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Examination of the involvement of supraspinal and spinal mu and delta opioid receptors in analgesia using the mu receptor deficient CXBK mouse.
J Pharmacol Exp Ther. 1988 Apr; 245(1):13-6.JP

Abstract

The role of mu and delta opioid receptors in the spinal and supraspinal analgesic actions of morphine and [D-Pen2, L-Pen5] enkephalin were examined in the tail-flick test utilizing the mu opioid receptor deficient CXBK mouse and BALB/cBy and C57BL/6By, the progenitor strains of CXBK. The analgesic effects of i.c.v. administered morphine were equivalent in the CRS-CD1 (Swiss) standard laboratory mice and the progenitor strains of CXBK. Morphine did not, however, produce analgesia in the CXBK mice at doses greater than 10 times the ED50 dose in the progenitor strains. Similarly, the analgesic effect of i.c.v. [D-Ala2, NMePhe4, Gly-ol]enkephalin, a highly selective mu receptor peptide agonist, also was reduced greatly in the CXBK mice. These data are consistent with the deficiency in mu opioid receptors observed autoradiographically in this strain. In contrast, the highly selective delta opioid receptor peptide agonist [D-Pen2, L-Pen5]enkephalin was equipotent i.c.v. in the CXBK mice and in the progenitor strains of CXBK. In contrast to the effects produced by i.c.v. administration, the analgesic effects of intrathecally administered morphine were similar between CRS-CD1 and CXBX strains of mice. These results suggest that 1) both mu and delta opioid receptors can mediate supraspinal analgesia and 2) that the receptor(s) involved in spinally mediated analgesia is (are) quite distinct from those involved supraspinally.

Authors+Show Affiliations

Department of Biological Research, McNeil Pharmaceutical, Spring House, Pennsylvania.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2834533

Citation

Vaught, J L., et al. "Examination of the Involvement of Supraspinal and Spinal Mu and Delta Opioid Receptors in Analgesia Using the Mu Receptor Deficient CXBK Mouse." The Journal of Pharmacology and Experimental Therapeutics, vol. 245, no. 1, 1988, pp. 13-6.
Vaught JL, Mathiasen JR, Raffa RB. Examination of the involvement of supraspinal and spinal mu and delta opioid receptors in analgesia using the mu receptor deficient CXBK mouse. J Pharmacol Exp Ther. 1988;245(1):13-6.
Vaught, J. L., Mathiasen, J. R., & Raffa, R. B. (1988). Examination of the involvement of supraspinal and spinal mu and delta opioid receptors in analgesia using the mu receptor deficient CXBK mouse. The Journal of Pharmacology and Experimental Therapeutics, 245(1), 13-6.
Vaught JL, Mathiasen JR, Raffa RB. Examination of the Involvement of Supraspinal and Spinal Mu and Delta Opioid Receptors in Analgesia Using the Mu Receptor Deficient CXBK Mouse. J Pharmacol Exp Ther. 1988;245(1):13-6. PubMed PMID: 2834533.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Examination of the involvement of supraspinal and spinal mu and delta opioid receptors in analgesia using the mu receptor deficient CXBK mouse. AU - Vaught,J L, AU - Mathiasen,J R, AU - Raffa,R B, PY - 1988/4/1/pubmed PY - 1988/4/1/medline PY - 1988/4/1/entrez SP - 13 EP - 6 JF - The Journal of pharmacology and experimental therapeutics JO - J Pharmacol Exp Ther VL - 245 IS - 1 N2 - The role of mu and delta opioid receptors in the spinal and supraspinal analgesic actions of morphine and [D-Pen2, L-Pen5] enkephalin were examined in the tail-flick test utilizing the mu opioid receptor deficient CXBK mouse and BALB/cBy and C57BL/6By, the progenitor strains of CXBK. The analgesic effects of i.c.v. administered morphine were equivalent in the CRS-CD1 (Swiss) standard laboratory mice and the progenitor strains of CXBK. Morphine did not, however, produce analgesia in the CXBK mice at doses greater than 10 times the ED50 dose in the progenitor strains. Similarly, the analgesic effect of i.c.v. [D-Ala2, NMePhe4, Gly-ol]enkephalin, a highly selective mu receptor peptide agonist, also was reduced greatly in the CXBK mice. These data are consistent with the deficiency in mu opioid receptors observed autoradiographically in this strain. In contrast, the highly selective delta opioid receptor peptide agonist [D-Pen2, L-Pen5]enkephalin was equipotent i.c.v. in the CXBK mice and in the progenitor strains of CXBK. In contrast to the effects produced by i.c.v. administration, the analgesic effects of intrathecally administered morphine were similar between CRS-CD1 and CXBX strains of mice. These results suggest that 1) both mu and delta opioid receptors can mediate supraspinal analgesia and 2) that the receptor(s) involved in spinally mediated analgesia is (are) quite distinct from those involved supraspinally. SN - 0022-3565 UR - https://www.unboundmedicine.com/medline/citation/2834533/Examination_of_the_involvement_of_supraspinal_and_spinal_mu_and_delta_opioid_receptors_in_analgesia_using_the_mu_receptor_deficient_CXBK_mouse_ DB - PRIME DP - Unbound Medicine ER -