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Different induction patterns of mRNA for IFN-alpha and -beta in human mononuclear leukocytes after in vitro stimulation with herpes simplex virus-infected fibroblasts and Sendai virus.
J Immunol 1988; 140(10):3605-9JI

Abstract

Human peripheral blood mononuclear leukocytes (PBL) were stimulated in vitro by HSV type 1-infected glutaraldehyde-fixed fibroblasts, or Sendai virus (SV). The PBL containing mRNA for IFN-alpha 2 or -beta 1 were clearly identified by RNA-RNA in situ hybridization by using 35S-labeled alpha 2- and beta 1-probes. Although the two inducers gave similar levels of IFN in the culture medium (about 20 U/10(4) PBL), the patterns of expression of mRNA at the cellular level differed. The HSV induced only IFN-alpha mRNA in the PBL, with a lag of 1 to 2 h, and with a peak frequency of about 10 labeled cells/10(4) PBL at 6 h. Grain counts were high, the majority of cells having more than 50 grains. They were morphologically medium to large lymphocytes. The HSV-infected glutaraldehyde-fixed fibroblasts therefore induce IFN-alpha 2 mRNA in infrequent but highly efficient PBL, each cell capable of producing as much as 2 antiviral units of IFN-alpha. In contrast, SV induced both IFN-alpha 2 and -beta 1 mRNA in PBL, and without clear lags. IFN-beta 1 mRNA-positive PBL peaked somewhat earlier (4 h) than cells containing IFN-alpha 2 mRNA (6 h), and their mean frequencies were approximately 80 and 60/10(4) PBL, respectively, in a panel of PBL from six blood donors. Grain counts were lower than with the HSV inducer, the majority of cells having less than 50 grains, and most labeled cells were morphologically monocytes. The frequency of labeled PBL rapidly decreased with increasing culture time with both the SV and HSV inducers, was low at 12 h and almost absent at 24 h.

Authors+Show Affiliations

Interferon Laboratory, Uppsala University, Sweden.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2834444

Citation

Gobl, A E., et al. "Different Induction Patterns of mRNA for IFN-alpha and -beta in Human Mononuclear Leukocytes After in Vitro Stimulation With Herpes Simplex Virus-infected Fibroblasts and Sendai Virus." Journal of Immunology (Baltimore, Md. : 1950), vol. 140, no. 10, 1988, pp. 3605-9.
Gobl AE, Funa K, Alm GV. Different induction patterns of mRNA for IFN-alpha and -beta in human mononuclear leukocytes after in vitro stimulation with herpes simplex virus-infected fibroblasts and Sendai virus. J Immunol. 1988;140(10):3605-9.
Gobl, A. E., Funa, K., & Alm, G. V. (1988). Different induction patterns of mRNA for IFN-alpha and -beta in human mononuclear leukocytes after in vitro stimulation with herpes simplex virus-infected fibroblasts and Sendai virus. Journal of Immunology (Baltimore, Md. : 1950), 140(10), pp. 3605-9.
Gobl AE, Funa K, Alm GV. Different Induction Patterns of mRNA for IFN-alpha and -beta in Human Mononuclear Leukocytes After in Vitro Stimulation With Herpes Simplex Virus-infected Fibroblasts and Sendai Virus. J Immunol. 1988 May 15;140(10):3605-9. PubMed PMID: 2834444.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Different induction patterns of mRNA for IFN-alpha and -beta in human mononuclear leukocytes after in vitro stimulation with herpes simplex virus-infected fibroblasts and Sendai virus. AU - Gobl,A E, AU - Funa,K, AU - Alm,G V, PY - 1988/5/15/pubmed PY - 1988/5/15/medline PY - 1988/5/15/entrez SP - 3605 EP - 9 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 140 IS - 10 N2 - Human peripheral blood mononuclear leukocytes (PBL) were stimulated in vitro by HSV type 1-infected glutaraldehyde-fixed fibroblasts, or Sendai virus (SV). The PBL containing mRNA for IFN-alpha 2 or -beta 1 were clearly identified by RNA-RNA in situ hybridization by using 35S-labeled alpha 2- and beta 1-probes. Although the two inducers gave similar levels of IFN in the culture medium (about 20 U/10(4) PBL), the patterns of expression of mRNA at the cellular level differed. The HSV induced only IFN-alpha mRNA in the PBL, with a lag of 1 to 2 h, and with a peak frequency of about 10 labeled cells/10(4) PBL at 6 h. Grain counts were high, the majority of cells having more than 50 grains. They were morphologically medium to large lymphocytes. The HSV-infected glutaraldehyde-fixed fibroblasts therefore induce IFN-alpha 2 mRNA in infrequent but highly efficient PBL, each cell capable of producing as much as 2 antiviral units of IFN-alpha. In contrast, SV induced both IFN-alpha 2 and -beta 1 mRNA in PBL, and without clear lags. IFN-beta 1 mRNA-positive PBL peaked somewhat earlier (4 h) than cells containing IFN-alpha 2 mRNA (6 h), and their mean frequencies were approximately 80 and 60/10(4) PBL, respectively, in a panel of PBL from six blood donors. Grain counts were lower than with the HSV inducer, the majority of cells having less than 50 grains, and most labeled cells were morphologically monocytes. The frequency of labeled PBL rapidly decreased with increasing culture time with both the SV and HSV inducers, was low at 12 h and almost absent at 24 h. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2834444/Different_induction_patterns_of_mRNA_for_IFN_alpha_and__beta_in_human_mononuclear_leukocytes_after_in_vitro_stimulation_with_herpes_simplex_virus_infected_fibroblasts_and_Sendai_virus_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=2834444 DB - PRIME DP - Unbound Medicine ER -