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Induction of angiotensin converting enzyme in cultured human monocytes by a factor present in the bronchoalveolar lavage fluid of sarcoidosis patients.
Sarcoidosis. 1988 Mar; 5(1):17-23.S

Abstract

Human peripheral blood monocytes synthesize a low level of angiotensin converting enzyme (ACE) when cultured in vitro for six days. If cell-free bronchoalveolar lavage fluid from sarcoidosis patients was included with the monocytes during culture, the monocyte ACE level increased significantly in a dose dependent manner. In contrast, the cell-free bronchoalveolar lavage fluid from control subjects and patients with idiopathic pulmonary fibrosis or hypersensitivity pneumonitis raised basal monocyte levels little, if at all. These results suggested that sarcoid lavage fluid contains a soluble ACE-inducing factor (AIF) that was absent or present in much lower concentrations in the bronchoalveolar lavage fluid of the other patients studied. Initial attempts to characterize this AIF indicated an apparent molecular weight of greater than 5000 daltons as determined by Amicon ultrafiltration. The AIF was stable to heating at 56 degrees for 1 hour. This finding demonstrated that lavage ACE was not responsible for the AIF activity since lavage ACE is inactivated under these conditions. Gamma-interferon (macrophage-activating factor) was also eliminated as the agent responsible for AIF activity since gamma-interferon decreased rather than increased monocyte ACE. In summary, bronchoalveolar lavage fluid from sarcoid patients contains a soluble factor (AIF) that induces ACE in cultured monocytes. Action of this factor in vivo may be responsible for the increased ACE levels seen in the monocyte-derived epithelioid granuloma cells of sarcoidosis.

Authors+Show Affiliations

Thoracic Diseases Research Unit, Mayo Clinic, Rochester, MN 55905.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2837820

Citation

Rohrbach, M S., et al. "Induction of Angiotensin Converting Enzyme in Cultured Human Monocytes By a Factor Present in the Bronchoalveolar Lavage Fluid of Sarcoidosis Patients." Sarcoidosis, vol. 5, no. 1, 1988, pp. 17-23.
Rohrbach MS, Vuk-Pavlović Z, Martin WJ, et al. Induction of angiotensin converting enzyme in cultured human monocytes by a factor present in the bronchoalveolar lavage fluid of sarcoidosis patients. Sarcoidosis. 1988;5(1):17-23.
Rohrbach, M. S., Vuk-Pavlović, Z., Martin, W. J., DeLaval, P., & Genetet, N. (1988). Induction of angiotensin converting enzyme in cultured human monocytes by a factor present in the bronchoalveolar lavage fluid of sarcoidosis patients. Sarcoidosis, 5(1), 17-23.
Rohrbach MS, et al. Induction of Angiotensin Converting Enzyme in Cultured Human Monocytes By a Factor Present in the Bronchoalveolar Lavage Fluid of Sarcoidosis Patients. Sarcoidosis. 1988;5(1):17-23. PubMed PMID: 2837820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Induction of angiotensin converting enzyme in cultured human monocytes by a factor present in the bronchoalveolar lavage fluid of sarcoidosis patients. AU - Rohrbach,M S, AU - Vuk-Pavlović,Z, AU - Martin,W J, AU - DeLaval,P, AU - Genetet,N, PY - 1988/3/1/pubmed PY - 1988/3/1/medline PY - 1988/3/1/entrez SP - 17 EP - 23 JF - Sarcoidosis JO - Sarcoidosis VL - 5 IS - 1 N2 - Human peripheral blood monocytes synthesize a low level of angiotensin converting enzyme (ACE) when cultured in vitro for six days. If cell-free bronchoalveolar lavage fluid from sarcoidosis patients was included with the monocytes during culture, the monocyte ACE level increased significantly in a dose dependent manner. In contrast, the cell-free bronchoalveolar lavage fluid from control subjects and patients with idiopathic pulmonary fibrosis or hypersensitivity pneumonitis raised basal monocyte levels little, if at all. These results suggested that sarcoid lavage fluid contains a soluble ACE-inducing factor (AIF) that was absent or present in much lower concentrations in the bronchoalveolar lavage fluid of the other patients studied. Initial attempts to characterize this AIF indicated an apparent molecular weight of greater than 5000 daltons as determined by Amicon ultrafiltration. The AIF was stable to heating at 56 degrees for 1 hour. This finding demonstrated that lavage ACE was not responsible for the AIF activity since lavage ACE is inactivated under these conditions. Gamma-interferon (macrophage-activating factor) was also eliminated as the agent responsible for AIF activity since gamma-interferon decreased rather than increased monocyte ACE. In summary, bronchoalveolar lavage fluid from sarcoid patients contains a soluble factor (AIF) that induces ACE in cultured monocytes. Action of this factor in vivo may be responsible for the increased ACE levels seen in the monocyte-derived epithelioid granuloma cells of sarcoidosis. SN - 0393-1447 UR - https://www.unboundmedicine.com/medline/citation/2837820/Induction_of_angiotensin_converting_enzyme_in_cultured_human_monocytes_by_a_factor_present_in_the_bronchoalveolar_lavage_fluid_of_sarcoidosis_patients_ L2 - http://www.diseaseinfosearch.org/result/6412 DB - PRIME DP - Unbound Medicine ER -