathology, St Jude Children's Research Hospital, Memphis, TN.Department of Pathology, St Jude Children's Research Hospital, Memphis, TN.Department of Pathology, St Jude Children's Research Hospital, Memphis, TN.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.Pediatrics, UT MD Anderson Cancer Center, Houston, TX.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.From the Departments of Hematopathology and Leukemia, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28340183

Citation

Konoplev, Sergej, et al. "CRLF2-Positive B-Cell Acute Lymphoblastic Leukemia in Adult Patients: a Single-Institution Experience." American Journal of Clinical Pathology, vol. 147, no. 4, 2017, pp. 357-363.
Konoplev S, Lu X, Konopleva M, et al. CRLF2-Positive B-Cell Acute Lymphoblastic Leukemia in Adult Patients: A Single-Institution Experience. Am J Clin Pathol. 2017;147(4):357-363.
Konoplev, S., Lu, X., Konopleva, M., Jain, N., Ouyang, J., Goswami, M., ... Wang, S. A. (2017). CRLF2-Positive B-Cell Acute Lymphoblastic Leukemia in Adult Patients: A Single-Institution Experience. American Journal of Clinical Pathology, 147(4), pp. 357-363. doi:10.1093/ajcp/aqx005.
Konoplev S, et al. CRLF2-Positive B-Cell Acute Lymphoblastic Leukemia in Adult Patients: a Single-Institution Experience. Am J Clin Pathol. 2017 04 1;147(4):357-363. PubMed PMID: 28340183.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CRLF2-Positive B-Cell Acute Lymphoblastic Leukemia in Adult Patients: A Single-Institution Experience. AU - Konoplev,Sergej, AU - Lu,Xinyan, AU - Konopleva,Marina, AU - Jain,Nitin, AU - Ouyang,Juan, AU - Goswami,Maitrayee, AU - Roberts,Kathryn G, AU - Valentine,Marc, AU - Mullighan,Charles G, AU - Bueso-Ramos,Carlos, AU - Zweidler-McKay,Patrick A, AU - Jorgensen,Jeffrey L, AU - Wang,Sa A, PY - 2017/3/25/pubmed PY - 2017/5/4/medline PY - 2017/3/25/entrez KW - B-lymphoblastic leukemia/lymphoma KW - BCR-ABL1–like KW - CRLF2 KW - FISH KW - Flow cytometry KW - JAK2 mutation KW - Ph-like B-ALL SP - 357 EP - 363 JF - American journal of clinical pathology JO - Am. J. Clin. Pathol. VL - 147 IS - 4 N2 - Objectives: Cytokine receptor-like factor 2 ( CRLF2 ) rearrangement is found in approximately 50% of pediatric Ph-like B-cell acute lymphoblastic leukemia (B-ALL), and around 50% of CRLF2 + cases harbor JAK mutations. We analyzed CRLF2 expression and studied its correlation with CRLF2 rearrangement in adult patients with B-ALL. Methods: Multiparameter flow cytometry (MFC) was performed consecutively in 126 patients. Results: CRLF2 overexpression was detected in 30 (27%) patients, 28 (41%) of 69 patients with B-ALL not otherwise specified, 14 (21%) of 67 untreated patients, and 16 (27%) of 59 patients with relapsed B-ALL, with the highest among Hispanic patients (25/55, 45%). Of CRLF2+ cases, 21 (100%) of 21 cases showed CRLF2 rearrangement by fluorescence in situ hybridization, preferentially involving IGH@CRLF2 (15/15). The entire coding region of JAK2 was sequenced in 14 patients with CRLF2+ B-ALL, and nine (64%) were positive for JAK2 mutations. Conclusions: MFC allows a rapid, inexpensive, and reliable detection of B-ALL with CRLF2 rearrangement that would further facilitate testing for JAK2 mutations for targetable therapy. SN - 1943-7722 UR - https://www.unboundmedicine.com/medline/citation/28340183/CRLF2_Positive_B_Cell_Acute_Lymphoblastic_Leukemia_in_Adult_Patients:_A_Single_Institution_Experience_ L2 - https://academic.oup.com/ajcp/article-lookup/doi/10.1093/ajcp/aqx005 DB - PRIME DP - Unbound Medicine ER -