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Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015).
Arch Virol 2017; 162(8):2363-2367AV

Abstract

Orf or contagious ecthyma is a neglected and economically important zoonotic disease caused by a dermatotropic parapoxvirus that commonly affects domestic small ruminants. Although orf is globally distributed, there is a paucity of information on the disease in many African countries. Here, a suspected severe outbreak of orf in goats at a farm in Lusaka was investigated. Orf virus (ORFV) infection was confirmed by PCR amplification of viral DNA (RNA polymerase, B2L and virus interferon-resistance genes) in clinical samples. Some detected genes were sequenced and phylogenetically analyzed. This is the first report on molecular characterization of ORFV in goats in Zambia.

Authors+Show Affiliations

Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia. esikabala@yahoo.com.Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia.Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia.Department of Biomedical Sciences, School of Health Sciences, University of Zambia, PO Box 50110, Lusaka, Zambia.Department of Clinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia. Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan. Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan.Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28378295

Citation

Simulundu, Edgar, et al. "Genetic Characterization of Orf Virus Associated With an Outbreak of Severe Orf in Goats at a Farm in Lusaka, Zambia (2015)." Archives of Virology, vol. 162, no. 8, 2017, pp. 2363-2367.
Simulundu E, Mtine N, Kapalamula TF, et al. Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015). Arch Virol. 2017;162(8):2363-2367.
Simulundu, E., Mtine, N., Kapalamula, T. F., Kajihara, M., Qiu, Y., Ngoma, J., ... Mweene, A. S. (2017). Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015). Archives of Virology, 162(8), pp. 2363-2367. doi:10.1007/s00705-017-3352-y.
Simulundu E, et al. Genetic Characterization of Orf Virus Associated With an Outbreak of Severe Orf in Goats at a Farm in Lusaka, Zambia (2015). Arch Virol. 2017;162(8):2363-2367. PubMed PMID: 28378295.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015). AU - Simulundu,Edgar, AU - Mtine,Nandi, AU - Kapalamula,Thoko F, AU - Kajihara,Masahiro, AU - Qiu,Yongjin, AU - Ngoma,James, AU - Zulu,Victor, AU - Kwenda,Geoffrey, AU - Chisanga,Chrispin, AU - Phiri,Isaac K, AU - Takada,Ayato, AU - Mweene,Aaron S, Y1 - 2017/04/04/ PY - 2017/01/10/received PY - 2017/02/24/accepted PY - 2017/4/6/pubmed PY - 2017/8/2/medline PY - 2017/4/6/entrez SP - 2363 EP - 2367 JF - Archives of virology JO - Arch. Virol. VL - 162 IS - 8 N2 - Orf or contagious ecthyma is a neglected and economically important zoonotic disease caused by a dermatotropic parapoxvirus that commonly affects domestic small ruminants. Although orf is globally distributed, there is a paucity of information on the disease in many African countries. Here, a suspected severe outbreak of orf in goats at a farm in Lusaka was investigated. Orf virus (ORFV) infection was confirmed by PCR amplification of viral DNA (RNA polymerase, B2L and virus interferon-resistance genes) in clinical samples. Some detected genes were sequenced and phylogenetically analyzed. This is the first report on molecular characterization of ORFV in goats in Zambia. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/28378295/Genetic_characterization_of_orf_virus_associated_with_an_outbreak_of_severe_orf_in_goats_at_a_farm_in_Lusaka_Zambia__2015__ L2 - https://dx.doi.org/10.1007/s00705-017-3352-y DB - PRIME DP - Unbound Medicine ER -