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Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica.
Methods Mol Biol. 2017; 1593:47-71.MM

Abstract

There are more than 70 genes in the flagellar and chemosensory regulon of Salmonella enterica. These genes are organized into a transcriptional hierarchy of three promoter classes. At the top of the transcriptional hierarchy is the flhDC operon, also called the flagellar master operon, which is transcribed from the flagellar class 1 promoter region. The protein products of the flhDC operon form a hetero-multimeric complex, FlhD4C2, which directs σ70 RNA polymerase to transcribe from class 2 flagellar promoters. Products of flagellar class 2 transcription are required for the structure and assembly of the hook-basal body (HBB) complex. One of the class 2 flagellar genes, fliA, encodes an alternative sigma transcription factor, σ28, which directs transcription from flagellar class 3 promoters. The class 3 promoters direct transcription of gene products needed after HBB completion including the motor force generators, the filament, and the chemosensory genes. Flagellar gene transcription is coupled to assembly at the level of hook-basal body completion. Two key proteins, σ28 and FliT, play assembly roles prior to HBB completion and upon HBB completion act as positive and negative regulators, respectively. HBB completion signals a secretion-specificity switch in the flagellar type III secretion system, which results in the secretion of σ28 and FliT antigonists allowing these proteins to perform their roles in transcriptional regulation of flagellar genes. Genetic methods have provided the principle driving forces in our understanding of how flagellar gene expression is controlled and coupled to the assembly process.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Chevance FF
Department of Biology, University of Utah, 257 1400 E. #201, Salt Lake City, UT, 84112, USA.
Hughes KT
Department of Biology, University of Utah, 257 1400 E. #201, Salt Lake City, UT, 84112, USA. Kelly.hughes@utah.edu.

MeSH

Bacterial ProteinsDNA-Directed RNA PolymerasesFlagellaGene Expression Regulation, BacterialGenes, BacterialOperonPromoter Regions, GeneticSalmonella entericaSigma FactorTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

28389944

Citation

Chevance, Fabienne F V., and Kelly T. Hughes. "Coupling of Flagellar Gene Expression With Assembly in Salmonella Enterica." Methods in Molecular Biology (Clifton, N.J.), vol. 1593, 2017, pp. 47-71.
Chevance FF, Hughes KT. Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica. Methods Mol Biol. 2017;1593:47-71.
Chevance, F. F., & Hughes, K. T. (2017). Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica. Methods in Molecular Biology (Clifton, N.J.), 1593, 47-71. https://doi.org/10.1007/978-1-4939-6927-2_4
Chevance FF, Hughes KT. Coupling of Flagellar Gene Expression With Assembly in Salmonella Enterica. Methods Mol Biol. 2017;1593:47-71. PubMed PMID: 28389944.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coupling of Flagellar Gene Expression with Assembly in Salmonella enterica. AU - Chevance,Fabienne F V, AU - Hughes,Kelly T, PY - 2017/4/9/entrez PY - 2017/4/9/pubmed PY - 2018/2/7/medline KW - Flagellar gene expression KW - Gene regulation KW - Genetic manipulations KW - Real-time PCR KW - Transposable elements KW - β-Galactosidase assays KW - λ-Red SP - 47 EP - 71 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1593 N2 - There are more than 70 genes in the flagellar and chemosensory regulon of Salmonella enterica. These genes are organized into a transcriptional hierarchy of three promoter classes. At the top of the transcriptional hierarchy is the flhDC operon, also called the flagellar master operon, which is transcribed from the flagellar class 1 promoter region. The protein products of the flhDC operon form a hetero-multimeric complex, FlhD4C2, which directs σ70 RNA polymerase to transcribe from class 2 flagellar promoters. Products of flagellar class 2 transcription are required for the structure and assembly of the hook-basal body (HBB) complex. One of the class 2 flagellar genes, fliA, encodes an alternative sigma transcription factor, σ28, which directs transcription from flagellar class 3 promoters. The class 3 promoters direct transcription of gene products needed after HBB completion including the motor force generators, the filament, and the chemosensory genes. Flagellar gene transcription is coupled to assembly at the level of hook-basal body completion. Two key proteins, σ28 and FliT, play assembly roles prior to HBB completion and upon HBB completion act as positive and negative regulators, respectively. HBB completion signals a secretion-specificity switch in the flagellar type III secretion system, which results in the secretion of σ28 and FliT antigonists allowing these proteins to perform their roles in transcriptional regulation of flagellar genes. Genetic methods have provided the principle driving forces in our understanding of how flagellar gene expression is controlled and coupled to the assembly process. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/28389944/Coupling_of_Flagellar_Gene_Expression_with_Assembly_in_Salmonella_enterica_ L2 - https://dx.doi.org/10.1007/978-1-4939-6927-2_4 DB - PRIME DP - Unbound Medicine ER -