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Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.
J Bacteriol. 1988 Jun; 170(6):2763-9.JB

Abstract

In Escherichia coli, the phosphorylation and dephosphorylation of isocitrate dehydrogenase (IDH) are catalyzed by a bifunctional protein kinase/phosphatase. We have determined the nucleotide sequence of aceK, the gene encoding IDH kinase/phosphatase. This gene consists of a single open reading frame of 1,734 base pairs preceded by a Shine-Dalgarno ribosome-binding site. Examination of the deduced amino acid sequence of IDH kinase/phosphatase revealed sequences which are similar to the consensus sequence for ATP-binding sites. This protein did not, however, exhibit the extensive sequence homologies which are typical of other protein kinases. Multiple copies of the REP family of repetitive extragenic elements were found within the intergenic region between aceA (encoding isocitrate lyase) and aceK. These elements have the potential for combining to form an exceptionally stable stem-loop structure (delta G = -54 kcal/mol [ca. -226 kJ/mol]) in the mRNA. This structure, which masks the ribosome-binding site and start codon for aceK, may contribute to the downshift in expression observed between aceA and aceK. Another potential stem-loop structure (delta G = -29 kcal/mol [ca. 121 kJ/mol]), unrelated to the REP sequences, was found within aceK.

Authors+Show Affiliations

Department of Biochemistry, University of Minnesota, Minneapolis 55455.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2836370

Citation

Klumpp, D J., et al. "Nucleotide Sequence of aceK, the Gene Encoding Isocitrate Dehydrogenase Kinase/phosphatase." Journal of Bacteriology, vol. 170, no. 6, 1988, pp. 2763-9.
Klumpp DJ, Plank DW, Bowdin LJ, et al. Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase. J Bacteriol. 1988;170(6):2763-9.
Klumpp, D. J., Plank, D. W., Bowdin, L. J., Stueland, C. S., Chung, T., & LaPorte, D. C. (1988). Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase. Journal of Bacteriology, 170(6), 2763-9.
Klumpp DJ, et al. Nucleotide Sequence of aceK, the Gene Encoding Isocitrate Dehydrogenase Kinase/phosphatase. J Bacteriol. 1988;170(6):2763-9. PubMed PMID: 2836370.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase. AU - Klumpp,D J, AU - Plank,D W, AU - Bowdin,L J, AU - Stueland,C S, AU - Chung,T, AU - LaPorte,D C, PY - 1988/6/1/pubmed PY - 1988/6/1/medline PY - 1988/6/1/entrez SP - 2763 EP - 9 JF - Journal of bacteriology JO - J Bacteriol VL - 170 IS - 6 N2 - In Escherichia coli, the phosphorylation and dephosphorylation of isocitrate dehydrogenase (IDH) are catalyzed by a bifunctional protein kinase/phosphatase. We have determined the nucleotide sequence of aceK, the gene encoding IDH kinase/phosphatase. This gene consists of a single open reading frame of 1,734 base pairs preceded by a Shine-Dalgarno ribosome-binding site. Examination of the deduced amino acid sequence of IDH kinase/phosphatase revealed sequences which are similar to the consensus sequence for ATP-binding sites. This protein did not, however, exhibit the extensive sequence homologies which are typical of other protein kinases. Multiple copies of the REP family of repetitive extragenic elements were found within the intergenic region between aceA (encoding isocitrate lyase) and aceK. These elements have the potential for combining to form an exceptionally stable stem-loop structure (delta G = -54 kcal/mol [ca. -226 kJ/mol]) in the mRNA. This structure, which masks the ribosome-binding site and start codon for aceK, may contribute to the downshift in expression observed between aceA and aceK. Another potential stem-loop structure (delta G = -29 kcal/mol [ca. 121 kJ/mol]), unrelated to the REP sequences, was found within aceK. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/2836370/Nucleotide_sequence_of_aceK_the_gene_encoding_isocitrate_dehydrogenase_kinase/phosphatase_ L2 - https://journals.asm.org/doi/10.1128/jb.170.6.2763-2769.1988?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -