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Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM).
Braz J Microbiol. 2017 Jul - Sep; 48(3):427-441.BJ

Abstract

The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses. The pH and temperature value of the cultivation medium were the most significant variables and the effects of inoculum size and agitation speed were slightly lower. The intra-extracellular inulinase, invertase production and biomass content increased 10-32 fold in the optimized medium condition (pH 6.5, temperature 30°C, 6% (v/v), inoculum size and 150rpm agitation speed) by RSM compared with medium optimized through the one-factor-at-a-time method. The process development and intensification for simultaneous production of intra-extracellular inulinase (exo and endo inulinase) and invertase from A. niger could be used for industrial applications.

Authors+Show Affiliations

The University of Sydney, School of Chemistry, New South Wales, Australia. Electronic address: mojdeh.dinarvand@sydney.edu.au.Islamic Azad University, Falavarjan Branch, Department of Biochemistry, Isfahan, Iran.Universiti Putra Malaysia, Faculty of Biotechnology and Biomolecular Science, Department of Cell and Molecular Biology, Selangor, Malaysia.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

28359854

Citation

Dinarvand, Mojdeh, et al. "Optimizing Culture Conditions for Production of Intra and Extracellular Inulinase and Invertase From Aspergillus Niger ATCC 20611 By Response Surface Methodology (RSM)." Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], vol. 48, no. 3, 2017, pp. 427-441.
Dinarvand M, Rezaee M, Foroughi M. Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM). Braz J Microbiol. 2017;48(3):427-441.
Dinarvand, M., Rezaee, M., & Foroughi, M. (2017). Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM). Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology], 48(3), 427-441. https://doi.org/10.1016/j.bjm.2016.10.026
Dinarvand M, Rezaee M, Foroughi M. Optimizing Culture Conditions for Production of Intra and Extracellular Inulinase and Invertase From Aspergillus Niger ATCC 20611 By Response Surface Methodology (RSM). Braz J Microbiol. 2017 Jul - Sep;48(3):427-441. PubMed PMID: 28359854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimizing culture conditions for production of intra and extracellular inulinase and invertase from Aspergillus niger ATCC 20611 by response surface methodology (RSM). AU - Dinarvand,Mojdeh, AU - Rezaee,Malahat, AU - Foroughi,Majid, Y1 - 2017/02/10/ PY - 2016/04/30/received PY - 2016/09/13/revised PY - 2016/10/16/accepted PY - 2017/4/1/pubmed PY - 2018/3/13/medline PY - 2017/4/1/entrez KW - Aspergillus niger KW - Intra-extracellular inulinase production KW - Intra-extracellular invertase production KW - Optimization KW - Response surface method (RSM) SP - 427 EP - 441 JF - Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] JO - Braz. J. Microbiol. VL - 48 IS - 3 N2 - The aim of this study was obtain a model that maximizes growth and production of inulinase and invertase by Aspergillus niger ATCC 20611, employing response surface methodology (RSM). The RSM with a five-variable and three-level central composite design (CCD) was employed to optimize the medium composition. Results showed that the experimental data could be appropriately fitted into a second-order polynomial model with a coefficient of determination (R2) more than 0.90 for all responses. This model adequately explained the data variation and represented the actual relationships between the parameters and responses. The pH and temperature value of the cultivation medium were the most significant variables and the effects of inoculum size and agitation speed were slightly lower. The intra-extracellular inulinase, invertase production and biomass content increased 10-32 fold in the optimized medium condition (pH 6.5, temperature 30°C, 6% (v/v), inoculum size and 150rpm agitation speed) by RSM compared with medium optimized through the one-factor-at-a-time method. The process development and intensification for simultaneous production of intra-extracellular inulinase (exo and endo inulinase) and invertase from A. niger could be used for industrial applications. SN - 1678-4405 UR - https://www.unboundmedicine.com/medline/citation/28359854/Optimizing_culture_conditions_for_production_of_intra_and_extracellular_inulinase_and_invertase_from_Aspergillus_niger_ATCC_20611_by_response_surface_methodology__RSM__ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/28359854/ DB - PRIME DP - Unbound Medicine ER -