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Foliar-applied ethephon enhances the content of anthocyanin of black carrot roots (Daucus carota ssp. sativus var. atrorubens Alef.).
BMC Plant Biol. 2017 04 04; 17(1):70.BP

Abstract

BACKGROUND

Black carrots (Daucus carota ssp. sativus var. atrorubens Alef.) constitute a valuable source of anthocyanins, which are used as natural red, blue and purple food colourants. Anthocyanins and phenolic compounds are specialised metabolites, accumulation of which often requires elicitors, which act as molecular signals in plant stress responses. In the present study, ethephon, an ethylene-generating compound was explored as enhancer of anthocyanin and phenolic contents during growth of 'Deep Purple' black carrots. The effects of ethephon on several parameters were investigated, and the expression of biosynthetic anthocyanin genes was studied during growth and anthocyanin accumulation.

RESULTS

Roots of ethephon-treated carrot plants exhibited an increase in anthocyanin content of approximately 25%, with values ranging from 2.25 to 3.10 mg g[-1] fresh weight, compared with values ranging from 1.50 to 1.90 mg g[-1] fresh weight in untreated roots. The most rapid accumulation rate for anthocyanins, phenolic compounds, soluble solids and dry matter was observed between 10 and 13 weeks after sowing in both untreated and ethephon-treated carrots. The differences in anthocyanin contents between untreated and treated carrots increased for several weeks after the ethephon treatment was terminated. Five cyanidin-based anthocyanin forms were identified, with variable relative abundance values detected during root growth. Overall, the expression of the anthocyanin biosynthetic genes analysed (PAL1, PAL3, F3H1, DFR1, LDOX2) increased in response to ethephon treatment, as did the expression of the MYB1 transcription factor, which is associated with activation of the phenylpropanoid pathway under stress conditions. In addition, a correlation was proposed between ethylene and sugar contents and the induction of anthocyanin synthesis.

CONCLUSIONS

This study presents a novel method for enhancing anthocyanin content in black carrots. This finding is of economic importance as increased pigment concentration per unit of biomass implies improved profitability parameters in food colour production. We provide new insight into the accumulation patterns of the different cyanidin-based anthocyanins and phenolic compounds during root growth. Moreover, we show that enhanced anthocyanin content in ethephon-treated carrots is accompanied by increased expression of anthocyanin biosynthetic genes.

Authors+Show Affiliations

Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Hoejbakkegaard Alle 9-13, 2630, Taastrup, Denmark. grbe@plen.ku.dk.Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Hoejbakkegaard Alle 9-13, 2630, Taastrup, Denmark.DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871, Frederiksberg C, Denmark.Natural Colors Division, Chr. Hansen A/S, Agern Allé 24, 2970, Hørsholm, Denmark.Natural Colors Division, Chr. Hansen A/S, Agern Allé 24, 2970, Hørsholm, Denmark.Natural Colors Division, Chr. Hansen A/S, Agern Allé 24, 2970, Hørsholm, Denmark.Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Hoejbakkegaard Alle 9-13, 2630, Taastrup, Denmark.Section for Crop Sciences, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Hoejbakkegaard Alle 9-13, 2630, Taastrup, Denmark.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

28376712

Citation

Barba-Espín, Gregorio, et al. "Foliar-applied Ethephon Enhances the Content of Anthocyanin of Black Carrot Roots (Daucus Carota Ssp. Sativus Var. Atrorubens Alef.)." BMC Plant Biology, vol. 17, no. 1, 2017, p. 70.
Barba-Espín G, Glied S, Crocoll C, et al. Foliar-applied ethephon enhances the content of anthocyanin of black carrot roots (Daucus carota ssp. sativus var. atrorubens Alef.). BMC Plant Biol. 2017;17(1):70.
Barba-Espín, G., Glied, S., Crocoll, C., Dzhanfezova, T., Joernsgaard, B., Okkels, F., Lütken, H., & Müller, R. (2017). Foliar-applied ethephon enhances the content of anthocyanin of black carrot roots (Daucus carota ssp. sativus var. atrorubens Alef.). BMC Plant Biology, 17(1), 70. https://doi.org/10.1186/s12870-017-1021-7
Barba-Espín G, et al. Foliar-applied Ethephon Enhances the Content of Anthocyanin of Black Carrot Roots (Daucus Carota Ssp. Sativus Var. Atrorubens Alef.). BMC Plant Biol. 2017 04 4;17(1):70. PubMed PMID: 28376712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Foliar-applied ethephon enhances the content of anthocyanin of black carrot roots (Daucus carota ssp. sativus var. atrorubens Alef.). AU - Barba-Espín,Gregorio, AU - Glied,Stephan, AU - Crocoll,Christoph, AU - Dzhanfezova,Tsaneta, AU - Joernsgaard,Bjarne, AU - Okkels,Finn, AU - Lütken,Henrik, AU - Müller,Renate, Y1 - 2017/04/04/ PY - 2016/12/19/received PY - 2017/03/29/accepted PY - 2017/4/6/entrez PY - 2017/4/6/pubmed PY - 2017/9/28/medline KW - Anthocyanin accumulation KW - Black carrot KW - Ethephon KW - Ethylene KW - Gene expression SP - 70 EP - 70 JF - BMC plant biology JO - BMC Plant Biol VL - 17 IS - 1 N2 - BACKGROUND: Black carrots (Daucus carota ssp. sativus var. atrorubens Alef.) constitute a valuable source of anthocyanins, which are used as natural red, blue and purple food colourants. Anthocyanins and phenolic compounds are specialised metabolites, accumulation of which often requires elicitors, which act as molecular signals in plant stress responses. In the present study, ethephon, an ethylene-generating compound was explored as enhancer of anthocyanin and phenolic contents during growth of 'Deep Purple' black carrots. The effects of ethephon on several parameters were investigated, and the expression of biosynthetic anthocyanin genes was studied during growth and anthocyanin accumulation. RESULTS: Roots of ethephon-treated carrot plants exhibited an increase in anthocyanin content of approximately 25%, with values ranging from 2.25 to 3.10 mg g[-1] fresh weight, compared with values ranging from 1.50 to 1.90 mg g[-1] fresh weight in untreated roots. The most rapid accumulation rate for anthocyanins, phenolic compounds, soluble solids and dry matter was observed between 10 and 13 weeks after sowing in both untreated and ethephon-treated carrots. The differences in anthocyanin contents between untreated and treated carrots increased for several weeks after the ethephon treatment was terminated. Five cyanidin-based anthocyanin forms were identified, with variable relative abundance values detected during root growth. Overall, the expression of the anthocyanin biosynthetic genes analysed (PAL1, PAL3, F3H1, DFR1, LDOX2) increased in response to ethephon treatment, as did the expression of the MYB1 transcription factor, which is associated with activation of the phenylpropanoid pathway under stress conditions. In addition, a correlation was proposed between ethylene and sugar contents and the induction of anthocyanin synthesis. CONCLUSIONS: This study presents a novel method for enhancing anthocyanin content in black carrots. This finding is of economic importance as increased pigment concentration per unit of biomass implies improved profitability parameters in food colour production. We provide new insight into the accumulation patterns of the different cyanidin-based anthocyanins and phenolic compounds during root growth. Moreover, we show that enhanced anthocyanin content in ethephon-treated carrots is accompanied by increased expression of anthocyanin biosynthetic genes. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/28376712/Foliar_applied_ethephon_enhances_the_content_of_anthocyanin_of_black_carrot_roots__Daucus_carota_ssp__sativus_var__atrorubens_Alef___ DB - PRIME DP - Unbound Medicine ER -