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HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration.
Food Chem. 2017 Aug 15; 229:425-431.FC

Abstract

A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods. Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported. The limits of detection and quantification were calculated. Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values. PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey. Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey.

Authors+Show Affiliations

Chemical Laboratory of Lamia, Karaiskaki 85, Lamia 35100, Greece.Chemical Laboratory of Lamia, Karaiskaki 85, Lamia 35100, Greece.National and Kapodistrian University of Athens, Department of Chemistry, Food Chemistry Laboratory, Panepistimiopolis Zografou, 15771 Athens, Greece. Electronic address: iharpro@chem.uoa.gr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28372195

Citation

Pasias, Ioannis N., et al. "HMF and Diastase Activity in Honeys: a Fully Validated Approach and a Chemometric Analysis for Identification of Honey Freshness and Adulteration." Food Chemistry, vol. 229, 2017, pp. 425-431.
Pasias IN, Kiriakou IK, Proestos C. HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration. Food Chem. 2017;229:425-431.
Pasias, I. N., Kiriakou, I. K., & Proestos, C. (2017). HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration. Food Chemistry, 229, 425-431. https://doi.org/10.1016/j.foodchem.2017.02.084
Pasias IN, Kiriakou IK, Proestos C. HMF and Diastase Activity in Honeys: a Fully Validated Approach and a Chemometric Analysis for Identification of Honey Freshness and Adulteration. Food Chem. 2017 Aug 15;229:425-431. PubMed PMID: 28372195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - HMF and diastase activity in honeys: A fully validated approach and a chemometric analysis for identification of honey freshness and adulteration. AU - Pasias,Ioannis N, AU - Kiriakou,Ioannis K, AU - Proestos,Charalampos, Y1 - 2017/02/22/ PY - 2016/06/29/received PY - 2016/11/17/revised PY - 2017/02/17/accepted PY - 2017/4/5/entrez PY - 2017/4/5/pubmed PY - 2017/5/10/medline KW - Chemometrics KW - Cluster analysis KW - Diastase activity KW - HMF KW - Honey KW - PCA KW - Uncertainty SP - 425 EP - 431 JF - Food chemistry JO - Food Chem VL - 229 N2 - A fully validated approach for the determination of diastase activity and hydroxymethylfurfural content in honeys were presented in accordance with the official methods. Methods were performed in real honey sample analysis and due to the vast number of collected data sets reliable conclusions about the correlation between the composition and the quality criteria were exported. The limits of detection and quantification were calculated. Accuracy, precision and uncertainty were estimated for the first time in the kinetic and spectrometric techniques using the certified reference material and the determined values were in good accordance with the certified values. PCA and cluster analysis were performed in order to examine the correlation among the artificial feeding of honeybees with carbohydrate supplements and the chemical composition and properties of the honey. Diastase activity, sucrose content and hydroxymethylfurfural content were easily differentiated and these parameters were used for indication of the adulteration of the honey. SN - 1873-7072 UR - https://www.unboundmedicine.com/medline/citation/28372195/HMF_and_diastase_activity_in_honeys:_A_fully_validated_approach_and_a_chemometric_analysis_for_identification_of_honey_freshness_and_adulteration_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0308-8146(17)30287-X DB - PRIME DP - Unbound Medicine ER -