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Immunohistochemical evaluation of the influence of L-arginine on biomarkers of environmental (cellular) stress in the kidneys of pregnant female rats.
Ann Agric Environ Med 2017; 24(1):121-128AA

Abstract

INTRODUCTION

Kidney damage during pregnancy constitutes a diagnostic and therapeutic challenge. However, it is not entirely known whether a kidney condition recognised before the pregnancy releases an organism's response to pregnancy, or whether pregnancy itself worsens kidney function.

OBJECTIVE

The aim of the study was immunohistochemical evaluation of cells of kidneys of pregnant rats under the influence of nitric oxide (NO), with measurement of the immunoexpression of cellular stress markers (p-53, HSP 70). The dose of administered L-arginine (NO substrate) was approximated to that applied in obstetrics in gestosis prevention and treatment in pregnant women.

MATERIALS AND METHOD

60 female rats used in experiment were divided into 6 groups: 3 experimental and 3 control. The females from experimental groups were administered L-arginine (40g/kg, per os) every other day starting from the seventh day or pregnancy. The animals were decapitated on the 10th, 20th day of pregnancy, and 10 days after the delivery. Kidneys taken from decapitated rats were evaluated using the immunohistochemical three step method. HSP 70 and p-53 proteins were detected.

CONCLUSIONS

L-arginine increased the expression of p-53 protein - on the 10th day of pregnancy, which increased at the end of pregnancy; however, 10 days after delivery the level dropped below that observable during physiological pregnancy. Hormonal changes in physiological pregnancy cause an increase in expression of the p-53 (cell stress marker) in the epithelial cells of renal tubules, mainly at the end of pregnancy (20th day). 10 days after the delivery, this expression decreases. The expression of HSP-70 protein increases already on the 10th day of pregnancy and maintains a similar level throughout the pregnancy, but is reduced after the puerperium.

Authors+Show Affiliations

Department of Histology and Embryology medical University in Lublin, Poland.Department of Maritime and Hyperbaric Medicine Military Institute of Medicine, Gdynia, Poland.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28378970

Citation

Pedrycz, Agnieszka, and Piotr Siermontowski. "Immunohistochemical Evaluation of the Influence of L-arginine On Biomarkers of Environmental (cellular) Stress in the Kidneys of Pregnant Female Rats." Annals of Agricultural and Environmental Medicine : AAEM, vol. 24, no. 1, 2017, pp. 121-128.
Pedrycz A, Siermontowski P. Immunohistochemical evaluation of the influence of L-arginine on biomarkers of environmental (cellular) stress in the kidneys of pregnant female rats. Ann Agric Environ Med. 2017;24(1):121-128.
Pedrycz, A., & Siermontowski, P. (2017). Immunohistochemical evaluation of the influence of L-arginine on biomarkers of environmental (cellular) stress in the kidneys of pregnant female rats. Annals of Agricultural and Environmental Medicine : AAEM, 24(1), pp. 121-128. doi:10.5604/12321966.1230677.
Pedrycz A, Siermontowski P. Immunohistochemical Evaluation of the Influence of L-arginine On Biomarkers of Environmental (cellular) Stress in the Kidneys of Pregnant Female Rats. Ann Agric Environ Med. 2017 Mar 31;24(1):121-128. PubMed PMID: 28378970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunohistochemical evaluation of the influence of L-arginine on biomarkers of environmental (cellular) stress in the kidneys of pregnant female rats. AU - Pedrycz,Agnieszka, AU - Siermontowski,Piotr, PY - 2017/4/6/entrez PY - 2017/4/6/pubmed PY - 2017/4/20/medline SP - 121 EP - 128 JF - Annals of agricultural and environmental medicine : AAEM JO - Ann Agric Environ Med VL - 24 IS - 1 N2 - INTRODUCTION: Kidney damage during pregnancy constitutes a diagnostic and therapeutic challenge. However, it is not entirely known whether a kidney condition recognised before the pregnancy releases an organism's response to pregnancy, or whether pregnancy itself worsens kidney function. OBJECTIVE: The aim of the study was immunohistochemical evaluation of cells of kidneys of pregnant rats under the influence of nitric oxide (NO), with measurement of the immunoexpression of cellular stress markers (p-53, HSP 70). The dose of administered L-arginine (NO substrate) was approximated to that applied in obstetrics in gestosis prevention and treatment in pregnant women. MATERIALS AND METHOD: 60 female rats used in experiment were divided into 6 groups: 3 experimental and 3 control. The females from experimental groups were administered L-arginine (40g/kg, per os) every other day starting from the seventh day or pregnancy. The animals were decapitated on the 10th, 20th day of pregnancy, and 10 days after the delivery. Kidneys taken from decapitated rats were evaluated using the immunohistochemical three step method. HSP 70 and p-53 proteins were detected. CONCLUSIONS: L-arginine increased the expression of p-53 protein - on the 10th day of pregnancy, which increased at the end of pregnancy; however, 10 days after delivery the level dropped below that observable during physiological pregnancy. Hormonal changes in physiological pregnancy cause an increase in expression of the p-53 (cell stress marker) in the epithelial cells of renal tubules, mainly at the end of pregnancy (20th day). 10 days after the delivery, this expression decreases. The expression of HSP-70 protein increases already on the 10th day of pregnancy and maintains a similar level throughout the pregnancy, but is reduced after the puerperium. SN - 1898-2263 UR - https://www.unboundmedicine.com/medline/citation/28378970/Immunohistochemical_evaluation_of_the_influence_of_L-arginine_on_biomarkers_of_environmental_(cellular)_stress_in_the_kidneys_of_pregnant_female_rats L2 - https://doi.org/10.5604/12321966.1230677 DB - PRIME DP - Unbound Medicine ER -