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Enrichment of Triglyceride-Rich Lipoproteins with Apolipoprotein C-I Is Positively Associated with Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women.
J Nutr. 2017 05; 147(5):754-762.JN

Abstract

Background:

The role of plasma apolipoprotein (apo) C-I in cardiometabolic risk in humans is unclear. However, in vitro studies showed a dual role for apoC-I, both protective and harmful, depending on the carrier lipoprotein.

Objective:

We tested the hypothesis that triglyceride (TG)-rich lipoprotein (TRL) apoC-I, not total or HDL apoC-I, is associated with delayed postprandial plasma clearance of TRLs, independently of apoC-II, apoC-III, and apoE.

Methods:

This cross-sectional study examines the plasma clearance of a 13C-triolein-labeled high-fat meal (68% fat energy) in 20 postmenopausal overweight and obese women [body mass index (in kg/m2) ≥27; aged 45-74 y] as the increment change in area under the 6-h postprandial curves (iAUC6h) of TRL parameters. Lipoproteins were fractionated by fast-protein LC. Transferable apolipoproteins were measured by ELISA. TRL enrichment with apolipoproteins was calculated by dividing their TRL concentrations by TRL apoB. The effects of human apoC-I and apoC-III on the hydrolysis and storage of 3H-triolein-labeled TRLs were tested in 3T3-L1 adipocytes.

Results:

TRL apoC-I was positively associated with plasma apo B-48 and total and non-HDL TGs, cholesterol, and apoB (r = 0.52-0.97) and negatively with HDL cholesterol (r = -0.52) and LDL diameter (r = -0.91) (P < 0.05). Total and HDL apoC-I were correlated only with total (r = 0.62) and HDL (r = 0.75) cholesterol. Women with high fasting TRL enrichment with apoC-I (99-365 μmol apoC-I/μmol apoB), but not apoC-II, apoC-III, or apoE, had higher iAUC6h for TGs (+195%), 13C-TGs (+319%), and apo B-48 (+186%) than those with low enrichment (14-97 μmol apoC-I/μmol apoB). The 4-h postprandial increase in TRL apoC-I was associated with a 4-h increase in TRL TGs and iAUC6h for TGs, 13C-TGs, and apo B-48 (r = 0.74-0.86, P < 0.001), independently of 4-h changes in TRL apoB, apoC-II, apoC-III, or apoE. ApoC-I and apoC-III inhibited 3H-TRL clearance by adipocytes by >75% (P < 0.001).

Conclusions:

TRL enrichment with apoC-I is positively associated with postprandial hypertriglyceridemia and remnant accumulation in postmenopausal overweight and obese women, independently of apoC-II, apoC-III, or apoE, which may be due to inhibiting TRL clearance by adipocytes. Reducing TRL apoC-I may ameliorate delayed postprandial plasma clearance of TRLs and associated risks in humans.

Authors+Show Affiliations

Montreal Clinical Research Institute, Montreal, Quebec, Canada. Faculty of Medicine, University of Montreal, Montreal, Quebec, Canada; and. Montreal Diabetes Research Center, Montreal, Quebec, Canada.Montreal Clinical Research Institute, Montreal, Quebec, Canada. Faculty of Medicine, University of Montreal, Montreal, Quebec, Canada; and. Montreal Diabetes Research Center, Montreal, Quebec, Canada.Montreal Clinical Research Institute, Montreal, Quebec, Canada. Faculty of Medicine, University of Montreal, Montreal, Quebec, Canada; and.Montreal Clinical Research Institute, Montreal, Quebec, Canada. Faculty of Medicine, University of Montreal, Montreal, Quebec, Canada; and.Montreal Clinical Research Institute, Montreal, Quebec, Canada; may.faraj@umontreal.ca. Faculty of Medicine, University of Montreal, Montreal, Quebec, Canada; and. Montreal Diabetes Research Center, Montreal, Quebec, Canada.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28356429

Citation

Wassef, Hanny, et al. "Enrichment of Triglyceride-Rich Lipoproteins With Apolipoprotein C-I Is Positively Associated With Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women." The Journal of Nutrition, vol. 147, no. 5, 2017, pp. 754-762.
Wassef H, Bissonnette S, Dufour R, et al. Enrichment of Triglyceride-Rich Lipoproteins with Apolipoprotein C-I Is Positively Associated with Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women. J Nutr. 2017;147(5):754-762.
Wassef, H., Bissonnette, S., Dufour, R., Davignon, J., & Faraj, M. (2017). Enrichment of Triglyceride-Rich Lipoproteins with Apolipoprotein C-I Is Positively Associated with Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women. The Journal of Nutrition, 147(5), 754-762. https://doi.org/10.3945/jn.116.242750
Wassef H, et al. Enrichment of Triglyceride-Rich Lipoproteins With Apolipoprotein C-I Is Positively Associated With Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women. J Nutr. 2017;147(5):754-762. PubMed PMID: 28356429.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enrichment of Triglyceride-Rich Lipoproteins with Apolipoprotein C-I Is Positively Associated with Their Delayed Plasma Clearance Independently of Other Transferable Apolipoproteins in Postmenopausal Overweight and Obese Women. AU - Wassef,Hanny, AU - Bissonnette,Simon, AU - Dufour,Robert, AU - Davignon,Jean, AU - Faraj,May, Y1 - 2017/03/29/ PY - 2016/10/12/received PY - 2016/11/23/revised PY - 2017/03/02/accepted PY - 2017/3/31/pubmed PY - 2017/7/20/medline PY - 2017/3/31/entrez KW - apoB lipoproteins KW - chylomicron and VLDL clearance KW - in situ LPL activity KW - overweight and obese postmenopausal women KW - remnants SP - 754 EP - 762 JF - The Journal of nutrition JO - J. Nutr. VL - 147 IS - 5 N2 - Background: The role of plasma apolipoprotein (apo) C-I in cardiometabolic risk in humans is unclear. However, in vitro studies showed a dual role for apoC-I, both protective and harmful, depending on the carrier lipoprotein.Objective: We tested the hypothesis that triglyceride (TG)-rich lipoprotein (TRL) apoC-I, not total or HDL apoC-I, is associated with delayed postprandial plasma clearance of TRLs, independently of apoC-II, apoC-III, and apoE.Methods: This cross-sectional study examines the plasma clearance of a 13C-triolein-labeled high-fat meal (68% fat energy) in 20 postmenopausal overweight and obese women [body mass index (in kg/m2) ≥27; aged 45-74 y] as the increment change in area under the 6-h postprandial curves (iAUC6h) of TRL parameters. Lipoproteins were fractionated by fast-protein LC. Transferable apolipoproteins were measured by ELISA. TRL enrichment with apolipoproteins was calculated by dividing their TRL concentrations by TRL apoB. The effects of human apoC-I and apoC-III on the hydrolysis and storage of 3H-triolein-labeled TRLs were tested in 3T3-L1 adipocytes.Results: TRL apoC-I was positively associated with plasma apo B-48 and total and non-HDL TGs, cholesterol, and apoB (r = 0.52-0.97) and negatively with HDL cholesterol (r = -0.52) and LDL diameter (r = -0.91) (P < 0.05). Total and HDL apoC-I were correlated only with total (r = 0.62) and HDL (r = 0.75) cholesterol. Women with high fasting TRL enrichment with apoC-I (99-365 μmol apoC-I/μmol apoB), but not apoC-II, apoC-III, or apoE, had higher iAUC6h for TGs (+195%), 13C-TGs (+319%), and apo B-48 (+186%) than those with low enrichment (14-97 μmol apoC-I/μmol apoB). The 4-h postprandial increase in TRL apoC-I was associated with a 4-h increase in TRL TGs and iAUC6h for TGs, 13C-TGs, and apo B-48 (r = 0.74-0.86, P < 0.001), independently of 4-h changes in TRL apoB, apoC-II, apoC-III, or apoE. ApoC-I and apoC-III inhibited 3H-TRL clearance by adipocytes by >75% (P < 0.001).Conclusions: TRL enrichment with apoC-I is positively associated with postprandial hypertriglyceridemia and remnant accumulation in postmenopausal overweight and obese women, independently of apoC-II, apoC-III, or apoE, which may be due to inhibiting TRL clearance by adipocytes. Reducing TRL apoC-I may ameliorate delayed postprandial plasma clearance of TRLs and associated risks in humans. SN - 1541-6100 UR - https://www.unboundmedicine.com/medline/citation/28356429/Enrichment_of_Triglyceride_Rich_Lipoproteins_with_Apolipoprotein_C_I_Is_Positively_Associated_with_Their_Delayed_Plasma_Clearance_Independently_of_Other_Transferable_Apolipoproteins_in_Postmenopausal_Overweight_and_Obese_Women_ L2 - https://academic.oup.com/jn/article-lookup/doi/10.3945/jn.116.242750 DB - PRIME DP - Unbound Medicine ER -