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Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species.
PLoS One. 2017; 12(4):e0175081.Plos

Abstract

Coccidioides is a soil-dwelling fungus that causes coccidioidomycosis, a disease also known as Valley fever, which affects humans and a variety of animal species. Recent findings of Coccidioides in new, unexpected areas of the United States have demonstrated the need for a better understanding of its geographic distribution. Large serological studies on animals could provide important information on the geographic distribution of this pathogen. To facilitate such studies, we used protein A/G, a recombinant protein that binds IgG antibodies from a variety of mammalian species, to develop an enzyme immunoassay (EIA) that detects IgG antibodies against Coccidioides in a highly sensitive and high-throughput manner. We showed the potential of this assay to be adapted to multiple animal species by testing a collection of serum and/or plasma samples from dogs, mice, and humans with or without confirmed coccidioidomycosis. We then evaluated the performance of the assay in dogs, using sera from dogs residing in a highly endemic area, and found seropositivity rates significantly higher than those in dogs of non-endemic areas. We further evaluated the specificity of the assay in dogs infected with other fungal pathogens known to cross-react with Coccidioides. Finally, we used the assay to perform a cross-sectional serosurvey investigating dogs from Washington, a state in which infection with Coccidioides has recently been documented. In summary, we have developed a Coccidioides EIA for the detection of antibodies in canines that is more sensitive and has higher throughput than currently available methods, and by testing this assay in mice and humans, we have shown a proof of principle of its adaptability for other animal species.

Authors+Show Affiliations

Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.Zoonotic Disease Program, Office of Environmental Public Health Sciences, Washington State Department of Health, Olympia, Washington, United States of America.Zoonotic Disease Program, Office of Environmental Public Health Sciences, Washington State Department of Health, Olympia, Washington, United States of America.Zoonotic Disease Program, Office of Environmental Public Health Sciences, Washington State Department of Health, Olympia, Washington, United States of America.Arizona Department of Health Services, Phoenix, Arizona, United States of America.Arizona Department of Health Services, Phoenix, Arizona, United States of America.Arizona Department of Health Services, Phoenix, Arizona, United States of America.MiraVista Diagnostics, Indianapolis, Indiana, United States of America.Oregon Veterinary Diagnostic Laboratory, Oregon State University, Corvallis, Oregon, United States of America.Valley Fever Center for Excellence, University of Arizona, Tucson, Arizona, United States of America.Biostatistics and Information Management Office, Division of Foodborne, Waterborne and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

28380017

Citation

Chow, Nancy A., et al. "Development of an Enzyme Immunoassay for Detection of Antibodies Against Coccidioides in Dogs and Other Mammalian Species." PloS One, vol. 12, no. 4, 2017, pp. e0175081.
Chow NA, Lindsley MD, McCotter OZ, et al. Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species. PLoS One. 2017;12(4):e0175081.
Chow, N. A., Lindsley, M. D., McCotter, O. Z., Kangiser, D., Wohrle, R. D., Clifford, W. R., Yaglom, H. D., Adams, L. E., Komatsu, K., Durkin, M. M., Baker, R. J., Shubitz, L. F., Derado, G., Chiller, T. M., & Litvintseva, A. P. (2017). Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species. PloS One, 12(4), e0175081. https://doi.org/10.1371/journal.pone.0175081
Chow NA, et al. Development of an Enzyme Immunoassay for Detection of Antibodies Against Coccidioides in Dogs and Other Mammalian Species. PLoS One. 2017;12(4):e0175081. PubMed PMID: 28380017.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an enzyme immunoassay for detection of antibodies against Coccidioides in dogs and other mammalian species. AU - Chow,Nancy A, AU - Lindsley,Mark D, AU - McCotter,Orion Z, AU - Kangiser,Dave, AU - Wohrle,Ron D, AU - Clifford,Wayne R, AU - Yaglom,Hayley D, AU - Adams,Laura E, AU - Komatsu,Kenneth, AU - Durkin,Michelle M, AU - Baker,Rocky J, AU - Shubitz,Lisa F, AU - Derado,Gordana, AU - Chiller,Tom M, AU - Litvintseva,Anastasia P, Y1 - 2017/04/05/ PY - 2017/01/10/received PY - 2017/03/20/accepted PY - 2017/4/6/entrez PY - 2017/4/6/pubmed PY - 2017/9/7/medline SP - e0175081 EP - e0175081 JF - PloS one JO - PLoS One VL - 12 IS - 4 N2 - Coccidioides is a soil-dwelling fungus that causes coccidioidomycosis, a disease also known as Valley fever, which affects humans and a variety of animal species. Recent findings of Coccidioides in new, unexpected areas of the United States have demonstrated the need for a better understanding of its geographic distribution. Large serological studies on animals could provide important information on the geographic distribution of this pathogen. To facilitate such studies, we used protein A/G, a recombinant protein that binds IgG antibodies from a variety of mammalian species, to develop an enzyme immunoassay (EIA) that detects IgG antibodies against Coccidioides in a highly sensitive and high-throughput manner. We showed the potential of this assay to be adapted to multiple animal species by testing a collection of serum and/or plasma samples from dogs, mice, and humans with or without confirmed coccidioidomycosis. We then evaluated the performance of the assay in dogs, using sera from dogs residing in a highly endemic area, and found seropositivity rates significantly higher than those in dogs of non-endemic areas. We further evaluated the specificity of the assay in dogs infected with other fungal pathogens known to cross-react with Coccidioides. Finally, we used the assay to perform a cross-sectional serosurvey investigating dogs from Washington, a state in which infection with Coccidioides has recently been documented. In summary, we have developed a Coccidioides EIA for the detection of antibodies in canines that is more sensitive and has higher throughput than currently available methods, and by testing this assay in mice and humans, we have shown a proof of principle of its adaptability for other animal species. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/28380017/Development_of_an_enzyme_immunoassay_for_detection_of_antibodies_against_Coccidioides_in_dogs_and_other_mammalian_species_ L2 - https://dx.plos.org/10.1371/journal.pone.0175081 DB - PRIME DP - Unbound Medicine ER -