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Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia coli Isolates from Chickens in China.
Foodborne Pathog Dis. 2017 04; 14(4):210-218.FP

Abstract

The aim of this study was to investigate the prevalence of fosfomycin resistance gene fosA3 and characterize plasmids harboring fosA3 among CTX-M-producing Escherichia coli from chickens in China. A total of 234 CTX-M-producing E. coli isolates collected from chickens from 2014 to 2016 were screened for the presence of plasmid-mediated fosfomycin resistance genes (fosA, fosA3, and fosC2). Clonal relatedness of fosA3-positive isolates was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The genetic environment of fosA3 was analyzed by polymerase chain reaction (PCR) mapping. Plasmids were studied by using conjugation experiments, PCR-based replicon typing and plasmid MLST. Sixty-four (27.4%) fosA3-positive E. coli isolates were identified in this study. The gene blaCTX-M-55 (31/64) was predominant among these strains, followed by blaCTX-M-14 (18/64) and blaCTX-M-65 (14/64). Various PFGE patterns and sequence types (STs) indicated that these isolates were clonally unrelated. Seven different genetic environments of fosA3 were identified and two new combinations (ISEcp1-blaCTX-M-65-ΔIS903D-IS26-fosA3-orf1-orf2-Δorf3-IS26 and IS26-ISEcp1-blaCTX-M-3-orf477-blaTEM-1-IS26-fosA3-orf1-orf2-Δorf3-IS26) were discovered for the first time. Conjugation experiments were successful for 47 isolates and 33 transconjugants harbored a single plasmid. Plasmids carrying fosA3 belonged to incompatibility group IncFII (17/33), IncI1 (2/33), IncHI2 (3/33), and IncB/O (1/33). F33:A-:B- plasmids carrying blaCTX-M-55, IncHI2/ST3 plasmids carrying blaCTX-M-65, and F2:A-:B-plasmids carrying blaCTX-M-55 were found in E. coli isolates from different provinces. Our results revealed a considerable prevalence of fosA3 gene among CTX-M-producing E. coli with clonal diversity from chickens in China. The transmission of different kinds of plasmids is responsible for the dissemination of fosA3 in chicken farms in China.

Authors+Show Affiliations

1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China .1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University , Chengdu, China . 2 Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province , Chengdu, China . 3 "985 Project" Science Innovative Platform for Resource and Environment Protection of Southwestern China, Sichuan University , Chengdu, China .

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28379732

Citation

Jiang, Wei, et al. "Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia Coli Isolates From Chickens in China." Foodborne Pathogens and Disease, vol. 14, no. 4, 2017, pp. 210-218.
Jiang W, Men S, Kong L, et al. Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia coli Isolates from Chickens in China. Foodborne Pathog Dis. 2017;14(4):210-218.
Jiang, W., Men, S., Kong, L., Ma, S., Yang, Y., Wang, Y., Yuan, Q., Cheng, G., Zou, W., & Wang, H. (2017). Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia coli Isolates from Chickens in China. Foodborne Pathogens and Disease, 14(4), 210-218. https://doi.org/10.1089/fpd.2016.2230
Jiang W, et al. Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia Coli Isolates From Chickens in China. Foodborne Pathog Dis. 2017;14(4):210-218. PubMed PMID: 28379732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prevalence of Plasmid-Mediated Fosfomycin Resistance Gene fosA3 Among CTX-M-Producing Escherichia coli Isolates from Chickens in China. AU - Jiang,Wei, AU - Men,Shuai, AU - Kong,Linghan, AU - Ma,Suzhen, AU - Yang,Yongqiang, AU - Wang,Yongxiang, AU - Yuan,Qiwu, AU - Cheng,Guangyang, AU - Zou,Wencheng, AU - Wang,Hongning, Y1 - 2017/02/17/ PY - 2017/4/6/entrez PY - 2017/4/6/pubmed PY - 2017/10/17/medline KW - Gram-negative bacteria KW - extended spectrum beta-lactamases KW - genetic environments KW - plasmid analysis SP - 210 EP - 218 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 14 IS - 4 N2 - The aim of this study was to investigate the prevalence of fosfomycin resistance gene fosA3 and characterize plasmids harboring fosA3 among CTX-M-producing Escherichia coli from chickens in China. A total of 234 CTX-M-producing E. coli isolates collected from chickens from 2014 to 2016 were screened for the presence of plasmid-mediated fosfomycin resistance genes (fosA, fosA3, and fosC2). Clonal relatedness of fosA3-positive isolates was determined by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The genetic environment of fosA3 was analyzed by polymerase chain reaction (PCR) mapping. Plasmids were studied by using conjugation experiments, PCR-based replicon typing and plasmid MLST. Sixty-four (27.4%) fosA3-positive E. coli isolates were identified in this study. The gene blaCTX-M-55 (31/64) was predominant among these strains, followed by blaCTX-M-14 (18/64) and blaCTX-M-65 (14/64). Various PFGE patterns and sequence types (STs) indicated that these isolates were clonally unrelated. Seven different genetic environments of fosA3 were identified and two new combinations (ISEcp1-blaCTX-M-65-ΔIS903D-IS26-fosA3-orf1-orf2-Δorf3-IS26 and IS26-ISEcp1-blaCTX-M-3-orf477-blaTEM-1-IS26-fosA3-orf1-orf2-Δorf3-IS26) were discovered for the first time. Conjugation experiments were successful for 47 isolates and 33 transconjugants harbored a single plasmid. Plasmids carrying fosA3 belonged to incompatibility group IncFII (17/33), IncI1 (2/33), IncHI2 (3/33), and IncB/O (1/33). F33:A-:B- plasmids carrying blaCTX-M-55, IncHI2/ST3 plasmids carrying blaCTX-M-65, and F2:A-:B-plasmids carrying blaCTX-M-55 were found in E. coli isolates from different provinces. Our results revealed a considerable prevalence of fosA3 gene among CTX-M-producing E. coli with clonal diversity from chickens in China. The transmission of different kinds of plasmids is responsible for the dissemination of fosA3 in chicken farms in China. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/28379732/Prevalence_of_Plasmid_Mediated_Fosfomycin_Resistance_Gene_fosA3_Among_CTX_M_Producing_Escherichia_coli_Isolates_from_Chickens_in_China_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2016.2230?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -