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Breviscapine confers a neuroprotective efficacy against transient focal cerebral ischemia by attenuating neuronal and astrocytic autophagy in the penumbra.
Biomed Pharmacother. 2017 Jun; 90:69-76.BP

Abstract

Breviscapine is a flavonoid derived from a traditional Chinese herb Erigerin breviscapus (Vant.) Hand-Mazz, and has been extensively used in clinical treatment for cerebral stroke in China, but the underlying pharmacological mechanisms are still unclear. In present study, we investigated whether breviscapine could confer a neuroprotection against cerebral ischemia injury by targeting autophagy mechanisms. A cerebral stroke model in Sprague-Dawley rats was prepared by middle cerebral artery occlusion (MCAO), rats were then randomly divided into 5 groups: MCAO+Bre group, rats were treated with breviscapine; MCAO+Tat-Beclin-1 group, animals were administrated with specific autophagy inducer Tat-Beclin-1; MCAO+Bre+Tat-Beclin-1 group, rats were treated with both breviscapine and Tat-Beclin-1, MCAO+saline group, rats received the same volume of physiological saline, and Sham surgery group. The autophagy levels in infarct penumbra were evaluated by western blotting, real-time PCR and immunofluorescence 7days after the insult. Meanwhile, infarct volume, brain water content and neurological deficit score were assessed. The results illustrated that the infarct volume, brain water content and neurofunctional deficiency were significantly reduced by 7days of breviscapine treatment in MCAO+Bre group, compared with those in MCAO+saline group. Meanwhile, the western blotting, quantitative PCR and immunofluorescence showed that the autophagy in both neurons and astrocytes at the penumbra were markedly attenuated by breviscapine admininstration. Moreover, these pharmacological effects of breviscapine could be counteracted by autophagy inducer Tat-Beclin-1. Our study suggests that breviscapine can provide a neuroprotection against transient focal cerebral ischemia, and this biological function is associated with attenuating autophagy in both neurons and astrocytes.

Authors+Show Affiliations

Department of morphology, Medical School, Kunming University of Science and Technology, Kunming 650500, China.Department of morphology, Medical School, Kunming University of Science and Technology, Kunming 650500, China.Department of morphology, Medical School, Kunming University of Science and Technology, Kunming 650500, China.Department of morphology, Medical School, Kunming University of Science and Technology, Kunming 650500, China.Department of morphology, Medical School, Kunming University of Science and Technology, Kunming 650500, China. Electronic address: deng13032871868@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28343073

Citation

Pengyue, Zhang, et al. "Breviscapine Confers a Neuroprotective Efficacy Against Transient Focal Cerebral Ischemia By Attenuating Neuronal and Astrocytic Autophagy in the Penumbra." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 90, 2017, pp. 69-76.
Pengyue Z, Tao G, Hongyun H, et al. Breviscapine confers a neuroprotective efficacy against transient focal cerebral ischemia by attenuating neuronal and astrocytic autophagy in the penumbra. Biomed Pharmacother. 2017;90:69-76.
Pengyue, Z., Tao, G., Hongyun, H., Liqiang, Y., & Yihao, D. (2017). Breviscapine confers a neuroprotective efficacy against transient focal cerebral ischemia by attenuating neuronal and astrocytic autophagy in the penumbra. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 90, 69-76. https://doi.org/10.1016/j.biopha.2017.03.039
Pengyue Z, et al. Breviscapine Confers a Neuroprotective Efficacy Against Transient Focal Cerebral Ischemia By Attenuating Neuronal and Astrocytic Autophagy in the Penumbra. Biomed Pharmacother. 2017;90:69-76. PubMed PMID: 28343073.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Breviscapine confers a neuroprotective efficacy against transient focal cerebral ischemia by attenuating neuronal and astrocytic autophagy in the penumbra. AU - Pengyue,Zhang, AU - Tao,Guo, AU - Hongyun,He, AU - Liqiang,Yang, AU - Yihao,Deng, Y1 - 2017/03/24/ PY - 2016/12/16/received PY - 2017/03/06/revised PY - 2017/03/14/accepted PY - 2017/3/28/pubmed PY - 2018/2/21/medline PY - 2017/3/27/entrez KW - Autophagy inhibition KW - Breviscapine KW - Cerebral ischemia KW - Neuroprotection KW - Penumbra SP - 69 EP - 76 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed. Pharmacother. VL - 90 N2 - Breviscapine is a flavonoid derived from a traditional Chinese herb Erigerin breviscapus (Vant.) Hand-Mazz, and has been extensively used in clinical treatment for cerebral stroke in China, but the underlying pharmacological mechanisms are still unclear. In present study, we investigated whether breviscapine could confer a neuroprotection against cerebral ischemia injury by targeting autophagy mechanisms. A cerebral stroke model in Sprague-Dawley rats was prepared by middle cerebral artery occlusion (MCAO), rats were then randomly divided into 5 groups: MCAO+Bre group, rats were treated with breviscapine; MCAO+Tat-Beclin-1 group, animals were administrated with specific autophagy inducer Tat-Beclin-1; MCAO+Bre+Tat-Beclin-1 group, rats were treated with both breviscapine and Tat-Beclin-1, MCAO+saline group, rats received the same volume of physiological saline, and Sham surgery group. The autophagy levels in infarct penumbra were evaluated by western blotting, real-time PCR and immunofluorescence 7days after the insult. Meanwhile, infarct volume, brain water content and neurological deficit score were assessed. The results illustrated that the infarct volume, brain water content and neurofunctional deficiency were significantly reduced by 7days of breviscapine treatment in MCAO+Bre group, compared with those in MCAO+saline group. Meanwhile, the western blotting, quantitative PCR and immunofluorescence showed that the autophagy in both neurons and astrocytes at the penumbra were markedly attenuated by breviscapine admininstration. Moreover, these pharmacological effects of breviscapine could be counteracted by autophagy inducer Tat-Beclin-1. Our study suggests that breviscapine can provide a neuroprotection against transient focal cerebral ischemia, and this biological function is associated with attenuating autophagy in both neurons and astrocytes. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/28343073/Breviscapine_confers_a_neuroprotective_efficacy_against_transient_focal_cerebral_ischemia_by_attenuating_neuronal_and_astrocytic_autophagy_in_the_penumbra_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(16)32759-7 DB - PRIME DP - Unbound Medicine ER -