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Relationship between phospholipase C-zeta, semen parameters, and chromatin status.
Syst Biol Reprod Med 2017; 63(4):259-268SB

Abstract

The need for additional tests to complement basic sperm analysis in clinics is well appreciated. In this regard, a number of tests such as sperm DNA integrity test as a tool in diagnosis and treatment of infertility are suggested. But recent studies have focused on main sperm factors involved in oocyte activation such as phospholipase C-zeta (PLCζ) that initiate intracellular Ca2+ signaling and embryogenesis. Therefore, this study aimed to investigate the relationship between PLCζ, basic semen parameters, sperm DNA fragmentation (SDF), and protamine deficiency in men with normal (n=32) and abnormal (n=23) semen parameters. Unlike SDF and protamine deficiency, as negative factors related to fertility, the mean value of PLCζ as positive factor related to infertility was significantly lower in men with abnormal semen parameters compared to men with normal semen parameters. Significant correlations were also observed between sperm concentration, motility, and abnormal morphology with the percentage of PLCζ positive spermatozoa. In addition, logistic regression analysis revealed that sperm morphology is more predictive than sperm motility and concentration for PLCζ presence. In addition, a statistically significant negative relationship was observed between the percentage of PLCζ positive spermatozoa and SDF. These findings suggested during ICSI, selection of sperm based on morphology has a profound effect on its ability to induce oocyte activation based on the likelihood of PLCζ expression. Therefore, assessment of PLCζ as an index for fertilization potential of a semen sample in men with severe teratozoospermia may define individuals who are candidates for artificial oocyte activation (AOA) and may avoid failed fertilization post ICSI.

Authors+Show Affiliations

a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran.a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran.a Department of Reproductive Biotechnology , Reproductive Biomedicine Research Center, Royan Institute for Biotechnology , ACECR , Isfahan , Iran. b Isfahan Fertility and Infertility Center , Isfahan , Iran.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28345968

Citation

Tavalaee, Marziyeh, et al. "Relationship Between Phospholipase C-zeta, Semen Parameters, and Chromatin Status." Systems Biology in Reproductive Medicine, vol. 63, no. 4, 2017, pp. 259-268.
Tavalaee M, Kiani-Esfahani A, Nasr-Esfahani MH. Relationship between phospholipase C-zeta, semen parameters, and chromatin status. Syst Biol Reprod Med. 2017;63(4):259-268.
Tavalaee, M., Kiani-Esfahani, A., & Nasr-Esfahani, M. H. (2017). Relationship between phospholipase C-zeta, semen parameters, and chromatin status. Systems Biology in Reproductive Medicine, 63(4), pp. 259-268. doi:10.1080/19396368.2017.1298006.
Tavalaee M, Kiani-Esfahani A, Nasr-Esfahani MH. Relationship Between Phospholipase C-zeta, Semen Parameters, and Chromatin Status. Syst Biol Reprod Med. 2017;63(4):259-268. PubMed PMID: 28345968.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Relationship between phospholipase C-zeta, semen parameters, and chromatin status. AU - Tavalaee,Marziyeh, AU - Kiani-Esfahani,Abbas, AU - Nasr-Esfahani,Mohammad H, Y1 - 2017/03/27/ PY - 2017/3/28/pubmed PY - 2018/3/27/medline PY - 2017/3/28/entrez KW - DNA fragmentation KW - PLCζ KW - male infertility KW - protamine deficiency KW - semen parameters SP - 259 EP - 268 JF - Systems biology in reproductive medicine JO - Syst Biol Reprod Med VL - 63 IS - 4 N2 - The need for additional tests to complement basic sperm analysis in clinics is well appreciated. In this regard, a number of tests such as sperm DNA integrity test as a tool in diagnosis and treatment of infertility are suggested. But recent studies have focused on main sperm factors involved in oocyte activation such as phospholipase C-zeta (PLCζ) that initiate intracellular Ca2+ signaling and embryogenesis. Therefore, this study aimed to investigate the relationship between PLCζ, basic semen parameters, sperm DNA fragmentation (SDF), and protamine deficiency in men with normal (n=32) and abnormal (n=23) semen parameters. Unlike SDF and protamine deficiency, as negative factors related to fertility, the mean value of PLCζ as positive factor related to infertility was significantly lower in men with abnormal semen parameters compared to men with normal semen parameters. Significant correlations were also observed between sperm concentration, motility, and abnormal morphology with the percentage of PLCζ positive spermatozoa. In addition, logistic regression analysis revealed that sperm morphology is more predictive than sperm motility and concentration for PLCζ presence. In addition, a statistically significant negative relationship was observed between the percentage of PLCζ positive spermatozoa and SDF. These findings suggested during ICSI, selection of sperm based on morphology has a profound effect on its ability to induce oocyte activation based on the likelihood of PLCζ expression. Therefore, assessment of PLCζ as an index for fertilization potential of a semen sample in men with severe teratozoospermia may define individuals who are candidates for artificial oocyte activation (AOA) and may avoid failed fertilization post ICSI. SN - 1939-6376 UR - https://www.unboundmedicine.com/medline/citation/28345968/Relationship_between_phospholipase_C_zeta_semen_parameters_and_chromatin_status_ L2 - http://www.tandfonline.com/doi/full/10.1080/19396368.2017.1298006 DB - PRIME DP - Unbound Medicine ER -