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Suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, Nilaparvata lugens.
Pestic Biochem Physiol 2017; 137:81-90PB

Abstract

Trehalase (TRE) is a key enzyme in trehalose degradation and has important functions in insect growth and chitin synthesis. Though validamycin has the potential for pest control by suppressing TRE activities, it is not known whether validamycin acts on both trehalose and chitin metabolism. TRE1 and TRE2 activities and glucose and glycogen contents decreased significantly after the injection of different doses of validamycin solution compared with the control group, while the trehalose content increased significantly. Overall, it showed that about 13 to 38% insects was appeared abnormal phenotypes, and 10 to 57% of insects died 48h after injection of solutions with different concentrations of validamycin; the chitin content also decreased significantly. Validamycin altered the relative expression levels of trehalose, glycogen and chitin metabolism-related genes by suppressing the activities of two TREs. We showed that the expression levels of three TRE and two trehalose-6-phosphate synthase (TPS) genes increased, while the expression levels of GP; CHS1 and its two transcripts, CHS1a, CHS1b; six chitinases, including Cht3, Cht4, Cht5, Cht6, Cht7, Cht9; and the HK, G6PI2, GFAT, GNPNA, PAGM1, UAP, VVL, CI and AP genes decreased significantly 48h after the injection of any validamycin concentration compared with the control group. These results demonstrate that by inhibiting the activities of two TREs, validamycin alters N. lugens chitin synthesis and degradation and affects trehalose and chitin metabolism-related gene expression. The development of TRE inhibitors may provide effective pest control in the future.

Authors+Show Affiliations

Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang 310036, China.Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang 310036, China.Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang 310036, China.Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang 310036, China.Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang 310036, China.Hangzhou Key Laboratory of Animal Adaptation and Evolution, College of Life and Environmental Sciences, Hangzhou Normal University, Zhejiang 310036, China. Electronic address: sgwang@mail.hz.zj.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28364808

Citation

Tang, Bin, et al. "Suppressing the Activity of Trehalase With Validamycin Disrupts the Trehalose and Chitin Biosynthesis Pathways in the Rice Brown Planthopper, Nilaparvata Lugens." Pesticide Biochemistry and Physiology, vol. 137, 2017, pp. 81-90.
Tang B, Yang M, Shen Q, et al. Suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, Nilaparvata lugens. Pestic Biochem Physiol. 2017;137:81-90.
Tang, B., Yang, M., Shen, Q., Xu, Y., Wang, H., & Wang, S. (2017). Suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, Nilaparvata lugens. Pesticide Biochemistry and Physiology, 137, pp. 81-90. doi:10.1016/j.pestbp.2016.10.003.
Tang B, et al. Suppressing the Activity of Trehalase With Validamycin Disrupts the Trehalose and Chitin Biosynthesis Pathways in the Rice Brown Planthopper, Nilaparvata Lugens. Pestic Biochem Physiol. 2017;137:81-90. PubMed PMID: 28364808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppressing the activity of trehalase with validamycin disrupts the trehalose and chitin biosynthesis pathways in the rice brown planthopper, Nilaparvata lugens. AU - Tang,Bin, AU - Yang,Mengmeng, AU - Shen,Qida, AU - Xu,Yanxia, AU - Wang,Huijuan, AU - Wang,Shigui, Y1 - 2016/10/11/ PY - 2016/07/12/received PY - 2016/10/06/revised PY - 2016/10/10/accepted PY - 2017/4/3/entrez PY - 2017/4/4/pubmed PY - 2017/6/22/medline KW - Chitin metabolism KW - Nilaparvata lugens KW - Trehalase KW - Validamycin KW - Wing development related genes KW - qRT-PCR SP - 81 EP - 90 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 137 N2 - Trehalase (TRE) is a key enzyme in trehalose degradation and has important functions in insect growth and chitin synthesis. Though validamycin has the potential for pest control by suppressing TRE activities, it is not known whether validamycin acts on both trehalose and chitin metabolism. TRE1 and TRE2 activities and glucose and glycogen contents decreased significantly after the injection of different doses of validamycin solution compared with the control group, while the trehalose content increased significantly. Overall, it showed that about 13 to 38% insects was appeared abnormal phenotypes, and 10 to 57% of insects died 48h after injection of solutions with different concentrations of validamycin; the chitin content also decreased significantly. Validamycin altered the relative expression levels of trehalose, glycogen and chitin metabolism-related genes by suppressing the activities of two TREs. We showed that the expression levels of three TRE and two trehalose-6-phosphate synthase (TPS) genes increased, while the expression levels of GP; CHS1 and its two transcripts, CHS1a, CHS1b; six chitinases, including Cht3, Cht4, Cht5, Cht6, Cht7, Cht9; and the HK, G6PI2, GFAT, GNPNA, PAGM1, UAP, VVL, CI and AP genes decreased significantly 48h after the injection of any validamycin concentration compared with the control group. These results demonstrate that by inhibiting the activities of two TREs, validamycin alters N. lugens chitin synthesis and degradation and affects trehalose and chitin metabolism-related gene expression. The development of TRE inhibitors may provide effective pest control in the future. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/28364808/Suppressing_the_activity_of_trehalase_with_validamycin_disrupts_the_trehalose_and_chitin_biosynthesis_pathways_in_the_rice_brown_planthopper_Nilaparvata_lugens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-3575(16)30122-5 DB - PRIME DP - Unbound Medicine ER -