Citation
Wang, Haichuan, et al. "Patterns of Gene Expression in Western Corn Rootworm (Diabrotica Virgifera Virgifera) Neonates, Challenged With Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based On Next-Generation Sequencing." Toxins, vol. 9, no. 4, 2017.
Wang H, Eyun SI, Arora K, et al. Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing. Toxins (Basel). 2017;9(4).
Wang, H., Eyun, S. I., Arora, K., Tan, S. Y., Gandra, P., Moriyama, E., Khajuria, C., Jurzenski, J., Li, H., Donahue, M., Narva, K., & Siegfried, B. (2017). Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing. Toxins, 9(4). https://doi.org/10.3390/toxins9040124
Wang H, et al. Patterns of Gene Expression in Western Corn Rootworm (Diabrotica Virgifera Virgifera) Neonates, Challenged With Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based On Next-Generation Sequencing. Toxins (Basel). 2017 03 30;9(4) PubMed PMID: 28358336.
TY - JOUR
T1 - Patterns of Gene Expression in Western Corn Rootworm (Diabrotica virgifera virgifera) Neonates, Challenged with Cry34Ab1, Cry35Ab1 and Cry34/35Ab1, Based on Next-Generation Sequencing.
AU - Wang,Haichuan,
AU - Eyun,Seong-Il,
AU - Arora,Kanika,
AU - Tan,Sek Yee,
AU - Gandra,Premchand,
AU - Moriyama,Etsuko,
AU - Khajuria,Chitvan,
AU - Jurzenski,Jessica,
AU - Li,Huarong,
AU - Donahue,Maia,
AU - Narva,Ken,
AU - Siegfried,Blair,
Y1 - 2017/03/30/
PY - 2017/02/26/received
PY - 2017/03/22/revised
PY - 2017/03/27/accepted
PY - 2017/3/31/entrez
PY - 2017/3/31/pubmed
PY - 2017/11/10/medline
KW - Bt challenge
KW - Diabrotica virgifera virgifera
KW - Next Generation Sequencing (NGS)
KW - differential gene expression
JF - Toxins
JO - Toxins (Basel)
VL - 9
IS - 4
N2 - With Next Generation Sequencing technologies, high-throughput RNA sequencing (RNAseq) was conducted to examine gene expression in neonates of Diabrotica virgifera virgifera (LeConte) (Western Corn Rootworm, WCR) challenged with individual proteins of the binary Bacillus thuringiensis insecticidal proteins, Cry34Ab1 and Cry35Ab1, and the combination of Cry34/Cry35Ab1, which together are active against rootworm larvae. Integrated results of three different statistical comparisons identified 114 and 1300 differentially expressed transcripts (DETs) in the Cry34Ab1 and Cry34/35Ab1 treatment, respectively, as compared to the control. No DETs were identified in the Cry35Ab1 treatment. Putative Bt binding receptors previously identified in other insect species were not identified in DETs in this study. The majority of DETs (75% with Cry34Ab1 and 68.3% with Cry34/35Ab1 treatments) had no significant hits in the NCBI nr database. In addition, 92 DETs were shared between Cry34Ab1 and Cry34/35Ab1 treatments. Further analysis revealed that the most abundant DETs in both Cry34Ab1 and Cry34/35Ab1 treatments were associated with binding and catalytic activity. Results from this study confirmed the nature of these binary toxins against WCR larvae and provide a fundamental profile of expression pattern of genes in response to challenge of the Cry34/35Ab1 toxin, which may provide insight into potential resistance mechanisms.
SN - 2072-6651
UR - https://www.unboundmedicine.com/medline/citation/28358336/Patterns_of_Gene_Expression_in_Western_Corn_Rootworm__Diabrotica_virgifera_virgifera__Neonates_Challenged_with_Cry34Ab1_Cry35Ab1_and_Cry34/35Ab1_Based_on_Next_Generation_Sequencing_
L2 - https://www.mdpi.com/resolver?pii=toxins9040124
DB - PRIME
DP - Unbound Medicine
ER -
