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Evaluation of an enzyme-linked immunosorbent assay for quantitation of antibodies to Junin virus in human sera.
J Virol Methods. 1988 Mar-Apr; 19(3-4):299-305.JV

Abstract

An enzyme-linked immunosorbent assay (ELISA) was evaluated for the quantitation of anti-Junin virus (JV) antibodies, in 83 selected cases of Argentine haemorrhagic fever (AHF). Serum samples were studied in two groups to facilitate comparative analysis; the first group was ELISA with indirect immunofluorescence (IF) test, in the second ELISA with plaque reduction neutralization test (PRINT). From the results obtained by using ELISA and IF on the same serum samples, a clear tendency of ELISA to demonstrate seroconversion for JV earlier and at higher frequency than IF test was noted. Simultaneous titration of specific antibodies by ELISA and PRNT tests rendered significantly correlated titers (r = 0.81), both methods being equivalently specific (100%). The demonstration of specific antibodies by ELISA in two cases that were undetected by the PRNT test resulted in a higher sensitivity index for ELISA than for PRNT (100% vs 97%). It is concluded that ELISA could efficiently replace IF and PRNT tests for the diagnosis of AHF.

Authors+Show Affiliations

Instituto Nacional de Estudios sobre Virosis Hemorrágicas, Pergamino, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2836465

Citation

García Franco, S, et al. "Evaluation of an Enzyme-linked Immunosorbent Assay for Quantitation of Antibodies to Junin Virus in Human Sera." Journal of Virological Methods, vol. 19, no. 3-4, 1988, pp. 299-305.
García Franco S, Ambrosio AM, Feuillade MR, et al. Evaluation of an enzyme-linked immunosorbent assay for quantitation of antibodies to Junin virus in human sera. J Virol Methods. 1988;19(3-4):299-305.
García Franco, S., Ambrosio, A. M., Feuillade, M. R., & Maiztegui, J. I. (1988). Evaluation of an enzyme-linked immunosorbent assay for quantitation of antibodies to Junin virus in human sera. Journal of Virological Methods, 19(3-4), 299-305.
García Franco S, et al. Evaluation of an Enzyme-linked Immunosorbent Assay for Quantitation of Antibodies to Junin Virus in Human Sera. J Virol Methods. 1988 Mar-Apr;19(3-4):299-305. PubMed PMID: 2836465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of an enzyme-linked immunosorbent assay for quantitation of antibodies to Junin virus in human sera. AU - García Franco,S, AU - Ambrosio,A M, AU - Feuillade,M R, AU - Maiztegui,J I, PY - 1988/3/1/pubmed PY - 1988/3/1/medline PY - 1988/3/1/entrez SP - 299 EP - 305 JF - Journal of virological methods JO - J Virol Methods VL - 19 IS - 3-4 N2 - An enzyme-linked immunosorbent assay (ELISA) was evaluated for the quantitation of anti-Junin virus (JV) antibodies, in 83 selected cases of Argentine haemorrhagic fever (AHF). Serum samples were studied in two groups to facilitate comparative analysis; the first group was ELISA with indirect immunofluorescence (IF) test, in the second ELISA with plaque reduction neutralization test (PRINT). From the results obtained by using ELISA and IF on the same serum samples, a clear tendency of ELISA to demonstrate seroconversion for JV earlier and at higher frequency than IF test was noted. Simultaneous titration of specific antibodies by ELISA and PRNT tests rendered significantly correlated titers (r = 0.81), both methods being equivalently specific (100%). The demonstration of specific antibodies by ELISA in two cases that were undetected by the PRNT test resulted in a higher sensitivity index for ELISA than for PRNT (100% vs 97%). It is concluded that ELISA could efficiently replace IF and PRNT tests for the diagnosis of AHF. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/2836465/Evaluation_of_an_enzyme_linked_immunosorbent_assay_for_quantitation_of_antibodies_to_Junin_virus_in_human_sera_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0166-0934(88)90024-9 DB - PRIME DP - Unbound Medicine ER -