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Regulation of gluconeogenic enzymes during the cell cycle of Saccharomyces cerevisiae growing in a chemostat.
J Gen Microbiol. 1987 Sep; 133(9):2517-22.JG

Abstract

Oscillation of the activities of gluconeogenic enzymes (malate dehydrogenase, phosphoenolpyruvate carboxykinase and fructose-1,6-bisphosphatase) was observed during the cell cycle of chemostat cultures of Saccharomyces cerevisiae. Since ethanol is released by the cells at the beginning of the division cycle, its effect on enzyme expression was determined. Pulsing ethanol to a synchronously dividing yeast culture led to a prolongation of the metabolically active phase as indicated by the course of oxygen uptake and carbon dioxide production rates (concomitant ethanol and glucose assimilation). Enzyme activities also remained elevated as long as ethanol was available to the cells. After a substrate shift from glucose to ethanol during cell division, ethanol was used without a lag phase and enzyme induction increased from the level reached at the point of the substrate change. The data confirmed that the small amount of ethanol produced when the cells begin active reproduction acts as an inducer of gluconeogenic enzymes.

Authors+Show Affiliations

Arreguin de Lorencez M
Institute of Biotechnology, Swiss Federal Institute of Technology, Zürich.
Käppeli O
No affiliation info available

MeSH

Cell CycleEthanolFructose-BisphosphataseGluconeogenesisMalate DehydrogenasePhosphoenolpyruvate Carboxykinase (GTP)Saccharomyces cerevisiae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2834508

Citation

Arreguin de Lorencez, M, and O Käppeli. "Regulation of Gluconeogenic Enzymes During the Cell Cycle of Saccharomyces Cerevisiae Growing in a Chemostat." Journal of General Microbiology, vol. 133, no. 9, 1987, pp. 2517-22.
Arreguin de Lorencez M, Käppeli O. Regulation of gluconeogenic enzymes during the cell cycle of Saccharomyces cerevisiae growing in a chemostat. J Gen Microbiol. 1987;133(9):2517-22.
Arreguin de Lorencez, M., & Käppeli, O. (1987). Regulation of gluconeogenic enzymes during the cell cycle of Saccharomyces cerevisiae growing in a chemostat. Journal of General Microbiology, 133(9), 2517-22.
Arreguin de Lorencez M, Käppeli O. Regulation of Gluconeogenic Enzymes During the Cell Cycle of Saccharomyces Cerevisiae Growing in a Chemostat. J Gen Microbiol. 1987;133(9):2517-22. PubMed PMID: 2834508.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of gluconeogenic enzymes during the cell cycle of Saccharomyces cerevisiae growing in a chemostat. AU - Arreguin de Lorencez,M, AU - Käppeli,O, PY - 1987/9/1/pubmed PY - 1987/9/1/medline PY - 1987/9/1/entrez SP - 2517 EP - 22 JF - Journal of general microbiology JO - J Gen Microbiol VL - 133 IS - 9 N2 - Oscillation of the activities of gluconeogenic enzymes (malate dehydrogenase, phosphoenolpyruvate carboxykinase and fructose-1,6-bisphosphatase) was observed during the cell cycle of chemostat cultures of Saccharomyces cerevisiae. Since ethanol is released by the cells at the beginning of the division cycle, its effect on enzyme expression was determined. Pulsing ethanol to a synchronously dividing yeast culture led to a prolongation of the metabolically active phase as indicated by the course of oxygen uptake and carbon dioxide production rates (concomitant ethanol and glucose assimilation). Enzyme activities also remained elevated as long as ethanol was available to the cells. After a substrate shift from glucose to ethanol during cell division, ethanol was used without a lag phase and enzyme induction increased from the level reached at the point of the substrate change. The data confirmed that the small amount of ethanol produced when the cells begin active reproduction acts as an inducer of gluconeogenic enzymes. SN - 0022-1287 UR - https://www.unboundmedicine.com/medline/citation/2834508/Regulation_of_gluconeogenic_enzymes_during_the_cell_cycle_of_Saccharomyces_cerevisiae_growing_in_a_chemostat_ DB - PRIME DP - Unbound Medicine ER -