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Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China.
Emerg Microbes Infect. 2017 Mar 29; 6(3):e14.EM

Abstract

To investigate bat coronaviruses (CoVs), we collected 132 rectal swabs and urine samples from five bat species in three countries in southwestern China. Seven CoVs belonging to distinct groups of severe acute respiratory syndrome (SARS)-like CoVs and α-CoVs were detected in samples from least horseshoe bats. Samples from other bat species were negative for these viruses, indicating that the least horseshoe bat represents one of the natural reservoirs and mixers for strains of CoVs and has a pivotal role in the evolution and dissemination of these viruses. The genetic and evolutionary characteristics of these strains were described. Whole-genome sequencing of a new isolate (F46) from a rectal swab from a least horseshoe bat showed that it contained 29 699 nucleotides, excluding the poly (A) tail, with 13 open reading frames (ORFs). Phylogenetic and recombination analyses of F46 provided evidence of natural recombination between bat SARS-like CoVs (Rs3367 and LYRa11) or SARS-CoV (BJ01), suggesting that F46 could be a new recombinant virus from SARS-like CoVs or SARS-CoVs.

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Authors+Show Affiliations

Wang L
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
Fu S
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
Cao Y
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
Zhang H
Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China.
Feng Y
Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China.
Yang W
Yunnan Institute of Endemic Diseases Control and Prevention, Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention of Dali, Dali 671000, Yunnan, People's Republic of China.
Nie K
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
Ma X
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.
Liang G
State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, People's Republic of China. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, People's Republic of China.

MeSH

AnimalsChiropteraCoronavirusEvolution, MolecularGenome SizeGenome, ViralOpen Reading FramesPhylogenyRNA, ViralRecombination, GeneticRectumUrine

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28352124

Citation

Wang, Lihua, et al. "Discovery and Genetic Analysis of Novel Coronaviruses in Least Horseshoe Bats in Southwestern China." Emerging Microbes & Infections, vol. 6, no. 3, 2017, pp. e14.
Wang L, Fu S, Cao Y, et al. Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China. Emerg Microbes Infect. 2017;6(3):e14.
Wang, L., Fu, S., Cao, Y., Zhang, H., Feng, Y., Yang, W., Nie, K., Ma, X., & Liang, G. (2017). Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China. Emerging Microbes & Infections, 6(3), e14. https://doi.org/10.1038/emi.2016.140
Wang L, et al. Discovery and Genetic Analysis of Novel Coronaviruses in Least Horseshoe Bats in Southwestern China. Emerg Microbes Infect. 2017 Mar 29;6(3):e14. PubMed PMID: 28352124.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Discovery and genetic analysis of novel coronaviruses in least horseshoe bats in southwestern China. AU - Wang,Lihua, AU - Fu,Shihong, AU - Cao,Yuxi, AU - Zhang,Hailin, AU - Feng,Yun, AU - Yang,Weihong, AU - Nie,Kai, AU - Ma,Xuejun, AU - Liang,Guodong, Y1 - 2017/03/29/ PY - 2016/08/23/received PY - 2016/12/21/revised PY - 2016/12/27/accepted PY - 2017/3/30/entrez PY - 2017/3/30/pubmed PY - 2017/4/7/medline SP - e14 EP - e14 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 6 IS - 3 N2 - To investigate bat coronaviruses (CoVs), we collected 132 rectal swabs and urine samples from five bat species in three countries in southwestern China. Seven CoVs belonging to distinct groups of severe acute respiratory syndrome (SARS)-like CoVs and α-CoVs were detected in samples from least horseshoe bats. Samples from other bat species were negative for these viruses, indicating that the least horseshoe bat represents one of the natural reservoirs and mixers for strains of CoVs and has a pivotal role in the evolution and dissemination of these viruses. The genetic and evolutionary characteristics of these strains were described. Whole-genome sequencing of a new isolate (F46) from a rectal swab from a least horseshoe bat showed that it contained 29 699 nucleotides, excluding the poly (A) tail, with 13 open reading frames (ORFs). Phylogenetic and recombination analyses of F46 provided evidence of natural recombination between bat SARS-like CoVs (Rs3367 and LYRa11) or SARS-CoV (BJ01), suggesting that F46 could be a new recombinant virus from SARS-like CoVs or SARS-CoVs. SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/28352124/Discovery_and_genetic_analysis_of_novel_coronaviruses_in_least_horseshoe_bats_in_southwestern_China_ L2 - https://www.tandfonline.com/doi/full/10.1038/emi.2016.140 DB - PRIME DP - Unbound Medicine ER -