Tags

Type your tag names separated by a space and hit enter

An intermediate in transposition of the conjugative transposon Tn916.
Proc Natl Acad Sci U S A. 1988 Jul; 85(13):4809-13.PN

Abstract

Using the conjugative transposon Tn916, we have identified a closed covalent circular form produced in vivo that is able to serve as an intermediate in transposition. When a region of a streptococcal chromosome containing Tn916 is cloned in an Escherichia coli plasmid, supercoiled transposon molecules are excised spontaneously. The purified supercoiled forms transform Bacillus subtilis protoplasts by inserting into the chromosome, apparently at random. In B. subtilis, Tn916 retains its ability to promote conjugative transposition, as shown by its transfer to Streptococcus pyogenes.

Authors+Show Affiliations

Department of Microbiology and Immunology, Emory University Health Sciences Center, Atlanta, GA 30322.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2838847

Citation

Scott, J R., et al. "An Intermediate in Transposition of the Conjugative Transposon Tn916." Proceedings of the National Academy of Sciences of the United States of America, vol. 85, no. 13, 1988, pp. 4809-13.
Scott JR, Kirchman PA, Caparon MG. An intermediate in transposition of the conjugative transposon Tn916. Proc Natl Acad Sci USA. 1988;85(13):4809-13.
Scott, J. R., Kirchman, P. A., & Caparon, M. G. (1988). An intermediate in transposition of the conjugative transposon Tn916. Proceedings of the National Academy of Sciences of the United States of America, 85(13), 4809-13.
Scott JR, Kirchman PA, Caparon MG. An Intermediate in Transposition of the Conjugative Transposon Tn916. Proc Natl Acad Sci USA. 1988;85(13):4809-13. PubMed PMID: 2838847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An intermediate in transposition of the conjugative transposon Tn916. AU - Scott,J R, AU - Kirchman,P A, AU - Caparon,M G, PY - 1988/7/1/pubmed PY - 1988/7/1/medline PY - 1988/7/1/entrez SP - 4809 EP - 13 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc. Natl. Acad. Sci. U.S.A. VL - 85 IS - 13 N2 - Using the conjugative transposon Tn916, we have identified a closed covalent circular form produced in vivo that is able to serve as an intermediate in transposition. When a region of a streptococcal chromosome containing Tn916 is cloned in an Escherichia coli plasmid, supercoiled transposon molecules are excised spontaneously. The purified supercoiled forms transform Bacillus subtilis protoplasts by inserting into the chromosome, apparently at random. In B. subtilis, Tn916 retains its ability to promote conjugative transposition, as shown by its transfer to Streptococcus pyogenes. SN - 0027-8424 UR - https://www.unboundmedicine.com/medline/citation/2838847/An_intermediate_in_transposition_of_the_conjugative_transposon_Tn916_ L2 - http://www.pnas.org/cgi/pmidlookup?view=long&pmid=2838847 DB - PRIME DP - Unbound Medicine ER -