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Cannabinoid CB2 receptors are involved in the protection of RAW264.7 macrophages against the oxidative stress: an in vitro study.
Eur J Histochem 2017; 61(1):2749EJ

Abstract

Research in the last decades has widely investigated the anti-oxidant properties of natural products as a therapeutic approach for the prevention and the treatment of oxidative-stress related disorders. In this context, several studies were aimed to evaluate the therapeutic potential of phytocannabinoids, the bioactive compounds of Cannabis sativa. Here, we examined the anti-oxidant ability of Cannabigerol (CBG), a non-psychotropic cannabinoid, still little known, into counteracting the hydrogen peroxide (H2O2)-induced oxidative stress in murine RAW264.7 macrophages. In addition, we tested selective receptor antagonists for cannabinoid receptors and specifically CB1R (SR141716A) and CB2R (AM630) in order to investigate through which CBG may exert its action. Taken together, our in vitro results showed that CBG is able to counteract oxidative stress by activation of CB2 receptors. CB2 antagonist pre-treatment indeed blocked the protective effects of CBG in H2O2 stimulated macrophages, while CB1R was not involved. Specifically, CBG exhibited a potent action in inhibiting oxidative stress, by down-regulation of the main oxidative markers (iNOS, nitrotyrosine and PARP-1), by preventing IκB-α phosphorylation and translocation of the nuclear factor-κB (NF-κB) and also via the modulation of MAP kinases pathway. On the other hand, CBG was found to increase anti-oxidant defense of cells by modulating superoxide dismutase-1 (SOD-1) expression and thus inhibiting cell death (results focused on balance between Bax and Bcl-2). Based on its antioxidant activities, CBG may hold great promise as an anti-oxidant agent and therefore used in clinical practice as a new approach in oxidative-stress related disorders.

Authors+Show Affiliations

IRCCS Centro Neurolesi "Bonino-Pulejo". giacoppo.sabrina@hotmail.it.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28348416

Citation

Giacoppo, Sabrina, et al. "Cannabinoid CB2 Receptors Are Involved in the Protection of RAW264.7 Macrophages Against the Oxidative Stress: an in Vitro Study." European Journal of Histochemistry : EJH, vol. 61, no. 1, 2017, p. 2749.
Giacoppo S, Gugliandolo A, Trubiani O, et al. Cannabinoid CB2 receptors are involved in the protection of RAW264.7 macrophages against the oxidative stress: an in vitro study. Eur J Histochem. 2017;61(1):2749.
Giacoppo, S., Gugliandolo, A., Trubiani, O., Pollastro, F., Grassi, G., Bramanti, P., & Mazzon, E. (2017). Cannabinoid CB2 receptors are involved in the protection of RAW264.7 macrophages against the oxidative stress: an in vitro study. European Journal of Histochemistry : EJH, 61(1), p. 2749. doi:10.4081/ejh.2017.2749.
Giacoppo S, et al. Cannabinoid CB2 Receptors Are Involved in the Protection of RAW264.7 Macrophages Against the Oxidative Stress: an in Vitro Study. Eur J Histochem. 2017 Jan 23;61(1):2749. PubMed PMID: 28348416.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid CB2 receptors are involved in the protection of RAW264.7 macrophages against the oxidative stress: an in vitro study. AU - Giacoppo,Sabrina, AU - Gugliandolo,Agnese, AU - Trubiani,Oriana, AU - Pollastro,Federica, AU - Grassi,Gianpaolo, AU - Bramanti,Placido, AU - Mazzon,Emanuela, Y1 - 2017/01/23/ PY - 2016/11/03/received PY - 2016/12/21/accepted PY - 2016/12/20/revised PY - 2017/3/29/entrez PY - 2017/3/30/pubmed PY - 2017/5/16/medline SP - 2749 EP - 2749 JF - European journal of histochemistry : EJH JO - Eur J Histochem VL - 61 IS - 1 N2 - Research in the last decades has widely investigated the anti-oxidant properties of natural products as a therapeutic approach for the prevention and the treatment of oxidative-stress related disorders. In this context, several studies were aimed to evaluate the therapeutic potential of phytocannabinoids, the bioactive compounds of Cannabis sativa. Here, we examined the anti-oxidant ability of Cannabigerol (CBG), a non-psychotropic cannabinoid, still little known, into counteracting the hydrogen peroxide (H2O2)-induced oxidative stress in murine RAW264.7 macrophages. In addition, we tested selective receptor antagonists for cannabinoid receptors and specifically CB1R (SR141716A) and CB2R (AM630) in order to investigate through which CBG may exert its action. Taken together, our in vitro results showed that CBG is able to counteract oxidative stress by activation of CB2 receptors. CB2 antagonist pre-treatment indeed blocked the protective effects of CBG in H2O2 stimulated macrophages, while CB1R was not involved. Specifically, CBG exhibited a potent action in inhibiting oxidative stress, by down-regulation of the main oxidative markers (iNOS, nitrotyrosine and PARP-1), by preventing IκB-α phosphorylation and translocation of the nuclear factor-κB (NF-κB) and also via the modulation of MAP kinases pathway. On the other hand, CBG was found to increase anti-oxidant defense of cells by modulating superoxide dismutase-1 (SOD-1) expression and thus inhibiting cell death (results focused on balance between Bax and Bcl-2). Based on its antioxidant activities, CBG may hold great promise as an anti-oxidant agent and therefore used in clinical practice as a new approach in oxidative-stress related disorders. SN - 2038-8306 UR - https://www.unboundmedicine.com/medline/citation/28348416/Cannabinoid_CB2_receptors_are_involved_in_the_protection_of_RAW264_7_macrophages_against_the_oxidative_stress:_an_in_vitro_study_ L2 - https://doi.org10.4081/ejh.2017.2749 DB - PRIME DP - Unbound Medicine ER -