Comparison of the effects of commercial coated and uncoated ZnO nanomaterials and Zn compounds in kidney bean (Phaseolus vulgaris) plants.J Hazard Mater. 2017 Jun 15; 332:214-222.JH
Bean (Phaseolus vulgaris) plants were grown for 45 days in soil amended with either uncoated (Z-COTE®) and coated (Z-COTE HP1®) ZnO nanomaterials (NMs), bulk ZnO and ZnCl2, at 0-500mg/kg. At harvest, growth parameters, chlorophyll, and essential elements were determined. None of the treatments affected germination and pod production, and only ZnCl2 at 250 and 500mg/kg reduced relative chlorophyll content by 34% and 46%, respectively. While Z-COTE® did not produce phenotypic changes, Z-COTE HP1®, at all concentrations, increased root length (∼44%) and leaf length (∼13%) compared with control. Bulk ZnO reduced root length (53%) at 62.5mg/kg and ZnCl2 reduced leaf length (16%) at 125mg/kg. Z-COTE®, at 125mg/kg, increased Zn by 203%, 139%, and 76% in nodules, stems, and leaves, respectively; while at the same concentration, Z-COTE HP1® increased Zn by 89%, 97%, and 103% in roots, stems, and leaves, respectively. At 125mg/kg, Z-COTE HP1® increased root S (65%) and Mg (65%), while Z-COTE® increased stem B (122%) and Mn (73%). Bulk ZnO and ZnCl2 imposed more toxicity to kidney bean than the NMs, since they reduced root and leaf elongation, respectively, and the concentration of several essential elements in tissues.